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The annotation and conditions in this rule are derived from the following entries: P04805 (SYE_ECOLI), O25360 (SYE2_HELPY), P27000 (SYE_THET8), Q8DLI5 (SYE_THEEB)

If a protein meets these conditions... i

Common conditions

    • Matches HAMAP signature MF_00022
    • taxon = Bacteria
    • fragment ≠ the sequence is fragmented

Special conditions

    • Subsequence at position 98 - 98 aligns to "C" in entry P04805 Subsequence at position 100 - 100 aligns to "C" in entry P04805 Subsequence at position 125 - 125 aligns to "[CH]" in entry P04805 Subsequence at position 127 - 127 aligns to "[HED]" in entry P04805
  • Subsequence at position 98 - 98 aligns to "C" in entry P04805 (applies "Zinc") Subsequence at position 100 - 100 aligns to "C" in entry P04805 (applies "Zinc") Subsequence at position 125 - 125 aligns to "[CH]" in entry P04805 (applies "Zinc") Subsequence at position 127 - 127 aligns to "[HED]" in entry P04805 (applies "Zinc")
    • Subsequence at position 9 - 19 aligns to "P-[SN]-P-x(4)-[HR]-[LIVMAT]-[GE]-x" in entry P04805 (individually applies ""HIGH" region")
    • Subsequence at position 237 - 241 aligns to "x-[LIMSF]-S-K-[KR]" in entry P04805 (individually applies ""KMSKS" region")
    • Subsequence at position 240 - 240 aligns to "K" in entry P04805 (individually applies "ATP")

... then these annotations are applied i

Protein namei

  • Recommended name:
    Glutamate--tRNA ligase (EC:6.1.1.17)
    Alternative name(s):
    Glutamyl-tRNA synthetase
    Short name:
    GluRS

Gene namei

  • Name:gltX

Functioni

  • Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).

Cofactori

  • Zn2+Note: Binds 1 zinc ion per subunit.

Subunit structurei

  • Monomer.

Catalytic activityi

  • ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

Sequence similaritiesi

Subcellular locationi

Motifi

  • "HIGH" region (to residues corresponding to positions 9 - 19)
  • "KMSKS" region (to residues corresponding to positions 237 - 241)

Binding sitei

  • ATP (to residues corresponding to position 240)

Metal bindingi

  • Zinc (to residues corresponding to position 98)
  • Zinc (to residues corresponding to position 100)
  • Zinc (to residues corresponding to position 125)
  • Zinc (to residues corresponding to position 127)

Keywordsi

GO (Gene Ontology) termsi