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The annotation and conditions in this rule are derived from the following entries: O31618 (THIG_BACSU), P30139 (THIG_ECOLI)

If a protein meets these conditions... i

Common conditions

    • Matches HAMAP signature MF_00443
    • taxon = Bacteria
    • fragment ≠ the sequence is fragmented
    • Matches HAMAP signature MF_00443
    • gene location = Plastid
    • fragment ≠ the sequence is fragmented

Special conditions

    • gene location = Plastid; Chloroplast
    • gene location ≠ Plastid; Chloroplast
    • Subsequence at position 98 - 98 aligns to "K" in entry O31618 (individually applies "Schiff-base intermediate with DXP")
    • Subsequence at position 159 - 159 aligns to "G" in entry O31618 (individually applies "DXP; via amide nitrogen")
    • Subsequence at position 185 - 186 aligns to "A-G" in entry O31618 (individually applies "DXP binding")
    • Subsequence at position 207 - 208 aligns to "x-[TS]" in entry O31618 (individually applies "DXP binding")

... then these annotations are applied i

Protein namei

  • Recommended name:
    Thiazole synthase (EC:2.8.1.10)

Gene namei

  • Name:thiG

Subunit structurei

  • Homotetramer. Forms heterodimers with either ThiH or ThiS.

Pathwayi

  • Pathwayi: thiamine diphosphate biosynthesis

    This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Functioni

  • Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.

Catalytic activityi

  • 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.

Subcellular locationi

Sequence similaritiesi

Regioni

  • DXP binding (to residues corresponding to positions 185 - 186)
  • DXP binding (to residues corresponding to positions 207 - 208)

Active sitei

  • Schiff-base intermediate with DXP (to residues corresponding to position 98)

Binding sitei

  • DXP; via amide nitrogen (to residues corresponding to position 159)

Keywordsi

GO (Gene Ontology) termsi