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Protein

Hydroxyacylglutathione hydrolase

Gene

gloB

Organism
Candidatus Sodalis pierantonius str. SOPE
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.UniRule annotationSAAS annotation

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.UniRule annotationSAAS annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Pathwayi: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Hydroxyacylglutathione hydrolase (gloB)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi53Zinc 1UniRule annotation1
Metal bindingi55Zinc 1UniRule annotation1
Metal bindingi57Zinc 2UniRule annotation1
Metal bindingi58Zinc 2UniRule annotation1
Metal bindingi109Zinc 1UniRule annotation1
Metal bindingi126Zinc 1UniRule annotation1
Metal bindingi126Zinc 2UniRule annotation1
Metal bindingi164Zinc 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotationImported
LigandMetal-bindingUniRule annotationSAAS annotation, ZincUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00619; UER00676.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolaseUniRule annotation (EC:3.1.2.6UniRule annotation)
Alternative name(s):
Glyoxalase IIUniRule annotation
Short name:
Glx IIUniRule annotation
Gene namesi
Name:gloBUniRule annotationImported
ORF Names:SOPEG_0762Imported
OrganismiCandidatus Sodalis pierantonius str. SOPEImported
Taxonomic identifieri2342 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesPectobacteriaceaeSodalis
Proteomesi
  • UP000019025 Componenti: Chromosome

Interactioni

Subunit structurei

Monomer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 164Lactamase_BInterPro annotationAdd BLAST154

Sequence similaritiesi

Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.UniRule annotationSAAS annotation

Phylogenomic databases

KOiK01069.

Family and domain databases

CDDicd07723. hydroxyacylglutathione_hydrola. 1 hit.
Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiView protein in InterPro
IPR035680. Clx_II_MBL.
IPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
IPR036866. RibonucZ/Hydroxyglut_hydro.
PfamiView protein in Pfam
PF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
PIRSFiPIRSF005457. Glx. 1 hit.
SMARTiView protein in SMART
SM00849. Lactamase_B. 1 hit.
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.

Sequencei

Sequence statusi: Complete.

W0HMB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLISIPALA DNYIWLLHND DSQCLVVDPG EAAPVLQALA DRHLIPVAIL
60 70 80 90 100
LTHHHHDHVG GVAELLRHFP VPVYGPEETR AKGSTRIVNE GDTLTLLGHT
110 120 130 140 150
FSIMALPGHT LGHIGFYGAP WLFSGDTVFS AGCGRLFEGT PKQMYASFQK
160 170 180 190 200
VNQLPPDTLI CAAHEYTSSN LDFAAALLPQ DSVITGYQRE IKELRLKNQP
210 220 230 240 250
SLPTTLHLER QINLFLRCHD IDLQNKLNAH PASGEEWRVF AALREKKDHF
Length:250
Mass (Da):27,786
Last modified:March 19, 2014 - v1
Checksum:i762F78303F235DC9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP006568 Genomic DNA. Translation: AHF73248.1.
RefSeqiWP_025244392.1. NZ_CP006568.1.

Genome annotation databases

EnsemblBacteriaiAHF73248; AHF73248; SOPEG_0762.
KEGGipes:SOPEG_0762.

Similar proteinsi

Entry informationi

Entry nameiW0HMB5_9GAMM
AccessioniPrimary (citable) accession number: W0HMB5
Entry historyiIntegrated into UniProtKB/TrEMBL: March 19, 2014
Last sequence update: March 19, 2014
Last modified: February 28, 2018
This is version 31 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported