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The annotation and conditions in this rule are derived from the following entries: P50465 (END8_ECOLI)

If a protein meets these conditions... i

Common conditions

Special conditions

    • Subsequence at position 1 - 1 aligns to entry P50465 (individually applies "Removed")
    • Subsequence at position 2 - 2 aligns to "P" in entry P50465 (individually applies "Schiff-base intermediate with DNA")
    • Subsequence at position 3 - 3 aligns to "E" in entry P50465 (individually applies "Proton donor")
    • Subsequence at position 53 - 53 aligns to "K" in entry P50465 (individually applies "Proton donor; for beta-elimination activity")
    • Subsequence at position 70 - 70 aligns to "Q" in entry P50465 (individually applies "DNA")
    • Subsequence at position 125 - 125 aligns to "R" in entry P50465 (individually applies "DNA")
    • Subsequence at position 169 - 169 aligns to "N" in entry P50465 (individually applies "DNA")
    • Subsequence at position 253 - 253 aligns to "R" in entry P50465 (individually applies "Proton donor; for delta-elimination activity")

... then these annotations are applied i

Protein namei

  • Recommended name:
    Endonuclease 8
    Alternative name(s):
    DNA glycosylase/AP lyase Nei (EC:3.2.2.-, EC:4.2.99.18)
    Endonuclease VIII
    DNA-(apurinic or apyrimidinic site) lyase Nei

Gene namei

  • Name:nei

Catalytic activityi

  • The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Functioni

  • Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.

Cofactori

  • Zn2+Note: Binds 1 zinc ion per subunit.

Sequence similaritiesi

Initiator methioninei

  • Removed (to residues corresponding to position 1)

Active sitei

  • Schiff-base intermediate with DNA (to residues corresponding to position 2)
  • Proton donor (to residues corresponding to position 3)
  • Proton donor; for beta-elimination activity (to residues corresponding to position 53)
  • Proton donor; for delta-elimination activity (to residues corresponding to position 253)

Binding sitei

  • DNA (to residues corresponding to position 70)
  • DNA (to residues corresponding to position 125)
  • DNA (to residues corresponding to position 169)

Keywordsi

GO (Gene Ontology) termsi

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