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If a protein meets these conditions... i

Common conditions

Special conditions

    • Subsequence at position 53 - 53 aligns to "C" in entry P12996 (individually applies "Iron-sulfur 1 (4Fe-4S-S-AdoMet)")
    • Subsequence at position 57 - 57 aligns to "C" in entry P12996 (individually applies "Iron-sulfur 1 (4Fe-4S-S-AdoMet)")
    • Subsequence at position 60 - 60 aligns to "C" in entry P12996 (individually applies "Iron-sulfur 1 (4Fe-4S-S-AdoMet)")
    • Subsequence at position 97 - 97 aligns to "[CS]" in entry P12996 (individually applies "Iron-sulfur 2 (2Fe-2S)")
    • Subsequence at position 128 - 128 aligns to "[CS]" in entry P12996 (individually applies "Iron-sulfur 2 (2Fe-2S)")
    • Subsequence at position 188 - 188 aligns to "C" in entry P12996 (individually applies "Iron-sulfur 2 (2Fe-2S)")
    • Subsequence at position 260 - 260 aligns to "[KR]" in entry P12996 (individually applies "Iron-sulfur 2 (2Fe-2S)")

... then these annotations are applied i

Protein namei

  • Recommended name:
    Biotin synthase (EC:2.8.1.6)

Gene namei

  • Name:bioB

Subunit structurei

  • Homodimer.

Pathwayi

  • Pathwayi: biotin biosynthesis

    This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate. This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.

Cofactori

  • [2Fe-2S] clusterNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.
  • [4Fe-4S] clusterNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Functioni

  • Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.

Sequence similaritiesi

Catalytic activityi

  • Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.

Metal bindingi

  • Iron-sulfur 1 (4Fe-4S-S-AdoMet) (to residues corresponding to position 53)
  • Iron-sulfur 1 (4Fe-4S-S-AdoMet) (to residues corresponding to position 57)
  • Iron-sulfur 1 (4Fe-4S-S-AdoMet) (to residues corresponding to position 60)
  • Iron-sulfur 2 (2Fe-2S) (to residues corresponding to position 97)
  • Iron-sulfur 2 (2Fe-2S) (to residues corresponding to position 128)
  • Iron-sulfur 2 (2Fe-2S) (to residues corresponding to position 188)
  • Iron-sulfur 2 (2Fe-2S) (to residues corresponding to position 260)

Keywordsi

GO (Gene Ontology) termsi