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Protein

Siroheme synthase

Gene

cysG

Organism
Neisseria meningitidis
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.UniRule annotationSAAS annotation

Catalytic activityi

2 S-adenosyl-L-methionine + uroporphyrinogen III = 2 S-adenosyl-L-homocysteine + precorrin-2.UniRule annotationSAAS annotation
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotationSAAS annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotationSAAS annotation

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cobA), Siroheme synthase (cysG), Siroheme synthase (cobA), Siroheme synthase (cobA), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cysG)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cobA), Siroheme synthase (cysG), Siroheme synthase (cobA), Siroheme synthase (cobA), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cysG)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes siroheme from sirohydrochlorin.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cobA), Siroheme synthase (cysG), Siroheme synthase (cobA), Siroheme synthase (cobA), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cysG)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes siroheme from sirohydrochlorin, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cobA), Siroheme synthase (cysG), Siroheme synthase (cobA), Siroheme synthase (cobA), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cysG)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei226S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei249Proton acceptorUniRule annotation1
Active sitei271Proton donorUniRule annotation1
Binding sitei307S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei384S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei413S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi22 – 23NADUniRule annotation2
Nucleotide bindingi43 – 44NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotation, MethyltransferaseUniRule annotationSAAS annotationImported, Multifunctional enzymeUniRule annotationSAAS annotation, OxidoreductaseUniRule annotationSAAS annotationImported, Transferase
Biological processCobalamin biosynthesisUniRule annotationSAAS annotation, Porphyrin biosynthesisUniRule annotationSAAS annotation
LigandNADUniRule annotationSAAS annotation, S-adenosyl-L-methionineUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00148; UER00222
UPA00262; UER00211
UPA00262; UER00222
UPA00262; UER00376

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthaseUniRule annotation
Including the following 3 domains:
Sirohydrochlorin ferrochelataseUniRule annotation (EC:4.99.1.4UniRule annotation)
Precorrin-2 dehydrogenaseUniRule annotation (EC:1.3.1.76UniRule annotation)
Uroporphyrinogen-III C-methyltransferaseUniRule annotation (EC:2.1.1.107UniRule annotation)
Short name:
Urogen III methylaseUniRule annotation
Alternative name(s):
SUMTUniRule annotation
Uroporphyrinogen III methylaseUniRule annotation
Short name:
UROMUniRule annotation
Gene namesi
Name:cysGUniRule annotationImported
ORF Names:ERS040961_00152Imported, NMA510612_1516Imported
OrganismiNeisseria meningitidisImported
Taxonomic identifieri487 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
Proteomesi
  • UP000046839 Componenti: Unassembled WGS sequence
  • UP000023582 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei128PhosphoserineUniRule annotation1

Keywords - PTMi

PhosphoproteinUniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini119 – 145Sirohm_synth_MInterPro annotationAdd BLAST27
Domaini150 – 206CysG_dimeriserInterPro annotationAdd BLAST57
Domaini219 – 428TP_methylaseInterPro annotationAdd BLAST210

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 203precorrin-2 dehydrogenase / sirohydrochlorin ferrochelataseUniRule annotationAdd BLAST203
Regioni217 – 486Uroporphyrinogen-III C-methyltransferaseUniRule annotationAdd BLAST270
Regioni302 – 304S-adenosyl-L-methionine bindingUniRule annotation3
Regioni332 – 333S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the precorrin methyltransferase family.UniRule annotation
In the C-terminal section; belongs to the precorrin methyltransferase family.UniRule annotationSAAS annotation
In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CB8 Bacteria
COG0007 LUCA
COG1648 LUCA
KOiK02302

Family and domain databases

CDDicd11642 SUMT, 1 hit
Gene3Di1.10.8.210, 1 hit
3.30.950.10, 1 hit
3.40.1010.10, 1 hit
HAMAPiMF_01646 Siroheme_synth, 1 hit
InterProiView protein in InterPro
IPR000878 4pyrrol_Mease
IPR035996 4pyrrol_Methylase_sf
IPR014777 4pyrrole_Mease_sub1
IPR014776 4pyrrole_Mease_sub2
IPR006366 CobA/CysG_C
IPR036291 NAD(P)-bd_dom_sf
IPR037115 Sirohaem_synt_dimer_dom_sf
IPR012409 Sirohaem_synth
IPR028281 Sirohaem_synthase_central
IPR019478 Sirohaem_synthase_dimer_dom
IPR006367 Sirohaem_synthase_N
IPR003043 Uropor_MeTrfase_CS
PfamiView protein in Pfam
PF10414 CysG_dimeriser, 1 hit
PF14824 Sirohm_synth_M, 1 hit
PF00590 TP_methylase, 1 hit
PIRSFiPIRSF036426 Sirohaem_synth, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF53790 SSF53790, 1 hit
TIGRFAMsiTIGR01469 cobA_cysG_Cterm, 1 hit
TIGR01470 cysG_Nterm, 1 hit
PROSITEiView protein in PROSITE
PS00839 SUMT_1, 1 hit
PS00840 SUMT_2, 1 hit

Sequencei

Sequence statusi: Complete.

X5EQR7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYFPIFANL AGRPVLVVGG GAVAARKISL LLKAGAEVRV AAKHLNAELS
60 70 80 90 100
ALAAENKILW LAEEFRAEHI RTVFLIIAAS SDQALNRRVF HLAESCQKPV
110 120 130 140 150
NVVDDRDHCS FIFPSVIDRN PVQIAVSSSG SAPVLARLLR ERLEALLPPS
160 170 180 190 200
LGDMAEISGR WRDAVKGKLK SVTERRRFWE KQFNGRFAAL VKNRQNTLAE
210 220 230 240 250
RELAGQLEQS RQNDQGGSVS LVGAGPGDAG LLTLKGLQEI QQADVVLYDA
260 270 280 290 300
LVSDGILSLV RRDAERIFVG KRARGGRTPQ EDTNALMVRL AREGRRVVRL
310 320 330 340 350
KGGDPFVFGR GGEELETLAR HQIPFSVVPG ITAAVGATAY AGIPLTHRDY
360 370 380 390 400
AQSAVFVTGH RKADAPDIEW QTLARSRQTL VIYMGALKAA LIAERLQQHG
410 420 430 440 450
RSPDTPAAVI SQGTLPAQKT ATGTLANLAE LAETAPNPAL IVIGEVVGLH
460 470 480
EKLAWFGENA KKESNPAEHA YFALDGLGTG QEQQAA
Length:486
Mass (Da):52,484
Last modified:June 11, 2014 - v1
Checksum:iF70D6A5F50FF23CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP007524 Genomic DNA Translation: AHW75806.1
CFME01000002 Genomic DNA Translation: CFC38530.1
RefSeqiWP_002246198.1, NZ_CP012694.1

Genome annotation databases

EnsemblBacteriaiAHW75806; AHW75806; NMA510612_1516
CFC38530; CFC38530; ERS040961_00152
KEGGinmx:NMA510612_1516
PATRICifig|487.1203.peg.671

Similar proteinsi

Entry informationi

Entry nameiX5EQR7_NEIME
AccessioniPrimary (citable) accession number: X5EQR7
Entry historyiIntegrated into UniProtKB/TrEMBL: June 11, 2014
Last sequence update: June 11, 2014
Last modified: May 23, 2018
This is version 40 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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