Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

If a protein meets these conditions... i

Common conditions

Special conditions

    • Matches PROSITE signature PS50206 Subsequence at position 344 - 456 aligns to "C-x*-C" in entry P77718
    • Does not match PROSITE signature PS50206
    • Matches PROSITE signature PS50206
    • Subsequence at position 344 - 456 aligns to "C-x*-C" in entry P77718
    • Matches PROSITE signature PS50206
    • Subsequence at position 456 - 456 aligns to "C" in entry P77718
    • Subsequence at position 183 - 184 aligns to "[LM]-x" in entry Q81KU0 (individually applies "ATP")
    • Subsequence at position 208 - 209 aligns to "[HYNTS]-[FY]" in entry Q81KU0 (individually applies "ATP")
    • Subsequence at position 265 - 265 aligns to "[RK]" in entry Q81KU0 (individually applies "ATP")
    • Subsequence at position 287 - 287 aligns to "G" in entry Q81KU0 (individually applies "ATP; via amide nitrogen")
    • Subsequence at position 296 - 296 aligns to "Q" in entry Q81KU0 (individually applies "ATP")

... then these annotations are applied i

Protein namesi

  • Recommended name:
    tRNA sulfurtransferase (EC:2.8.1.4)
    Alternative name(s):
    Thiamine biosynthesis protein ThiI
    tRNA 4-thiouridine synthase
    Sulfur carrier protein ThiS sulfurtransferase
  • Recommended name:
    Probable tRNA sulfurtransferase (EC:2.8.1.4)
    Alternative name(s):
    Thiamine biosynthesis protein ThiI
    tRNA 4-thiouridine synthase
    Sulfur carrier protein ThiS sulfurtransferase

Gene namei

  • Name:thiI

Pathwayi

  • Pathwayi: thiamine diphosphate biosynthesis

    This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.

Sequence similaritiesi

Functioni

  • Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.

Catalytic activityi

  • ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster.
  • [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.

Subcellular locationi

Nucleotide bindingi

  • ATP (to residues corresponding to positions 183 - 184)
  • ATP (to residues corresponding to positions 208 - 209)

Disulfide bondi

  • Redox-active (to residues corresponding to positions 344 - 456)

Active sitei

  • Cysteine persulfide intermediate (to residues corresponding to position 456)

Binding sitei

  • ATP (to residues corresponding to position 265)
  • ATP; via amide nitrogen (to residues corresponding to position 287)
  • ATP (to residues corresponding to position 296)

Keywordsi

GO (Gene Ontology) termsi