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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Sphingomonas sanxanigenens DSM 19645 = NX02
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotationSAAS annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotation, NucleotidyltransferaseUniRule annotationSAAS annotationImported, Transferase
LigandMagnesiumUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
ORF Names:NX02_23165Imported
OrganismiSphingomonas sanxanigenens DSM 19645 = NX02Imported
Taxonomic identifieri1123269 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingomonas
Proteomesi
  • UP000018851 Componenti: Chromosome

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini728 – 805ACTInterPro annotationAdd BLAST78
Domaini839 – 914ACTInterPro annotationAdd BLAST76

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 374UridylyltransferaseUniRule annotationAdd BLAST374

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili787 – 811Sequence analysisAdd BLAST25

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotationSAAS annotation

Keywords - Domaini

Coiled coilSequence analysis, RepeatSAAS annotation

Phylogenomic databases

KOiK00990.

Family and domain databases

HAMAPiMF_00277. PII_uridylyl_transf. 1 hit.
InterProiView protein in InterPro
IPR002912. ACT_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
IPR010043. UTase/UR.
PANTHERiPTHR13734:SF45. PTHR13734:SF45. 2 hits.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiView protein in SMART
SM00471. HDc. 1 hit.
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiView protein in PROSITE
PS51671. ACT. 2 hits.

Sequencei

Sequence statusi: Complete.

W0AEC5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNRFDALPN RRAIIDRRVL ADAIAALDPK VGDPALRAAV AGLLRQALAD
60 70 80 90 100
GRAEIAKRLD EHPSRGLEAA SGQAFLIDQL LRLIFDFVTH SLYPINNPTA
110 120 130 140 150
GERIVLVAVG GYGRGEMAPF SDVDIAFLTP WKQTGWTEQV IESMLYLLWD
160 170 180 190 200
LGLKVGHSSR SLDEMVRMAK ADLTIRTALL EGRYVWGDQA LYDQAAERFD
210 220 230 240 250
REVMAGTART FVAEKLAERD ARHRKMGDSR YVVEPNVKEG KGGLRDLHTL
260 270 280 290 300
FWIGKYVHSV RSVAELVDAG LLTREELRRF QRAENFLWAV RCHLHALSGR
310 320 330 340 350
GEDRLTFDVQ REIAVKMRYA DRPGKSAVER FMQHYFLTAK QVGDVTGLFL
360 370 380 390 400
AHLDEQHAAR GRRFGLPAIM RRAPKKLEGF VLDRGRLALP SEDFFQEDPV
410 420 430 440 450
RLIQIFALAD EQGLEIHPLA MRAASRDARR IDNGVRRDRR ANAFFMDVLT
460 470 480 490 500
SPRDPETVLR WMNEASVFGR FVPDFGRVVA QMQFDMYHHY TVDEHTVRAI
510 520 530 540 550
GLLSKIEKGA LETDHPLATA IMDQIVSRRV LYVAVLLHDI AKGRAGDHSV
560 570 580 590 600
LGAEVALKLC PRFGMTPAET ETVAWLVRRH LLMSATAFKR DLSDFKTVLD
610 620 630 640 650
FVQAVQSPER LRLLLVLTVV DIRAVGPGVW NGWKRQLLSD LYDAAEEVLR
660 670 680 690 700
LGHKQRGRGE RVVAKQAELR RLLGWTDEAF DALVRRLPES YWIAESVDVL
710 720 730 740 750
ERNAKQVAEA DVPLSIAAQV YPARDATLVT VYASDHPGLF YRIAGAIHLA
760 770 780 790 800
GASIIDARIH TTRDGMALDN FLVQDQRGGP FDGEDRLQRI VRTIEDALAN
810 820 830 840 850
RNRLNERLQA RPLAKIRAEA FSIEPVVLID NKASNRYTVI EVNARDRPAL
860 870 880 890 900
LNALAYALFQ SKVTIHSAHI ATYGERAVDT FYITDLIGDK IDGALRLQAL
910
ETRLLDAAAG RESELEAA
Length:918
Mass (Da):103,139
Last modified:March 19, 2014 - v1
Checksum:i7C7C1FC8CE5B4904
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP006644 Genomic DNA. Translation: AHE56249.1.
RefSeqiWP_025294372.1. NZ_CP006644.1.

Genome annotation databases

EnsemblBacteriaiAHE56249; AHE56249; NX02_23165.
KEGGissan:NX02_23165.
PATRICifig|1123269.5.peg.4533.

Similar proteinsi

Entry informationi

Entry nameiW0AEC5_9SPHN
AccessioniPrimary (citable) accession number: W0AEC5
Entry historyiIntegrated into UniProtKB/TrEMBL: March 19, 2014
Last sequence update: March 19, 2014
Last modified: November 22, 2017
This is version 32 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteome, Multifunctional enzymeUniRule annotationSAAS annotation, Reference proteomeImported