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P49704 (PRP31_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pre-mRNA-processing factor 31
Gene names
Name:PRP31
Ordered Locus Names:YGR091W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length494 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes the association of the U4/U6.U5 tri-snRNP particle with pre-spliceosomes to form the mature spliceosomal complex. Ref.1

Subunit structure

Component of the 25S U4/U6.U5 tri-snRNP particle, a subcomplex of the spliceosome.

Subcellular location

Nucleus.

Domain

Interacts with the snRNP via the Nop domain By similarity.

The coiled coil domain is formed by two non-contiguous helices By similarity.

Miscellaneous

Present with 1260 molecules/cell in log phase SD medium. Ref.9

Sequence similarities

Belongs to the PRP31 family.

Sequence caution

The sequence AAA74984.1 differs from that shown. Reason: Frameshift at positions 361 and 394.

Ontologies

Keywords
   Biological processmRNA processing
mRNA splicing
   Cellular componentNucleus
Spliceosome
   DomainCoiled coil
   LigandRNA-binding
   Molecular functionRibonucleoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processspliceosomal snRNP assembly

Inferred from mutant phenotype Ref.5. Source: SGD

   Cellular componentU4/U6 x U5 tri-snRNP complex

Inferred from direct assay Ref.7Ref.6. Source: SGD

mitochondrion

Inferred from direct assay. Source: SGD

spliceosomal complex

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 494494Pre-mRNA-processing factor 31
PRO_0000058589

Regions

Coiled coil88 – 12235 By similarity
Coiled coil191 – 22535 By similarity

Sites

Site2571Interaction with U4 snRNA By similarity

Experimental info

Sequence conflict311L → F in AAU09731. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P49704 [UniParc].

Last modified February 21, 2006. Version 2.
Checksum: E39AC6247859FB3B

FASTA49456,303
        10         20         30         40         50         60 
MSSEEDYFDE LEYDLADEVN EEKEDIQTKK LTTVNCQTEK FNPFEILPES IELFRTLALI 

        70         80         90        100        110        120 
SPDRLSLSET AQILPKIVDL KRILQQQEID FIKLLPFFNE IIPLIKSNIK LMHNFLISLY 

       130        140        150        160        170        180 
SRRFPELSSL IPSPLQYSKV ISILENENYS KNESDELFFH LENKAKLTRE QILVLTMSMK 

       190        200        210        220        230        240 
TSFKNKEPLD IKTRTQILEA NSILENLWKL QEDIGQYIAS KISIIAPNVC FLVGPEIAAQ 

       250        260        270        280        290        300 
LIAHAGGVLE FSRIPSCNIA SIGKNKHLSH ELHTLESGVR QEGYLFASDM IQKFPVSVHK 

       310        320        330        340        350        360 
QMLRMLCAKV SLAARVDAGQ KNGDRNTVLA HKWKAELSKK ARKLSEAPSI SETKALPIPE 

       370        380        390        400        410        420 
DQPKKKRAGR KFRKYKEKFR LSHVRQLQNR MEFGKQEQTV LDSYGEEVGL GMSNTSLQQA 

       430        440        450        460        470        480 
VGATSGSRRS AGNQAKLTKV MKHRISEANQ QADEFLISLG HNTEQPNLSP EMVQMHKKQH 

       490 
TNPEEETNWF SGHG

« Hide

References

« Hide 'large scale' references
[1]"The PRP31 gene encodes a novel protein required for pre-mRNA splicing in Saccharomyces cerevisiae."
Weidenhammer E.M., Singh M., Ruiz-Noriega M., Woolford J.L. Jr.
Nucleic Acids Res. 24:1164-1170(1996) [PubMed: 8604353] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed: 9169869] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Prp31p promotes the association of the U4/U6.U5 tri-snRNP with prespliceosomes to form spliceosomes in Saccharomyces cerevisiae."
Weidenhammer E.M., Ruiz-Noriega M., Woolford J.L. Jr.
Mol. Cell. Biol. 17:3580-3588(1997) [PubMed: 9199293] [Abstract]
Cited for: IDENTIFICATION IN U4/U6.U5 TRI-SNRNP COMPLEX.
[6]"Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP."
Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R., Fabrizio P.
EMBO J. 18:4535-4548(1999) [PubMed: 10449419] [Abstract]
Cited for: IDENTIFICATION IN U4/U6.U5 TRI-SNRNP COMPLEX, MASS SPECTROMETRY.
[7]"Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins."
Stevens S.W., Abelson J.
Proc. Natl. Acad. Sci. U.S.A. 96:7226-7231(1999) [PubMed: 10377396] [Abstract]
Cited for: IDENTIFICATION IN U4/U6.U5 TRI-SNRNP COMPLEX, MASS SPECTROMETRY.
[8]"Composition and functional characterization of the yeast spliceosomal penta-snRNP."
Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J.
Mol. Cell 9:31-44(2002) [PubMed: 11804584] [Abstract]
Cited for: IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX, MASS SPECTROMETRY.
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U31970 Genomic DNA. Translation: AAA74984.1. Frameshift.
Z72876 Genomic DNA. Translation: CAA97094.1.
AY723814 Genomic DNA. Translation: AAU09731.1.
BK006941 Genomic DNA. Translation: DAA08184.1.
PIRS64386.
RefSeqNP_011605.1. NM_001181220.1.

3D structure databases

ProteinModelPortalP49704.
SMRP49704. Positions 87-346.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-5355N.
IntActP49704. 34 interactions.
MINTMINT-493013.
STRINGP49704.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR091W; YGR091W; YGR091W.
GeneID852983.
KEGGsce:YGR091W.
NMPDRfig|4932.3.peg.2722.

Organism-specific databases

CYGDYGR091w.
SGDS000003323. PRP31.

Phylogenomic databases

eggNOGfuNOG09129.
GeneTreeEFGT00050000000030.
OMAHRISEAN.
OrthoDBEOG4ZCXDV.

Gene expression databases

ArrayExpressP49704.
GenevestigatorP49704.
GermOnlineYGR091W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR012976. NOSIC.
IPR019175. Prp31_C.
IPR002687. SnoRNA-bd_dom.
[Graphical view]
KOK12844.
PfamPF01798. Nop. 1 hit.
PF08060. NOSIC. 1 hit.
PF09785. Prp31_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972794.

Entry information

Entry namePRP31_YEAST
AccessionPrimary (citable) accession number: P49704
Secondary accession number(s): D6VUM3, Q66R99
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: February 21, 2006
Last modified: December 14, 2011
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents