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If a protein meets these conditions... i

Common conditions

    • Matches HAMAP signature MF_02002
    • taxon = Bacteria
    • fragment ≠ the sequence is fragmented

Special conditions

    • Subsequence at position 886 - 886 aligns to "C" in entry P41972 Subsequence at position 889 - 889 aligns to "C" in entry P41972 Subsequence at position 906 - 906 aligns to "C" in entry P41972 Subsequence at position 909 - 909 aligns to "C" in entry P41972
  • Subsequence at position 886 - 886 aligns to "C" in entry P41972 (applies "Zinc") Subsequence at position 889 - 889 aligns to "C" in entry P41972 (applies "Zinc") Subsequence at position 906 - 906 aligns to "C" in entry P41972 (applies "Zinc") Subsequence at position 909 - 909 aligns to "C" in entry P41972 (applies "Zinc")
    • Subsequence at position 57 - 67 aligns to entry P41972 (individually applies ""HIGH" region")
    • Subsequence at position 554 - 554 aligns to "E" in entry P41972 (individually applies "Aminoacyl-adenylate")
    • Subsequence at position 595 - 599 aligns to entry P41972 (individually applies ""KMSKS" region")
    • Subsequence at position 598 - 598 aligns to "K" in entry P41972 (individually applies "ATP")

... then these annotations are applied i

Protein namei

  • Recommended name:
    Isoleucine--tRNA ligase (EC:6.1.1.5)
    Alternative name(s):
    Isoleucyl-tRNA synthetase
    Short name:
    IleRS

Gene namei

  • Name:ileS

Cofactori

  • Zn2+Note: Binds 1 zinc ion per subunit.

Catalytic activityi

  • ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Functioni

  • Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

Domaini

  • IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).

Subcellular locationi

Sequence similaritiesi

Subunit structurei

  • Monomer.

Metal bindingi

  • Zinc (to residues corresponding to position 886)
  • Zinc (to residues corresponding to position 889)
  • Zinc (to residues corresponding to position 906)
  • Zinc (to residues corresponding to position 909)

Modified residuei

  • N6-acetyllysine (to residues corresponding to position 183)

Binding sitei

  • Aminoacyl-adenylate (to residues corresponding to position 554)
  • ATP (to residues corresponding to position 598)

Motifi

  • "HIGH" region (to residues corresponding to positions 57 - 67)
  • "KMSKS" region (to residues corresponding to positions 595 - 599)

Keywordsi

GO (Gene Ontology) termsi