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The annotation and conditions in this rule are derived from the following entries: P08622 (DNAJ_ECOLI)

If a protein meets these conditions... i

Common conditions

Special conditions

    • Subsequence at position 144 - 144 aligns to "C" in entry P08622 Subsequence at position 147 - 147 aligns to "C" in entry P08622 Subsequence at position 197 - 197 aligns to "C" in entry P08622 Subsequence at position 200 - 200 aligns to "C" in entry P08622
    • Subsequence at position 161 - 161 aligns to "C" in entry P08622 Subsequence at position 164 - 164 aligns to "C" in entry P08622 Subsequence at position 183 - 183 aligns to "C" in entry P08622 Subsequence at position 186 - 186 aligns to "C" in entry P08622
  • Subsequence at position 144 - 144 aligns to "C" in entry P08622 (applies "Zinc 1") Subsequence at position 147 - 147 aligns to "C" in entry P08622 (applies "Zinc 1") Subsequence at position 197 - 197 aligns to "C" in entry P08622 (applies "Zinc 1") Subsequence at position 200 - 200 aligns to "C" in entry P08622 (applies "Zinc 1")
  • Subsequence at position 144 - 151 aligns to "C-x(2)-C-x-G-x-G" in entry P08622 (applies "CXXCXGXG motif") Subsequence at position 161 - 168 aligns to "C-x(2)-C-x-G-x-G" in entry P08622 (applies "CXXCXGXG motif") Subsequence at position 183 - 190 aligns to "C-x(2)-C-x-G-x-G" in entry P08622 (applies "CXXCXGXG motif") Subsequence at position 197 - 204 aligns to "C-x(2)-C-x-G-x-G" in entry P08622 (applies "CXXCXGXG motif")
    • Subsequence at position 77 - 114 aligns to entry P08622
  • Subsequence at position 161 - 161 aligns to "C" in entry P08622 (applies "Zinc 2") Subsequence at position 164 - 164 aligns to "C" in entry P08622 (applies "Zinc 2") Subsequence at position 183 - 183 aligns to "C" in entry P08622 (applies "Zinc 2") Subsequence at position 186 - 186 aligns to "C" in entry P08622 (applies "Zinc 2")

... then these annotations are applied i

Protein namei

  • Recommended name:
    Chaperone protein DnaJ

Gene namei

  • Name:dnaJ

Domaini

  • The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.

Subunit structurei

  • Homodimer.

Functioni

  • Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.

Cofactori

  • Zn2+Note: Binds 2 Zn2+ ions per monomer.

Subcellular locationi

Sequence similaritiesi

Metal bindingi

  • Zinc 1 (to residues corresponding to position 144)
  • Zinc 1 (to residues corresponding to position 147)
  • Zinc 1 (to residues corresponding to position 197)
  • Zinc 1 (to residues corresponding to position 200)
  • Zinc 2 (to residues corresponding to position 161)
  • Zinc 2 (to residues corresponding to position 164)
  • Zinc 2 (to residues corresponding to position 183)
  • Zinc 2 (to residues corresponding to position 186)

Repeati

  • CXXCXGXG motif (to residues corresponding to positions 144 - 151)
  • CXXCXGXG motif (to residues corresponding to positions 161 - 168)
  • CXXCXGXG motif (to residues corresponding to positions 183 - 190)
  • CXXCXGXG motif (to residues corresponding to positions 197 - 204)

Compositional biasi

  • Gly-rich (to residues corresponding to positions 77 - 114)

Keywordsi

GO (Gene Ontology) termsi