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If a protein meets these conditions... i

Common conditions

Special conditions

    • Subsequence at position 68 - 68 aligns to "C" in entry P60716 (individually applies "Iron-sulfur 1 (4Fe-4S)")
    • Subsequence at position 73 - 73 aligns to "C" in entry P60716 (individually applies "Iron-sulfur 1 (4Fe-4S)")
    • Subsequence at position 79 - 79 aligns to "C" in entry P60716 (individually applies "Iron-sulfur 1 (4Fe-4S)")
    • Subsequence at position 94 - 94 aligns to "C" in entry P60716 (individually applies "Iron-sulfur 2 (4Fe-4S-S-AdoMet)")
    • Subsequence at position 98 - 98 aligns to "C" in entry P60716 (individually applies "Iron-sulfur 2 (4Fe-4S-S-AdoMet)")
    • Subsequence at position 101 - 101 aligns to "C" in entry P60716 (individually applies "Iron-sulfur 2 (4Fe-4S-S-AdoMet)")

... then these annotations are applied i

Protein namesi

  • Recommended name:
    Probable lipoyl synthase (EC:2.8.1.8)
    Alternative name(s):
    Lipoic acid synthase
    Lipoate synthase
    Sulfur insertion protein LipA
    Lip-syn
    Short name:
    LS
  • Recommended name:
    Lipoyl synthase (EC:2.8.1.8)
    Alternative name(s):
    Lipoic acid synthase
    Lipoate synthase
    Lip-syn
    Short name:
    LS
    Sulfur insertion protein LipA

Gene namei

  • Name:lipA

Sequence similaritiesi

Pathwayi

  • Pathwayi: protein lipoylation via endogenous pathway

    This protein is involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]. This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
    View all proteins of this organism that are known to be involved in the pathway protein lipoylation via endogenous pathway and in Protein modification.
  • Pathwayi: protein lipoylation via endogenous pathway

    This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]. This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Catalytic activityi

  • Protein N6-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N6-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.

Subcellular locationi

Functioni

  • Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.

Cofactori

  • [4Fe-4S] clusterNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Metal bindingi

  • Iron-sulfur 1 (4Fe-4S) (to residues corresponding to position 68)
  • Iron-sulfur 1 (4Fe-4S) (to residues corresponding to position 73)
  • Iron-sulfur 1 (4Fe-4S) (to residues corresponding to position 79)
  • Iron-sulfur 2 (4Fe-4S-S-AdoMet) (to residues corresponding to position 94)
  • Iron-sulfur 2 (4Fe-4S-S-AdoMet) (to residues corresponding to position 98)
  • Iron-sulfur 2 (4Fe-4S-S-AdoMet) (to residues corresponding to position 101)

Keywordsi

GO (Gene Ontology) termsi