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Protein

D-alanine--D-alanyl carrier protein ligase

Gene

dltA

Organism
Staphylococcus aureus
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the first step in the D-alanylation of lipoteichoic acid (LTA), the activation of D-alanine and its transfer onto the D-alanyl carrier protein (Dcp) DltC. In an ATP-dependent two-step reaction, forms a high energy D-alanyl-AMP intermediate, followed by transfer of the D-alanyl residue as a thiol ester to the phosphopantheinyl prosthetic group of the Dcp. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall.UniRule annotationSAAS annotation

Catalytic activityi

D-alanine + ATP + holo-[D-alanyl-carrier protein] = AMP + diphosphate + D-alanyl-[D-alanyl-carrier protein].UniRule annotationSAAS annotation

Pathwayi: lipoteichoic acid biosynthesis

This protein is involved in the pathway lipoteichoic acid biosynthesis, which is part of Cell wall biogenesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway lipoteichoic acid biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei189D-alanineUniRule annotation1
Binding sitei293D-alanine; via carbonyl oxygenUniRule annotation1
Binding sitei365ATPUniRule annotation1
Binding sitei473ATPUniRule annotation1
Binding sitei473D-alanineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi144 – 145ATPUniRule annotation2
Nucleotide bindingi284 – 289ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationSAAS annotationImported
LigandATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00556.

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanyl carrier protein ligaseUniRule annotation (EC:6.2.1.-UniRule annotation)
Short name:
DCLUniRule annotation
Alternative name(s):
D-alanine--poly(phosphoribitol) ligase subunit 1UniRule annotation
D-alanine-activating enzymeUniRule annotation
Short name:
DAEUniRule annotation
Gene namesi
Name:dltAUniRule annotationImported
ORF Names:B9Z08_03700Imported, EQ90_04305Imported, ERS072738_00109Imported, HMPREF3211_01980Imported
OrganismiStaphylococcus aureusImported
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000071995 Componenti: Unassembled WGS sequence
  • UP000070177 Componenti: Unassembled WGS sequence
  • UP000229567 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 386AMP-bindingInterPro annotationAdd BLAST378
Domaini395 – 473AMP-binding_CInterPro annotationAdd BLAST79

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family. DltA subfamily.UniRule annotationSAAS annotation

Phylogenomic databases

KOiK03367.

Family and domain databases

HAMAPiMF_00593. DltA. 1 hit.
InterProiView protein in InterPro
IPR010071. AA_adenyl_domain.
IPR025110. AMP-bd_C.
IPR000873. AMP-dep_Synth/Lig.
IPR010072. DltA.
PfamiView protein in Pfam
PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
TIGRFAMsiTIGR01733. AA-adenyl-dom. 1 hit.
TIGR01734. D-ala-DACP-lig. 1 hit.

Sequencei

Sequence statusi: Complete.

X5EMW7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDIINKLQA FADANPQSIA VRHTTDELTY QQLMDESSKL AHRLQGSKKP
60 70 80 90 100
MILFGHMSPY MIVGMIGAIK AGCGYVPVDT SIPEDRIKMI INKVQPEFVF
110 120 130 140 150
NTTDESFESL EGEVFTIEDI KTSQDPVIFD SQIKDNDTVY TIFTSGSTGE
160 170 180 190 200
PKGVQIEYAS LVQFTEWMLE LNKSGNEQQW LNQAPFSFDL SVMAIYPCLA
210 220 230 240 250
SGGTLNLVDK NMINKPKLLN EMLTATPINI WVSTPSFMEM CLLLPTLNEE
260 270 280 290 300
QYGSLNEFFF CGEILPHRAA KALVNRFPSA TIYNTYGPTE ATVAVTSIQI
310 320 330 340 350
TQEILDQYPT LPVGVERPGA RLSTTDEGEL VIEGQSVSLG YLKNDQKTAE
360 370 380 390 400
VFNFDDGIRT YHTGDKAKFE NGQWFIQGRI DFQIKLNGYR MELEEIETQL
410 420 430 440 450
RQSEFVKEAI VVPVYKNDKV IHLIGAIVPT TEVTDNAEMT KNIKNDLKSR
460 470 480
LPEYMIPRKF EWMEQLPLTS NGKIDRKKIA EVING
Length:485
Mass (Da):54,670
Last modified:June 11, 2014 - v1
Checksum:iFFA535607F1FD328
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FGBR01000001 Genomic DNA. Translation: CXL46552.1.
LALJ01000006 Genomic DNA. Translation: KMR37401.1.
LRQH01000146 Genomic DNA. Translation: KXA34243.1.
NESW01000002 Genomic DNA. Translation: PHZ48736.1.
RefSeqiWP_000129653.1. NZ_PGGL01000001.1.

Genome annotation databases

EnsemblBacteriaiCFF98890; CFF98890; ERS093009_00357.
CXE18884; CXE18884; ERS074020_00104.
CXL46552; CXL46552; ERS072738_00109.
CZQ25806; CZQ25806; ERS1058648_00233.
KXA34243; KXA34243; HMPREF3211_01980.
OAP80407; OAP80407; A4U86_00805.
SCR11086; SCR11086; SAMEA2298760_00779.
KEGGisauf:X998_0910.
PATRICifig|1280.10758.peg.803.

Similar proteinsi

Entry informationi

Entry nameiX5EMW7_STAAU
AccessioniPrimary (citable) accession number: X5EMW7
Entry historyiIntegrated into UniProtKB/TrEMBL: June 11, 2014
Last sequence update: June 11, 2014
Last modified: March 28, 2018
This is version 43 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteomeImported