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If a protein meets these conditions... i

Common conditions

Special conditions

    • Matches PROSITE signature PS50206 Subsequence at position 344 - 456 aligns to "C-x*-C" in entry P77718
    • Does not match PROSITE signature PS50206
    • Matches PROSITE signature PS50206
    • Subsequence at position 344 - 456 aligns to "C-x*-C" in entry P77718
    • Matches PROSITE signature PS50206
    • Subsequence at position 456 - 456 aligns to "C" in entry P77718
    • Subsequence at position 183 - 184 aligns to "[LM]-x" in entry Q81KU0 (individually applies "ATP")
    • Subsequence at position 208 - 209 aligns to "[HYNTS]-[FY]" in entry Q81KU0 (individually applies "ATP")
    • Subsequence at position 265 - 265 aligns to "[RK]" in entry Q81KU0 (individually applies "ATP")
    • Subsequence at position 287 - 287 aligns to "G" in entry Q81KU0 (individually applies "ATP; via amide nitrogen")
    • Subsequence at position 296 - 296 aligns to "Q" in entry Q81KU0 (individually applies "ATP")

... then these annotations are applied i

Protein namesi

  • Recommended name:
    tRNA sulfurtransferase (EC:2.8.1.4)
    Alternative name(s):
    Sulfur carrier protein ThiS sulfurtransferase
    Thiamine biosynthesis protein ThiI
    tRNA 4-thiouridine synthase
  • Recommended name:
    Probable tRNA sulfurtransferase (EC:2.8.1.4)
    Alternative name(s):
    Sulfur carrier protein ThiS sulfurtransferase
    tRNA 4-thiouridine synthase
    Thiamine biosynthesis protein ThiI

Gene namei

  • Name:thiI

Pathwayi

  • Pathwayi: thiamine diphosphate biosynthesis

    This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Subcellular locationi

Sequence similaritiesi

Functioni

  • Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.

Catalytic activityi

  • ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster.
  • [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.

Disulfide bondi

  • Redox-active (to residues corresponding to positions 344 - 456)

Nucleotide bindingi

  • ATP (to residues corresponding to positions 183 - 184)
  • ATP (to residues corresponding to positions 208 - 209)

Active sitei

  • Cysteine persulfide intermediate (to residues corresponding to position 456)

Binding sitei

  • ATP (to residues corresponding to position 265)
  • ATP; via amide nitrogen (to residues corresponding to position 287)
  • ATP (to residues corresponding to position 296)

Keywordsi

GO (Gene Ontology) termsi