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If a protein meets these conditions... i

Common conditions

Special conditions

    • Subsequence at position 128 - 128 aligns to "S" in entry P25924
    • Subsequence at position 22 - 23 aligns to entry P25924 (individually applies "NAD")
    • Subsequence at position 43 - 44 aligns to entry P25924 (individually applies "NAD")
    • Subsequence at position 216 - @CTER|+1@ aligns to entry P25924 (individually applies "Uroporphyrinogen-III C-methyltransferase")
    • Subsequence at position 225 - 225 aligns to "P" in entry P25924 (individually applies "S-adenosyl-L-methionine; via carbonyl oxygen")
    • Subsequence at position 248 - 248 aligns to "D" in entry P25924 (individually applies "Proton acceptor")
    • Subsequence at position 270 - 270 aligns to "K" in entry P25924 (individually applies "Proton donor")
    • Subsequence at position 301 - 303 aligns to "G-G-D" in entry P25924 (individually applies "S-adenosyl-L-methionine binding")
    • Subsequence at position 306 - 306 aligns to "[IVL]" in entry P25924 (individually applies "S-adenosyl-L-methionine; via carbonyl oxygen")
    • Subsequence at position 331 - 332 aligns to "[TS]-A" in entry P25924 (individually applies "S-adenosyl-L-methionine binding")
    • Subsequence at position 382 - 382 aligns to "M" in entry P25924 (individually applies "S-adenosyl-L-methionine; via amide nitrogen")
    • Subsequence at position 411 - 411 aligns to "[GA]" in entry P25924 (individually applies "S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen")
    • Subsequence at position @NTER|+1@ - 204 aligns to entry P25924 (individually applies "precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase")
    • Subsequence at position 128 - 128 aligns to "S" in entry P25924

... then these annotations are applied i

Protein namei

  • Recommended name:
    Siroheme synthase

Cleaved chain(s) or included domain(s)i

  • Includes domain:
    Recommended name:
    Uroporphyrinogen-III C-methyltransferase (EC:2.1.1.107)
    Short name:
    Urogen III methylase
    Alternative name(s):
    Uroporphyrinogen III methylase
    Short name:
    UROM
    SUMT
  • Includes domain:
    Recommended name:
    Sirohydrochlorin ferrochelatase (EC:4.99.1.4)
  • Includes domain:
    Recommended name:
    Precorrin-2 dehydrogenase (EC:1.3.1.76)

Gene namei

  • Name:cysG

Pathwayi

  • Pathwayi: adenosylcobalamin biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III. This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
  • Pathwayi: adenosylcobalamin biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2. This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
  • Pathwayi: siroheme biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III. This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
  • Pathwayi: siroheme biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes siroheme from sirohydrochlorin. This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
  • Pathwayi: siroheme biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2. This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.

Functioni

  • Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.

Sequence similaritiesi

Catalytic activityi

  • Precorrin-2 + NAD+ = sirohydrochlorin + NADH.
  • S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.
  • S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.
  • Siroheme + 2 H+ = sirohydrochlorin + Fe2+.

Regioni

  • Uroporphyrinogen-III C-methyltransferase (to residues corresponding to positions 216 - @CTER|+1@i)
  • S-adenosyl-L-methionine binding (to residues corresponding to positions 301 - 303)
  • S-adenosyl-L-methionine binding (to residues corresponding to positions 331 - 332)
  • precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase (to residues corresponding to positions @NTER|+1@i - 204)

Modified residuei

  • Phosphoserine (to residues corresponding to position 128)

Active sitei

  • Proton acceptor (to residues corresponding to position 248)
  • Proton donor (to residues corresponding to position 270)

Nucleotide bindingi

  • NAD (to residues corresponding to positions 22 - 23)
  • NAD (to residues corresponding to positions 43 - 44)

Binding sitei

  • S-adenosyl-L-methionine; via carbonyl oxygen (to residues corresponding to position 225)
  • S-adenosyl-L-methionine; via carbonyl oxygen (to residues corresponding to position 306)
  • S-adenosyl-L-methionine; via amide nitrogen (to residues corresponding to position 382)
  • S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen (to residues corresponding to position 411)

Keywordsi

GO (Gene Ontology) termsi