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If a protein meets these conditions... i

Common conditions

Special conditions

    • Subsequence at position 68 - 68 aligns to "C" in entry P60716 (individually applies "Iron-sulfur 1 (4Fe-4S)")
    • Subsequence at position 73 - 73 aligns to "C" in entry P60716 (individually applies "Iron-sulfur 1 (4Fe-4S)")
    • Subsequence at position 79 - 79 aligns to "C" in entry P60716 (individually applies "Iron-sulfur 1 (4Fe-4S)")
    • Subsequence at position 94 - 94 aligns to "C" in entry P60716 (individually applies "Iron-sulfur 2 (4Fe-4S-S-AdoMet)")
    • Subsequence at position 98 - 98 aligns to "C" in entry P60716 (individually applies "Iron-sulfur 2 (4Fe-4S-S-AdoMet)")
    • Subsequence at position 101 - 101 aligns to "C" in entry P60716 (individually applies "Iron-sulfur 2 (4Fe-4S-S-AdoMet)")

... then these annotations are applied i

Protein namesi

  • Recommended name:
    Probable lipoyl synthase (EC:2.8.1.8)
    Alternative name(s):
    Sulfur insertion protein LipA
    Lip-syn
    Short name:
    LS
    Lipoic acid synthase
    Lipoate synthase
  • Recommended name:
    Lipoyl synthase (EC:2.8.1.8)
    Alternative name(s):
    Sulfur insertion protein LipA
    Lip-syn
    Short name:
    LS
    Lipoic acid synthase
    Lipoate synthase

Gene namei

  • Name:lipA

Pathwayi

  • Pathwayi: protein lipoylation via endogenous pathway

    This protein is involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]. This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
  • Pathwayi: protein lipoylation via endogenous pathway

    This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]. This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.

Cofactori

  • [4Fe-4S] clusterNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Sequence similaritiesi

Subcellular locationi

Functioni

  • Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.

Catalytic activityi

  • Protein N6-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N6-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.

Metal bindingi

  • Iron-sulfur 1 (4Fe-4S) (to residues corresponding to position 68)
  • Iron-sulfur 1 (4Fe-4S) (to residues corresponding to position 73)
  • Iron-sulfur 1 (4Fe-4S) (to residues corresponding to position 79)
  • Iron-sulfur 2 (4Fe-4S-S-AdoMet) (to residues corresponding to position 94)
  • Iron-sulfur 2 (4Fe-4S-S-AdoMet) (to residues corresponding to position 98)
  • Iron-sulfur 2 (4Fe-4S-S-AdoMet) (to residues corresponding to position 101)

Keywordsi

GO (Gene Ontology) termsi