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The annotation and conditions in this rule are derived from the following entries: P21177 (FADB_ECOLI), P28793 (FADB_PSEFR)

If a protein meets these conditions... i

Common conditions

Special conditions

    • Subsequence at position 120 - 120 aligns to "E" in entry P28793 (individually applies "Important for catalytic activity")
    • Subsequence at position 140 - 140 aligns to "E" in entry P28793 (individually applies "Important for catalytic activity")
    • Subsequence at position 297 - 297 aligns to "D" in entry P28793 (individually applies "Substrate")
    • Subsequence at position 312 - @CTER@ aligns to entry P28793 (individually applies "3-hydroxyacyl-CoA dehydrogenase")
    • Subsequence at position 325 - 325 aligns to "M" in entry P28793 (individually applies "NAD; via amide nitrogen")
    • Subsequence at position 344 - 344 aligns to "D" in entry P28793 (individually applies "NAD")
    • Subsequence at position 401 - 403 aligns to "[VI]-[VT]-E" in entry P28793 (individually applies "NAD")
    • Subsequence at position 408 - 408 aligns to "K" in entry P28793 (individually applies "NAD")
    • Subsequence at position 430 - 430 aligns to "S" in entry P28793 (individually applies "NAD")
    • Subsequence at position 451 - 451 aligns to "H" in entry P28793 (individually applies "For 3-hydroxyacyl-CoA dehydrogenase activity")
    • Subsequence at position 454 - 454 aligns to "N" in entry P28793 (individually applies "NAD")
    • Subsequence at position 501 - 501 aligns to "N" in entry P28793 (individually applies "Substrate")
    • Subsequence at position 660 - 660 aligns to "Y" in entry P28793 (individually applies "Substrate")
    • Subsequence at position @NTER@ - 190 aligns to entry P28793 (individually applies "Enoyl-CoA hydratase/isomerase")

... then these annotations are applied i

Protein namei

  • Recommended name:
    Fatty acid oxidation complex subunit alpha

Cleaved chain(s) or included domain(s)i

  • Includes domain:
    Recommended name:
    Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase (EC:4.2.1.17, EC:5.1.2.3, EC:5.3.3.8)
  • Includes domain:
    Recommended name:
    3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35)

Gene namei

  • Name:fadB

Subunit structurei

  • Heterotetramer of two alpha chains (FadB) and two beta chains (FadA).

Catalytic activityi

  • (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.
  • (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.
  • (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.
  • (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.

Pathwayi

  • Pathwayi: fatty acid beta-oxidation

    This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.
    View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sequence similaritiesi

Functioni

  • Involved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate.

Binding sitei

  • Substrate (to residues corresponding to position 297)
  • NAD; via amide nitrogen (to residues corresponding to position 325)
  • NAD (to residues corresponding to position 344)
  • NAD (to residues corresponding to position 408)
  • NAD (to residues corresponding to position 430)
  • NAD (to residues corresponding to position 454)
  • Substrate (to residues corresponding to position 501)
  • Substrate (to residues corresponding to position 660)

Nucleotide bindingi

  • NAD (to residues corresponding to positions 401 - 403)

Sitei

  • Important for catalytic activity (to residues corresponding to position 120)
  • Important for catalytic activity (to residues corresponding to position 140)

Active sitei

  • For 3-hydroxyacyl-CoA dehydrogenase activity (to residues corresponding to position 451)

Regioni

  • 3-hydroxyacyl-CoA dehydrogenase (to residues corresponding to positions 312 - @CTER@i)
  • Enoyl-CoA hydratase/isomerase (to residues corresponding to positions @NTER@i - 190)

Keywordsi

GO (Gene Ontology) termsi