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The annotation and conditions in this rule are derived from the following entries: P0A7Z4 (RPOA_ECOLI), Q925Z2 (RPOA_RHIME), Q9KWU8 (RPOA_THEAQ), Q9Z9H6 (RPOA_THETH)

If a protein meets these conditions... i

Common conditions

    • Matches HAMAP signature MF_00059
    • taxon = Bacteria
    • fragment ≠ the sequence is fragmented
    • Matches HAMAP signature MF_00059
    • gene location = Plastid
    • fragment ≠ the sequence is fragmented

Special conditions

    • gene location = Plastid; Chloroplast
    • gene location ≠ Plastid; Chloroplast
    • Subsequence at position 249 - @CTER@ aligns to entry P0A7Z4 (individually applies "Alpha C-terminal domain (alpha-CTD)")
    • Subsequence at position @NTER@ - 235 aligns to entry P0A7Z4 (individually applies "Alpha N-terminal domain (alpha-NTD)")

... then these annotations are applied i

Protein namesi

  • Recommended name:
    DNA-directed RNA polymerase subunit alpha (EC:2.7.7.6)
    Short name:
    PEP
    Alternative name(s):
    Plastid-encoded RNA polymerase subunit alpha
    Short name:
    RNA polymerase subunit alpha
  • Recommended name:
    DNA-directed RNA polymerase subunit alpha (EC:2.7.7.6)
    Short name:
    RNAP subunit alpha
    Alternative name(s):
    Transcriptase subunit alpha
    RNA polymerase subunit alpha

Gene namei

  • Name:rpoA

Subunit structurei

  • In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
  • In cyanobacteria the RNAP catalytic core is composed of 2 alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
  • Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.

Functioni

  • DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Sequence similaritiesi

Subcellular locationi

Catalytic activityi

  • Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Domaini

  • The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.

Regioni

  • Alpha C-terminal domain (alpha-CTD) (to residues corresponding to positions 249 - @CTER@i)
  • Alpha N-terminal domain (alpha-NTD) (to residues corresponding to positions @NTER@i - 235)

Keywordsi

GO (Gene Ontology) termsi

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