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If a protein meets these conditions... i

Common conditions

Special conditions

    • Subsequence at position 53 - 53 aligns to "C" in entry P12996 (individually applies "Iron-sulfur 1 (4Fe-4S-S-AdoMet)")
    • Subsequence at position 57 - 57 aligns to "C" in entry P12996 (individually applies "Iron-sulfur 1 (4Fe-4S-S-AdoMet)")
    • Subsequence at position 60 - 60 aligns to "C" in entry P12996 (individually applies "Iron-sulfur 1 (4Fe-4S-S-AdoMet)")
    • Subsequence at position 97 - 97 aligns to "[CS]" in entry P12996 (individually applies "Iron-sulfur 2 (2Fe-2S)")
    • Subsequence at position 128 - 128 aligns to "[CS]" in entry P12996 (individually applies "Iron-sulfur 2 (2Fe-2S)")
    • Subsequence at position 188 - 188 aligns to "C" in entry P12996 (individually applies "Iron-sulfur 2 (2Fe-2S)")
    • Subsequence at position 260 - 260 aligns to "[KR]" in entry P12996 (individually applies "Iron-sulfur 2 (2Fe-2S)")

... then these annotations are applied i

Protein namei

  • Recommended name:
    Biotin synthase (EC:2.8.1.6)

Gene namei

  • Name:bioB

Catalytic activityi

  • Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.

Cofactori

  • [2Fe-2S] clusterNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.
  • [4Fe-4S] clusterNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Functioni

  • Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.

Pathwayi

  • Pathwayi: biotin biosynthesis

    This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate. This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Subunit structurei

  • Homodimer.

Sequence similaritiesi

Metal bindingi

  • Iron-sulfur 1 (4Fe-4S-S-AdoMet) (to residues corresponding to position 53)
  • Iron-sulfur 1 (4Fe-4S-S-AdoMet) (to residues corresponding to position 57)
  • Iron-sulfur 1 (4Fe-4S-S-AdoMet) (to residues corresponding to position 60)
  • Iron-sulfur 2 (2Fe-2S) (to residues corresponding to position 97)
  • Iron-sulfur 2 (2Fe-2S) (to residues corresponding to position 128)
  • Iron-sulfur 2 (2Fe-2S) (to residues corresponding to position 188)
  • Iron-sulfur 2 (2Fe-2S) (to residues corresponding to position 260)

Keywordsi

GO (Gene Ontology) termsi