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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Sulfolobus acidocaldarius SUSAZ
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei597ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetaseUniRule annotation, Ligase
Biological processProtein biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, ZincUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
ORF Names:SUSAZ_02715Imported
OrganismiSulfolobus acidocaldarius SUSAZImported
Taxonomic identifieri1435377 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000060777 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 632tRNA-synt_1InterPro annotationAdd BLAST619
Domaini681 – 829Anticodon_1InterPro annotationAdd BLAST149

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi594 – 598"KMSKS" regionUniRule annotation5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

KOiK01870.
OrthoDBiPOG093Z0054.

Family and domain databases

CDDicd07961. Anticodon_Ia_Ile_ABEc. 1 hit.
Gene3Di3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2. 1 hit.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033709. Anticodon_Ile_ABEc.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
PfamiView protein in Pfam
PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

V9S4E5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSKFDPLKF EEEVLKYWDD NKIYKKLKEI NEKNHKKFLF IDGPPYPSSP
60 70 80 90 100
IPHIGTVWNK TIKDCILRYE RLMGYSVKDQ PGYDTHGLPI EVETEKRLGI
110 120 130 140 150
KSKAEIIEKI GVDNFISKCK EFAINNSKSL TQNFRNLGIF MDWENPYFTF
160 170 180 190 200
NNDYISNSWA VIKKAYERGL LYKGVHVLHW CSRCETTLAD YEVSEYRDLE
210 220 230 240 250
DPSIYVKFRV KGEPNRYLVI WTTTPWTLPA NVFVMVNKDF EYADVRVGDE
260 270 280 290 300
ILVIAKDRVK ELMKEARIKE YKILRVYKGE ELIGLEYEHP LADIVNAQSK
310 320 330 340 350
INNHHKVLDG GEAVTLQEGT GLVHSAPGHG DIDFEIGKKY DMPVVMLVND
360 370 380 390 400
KGEFTQDSGK YAGKYVRSAS EEIISDLKQR NALVYASKIV HRYPVCWRCK
410 420 430 440 450
TPLILRAIEQ WFIAVSKLKD NLMGEIDRVR WIPDWGKTRI GNMVKEVRDW
460 470 480 490 500
VISRQRFWGI PLPIWVCSNC ENIIVVGGVD ELNKISINQV PQDLHRPWID
510 520 530 540 550
SVVVRCEKCG GEARRISDVA DVWFDSGVAF FASLGKDWRK KWSELGPVDL
560 570 580 590 600
VLEGHDQLRG WFFSLLRTGV ILMDRAPYEA VLVHGFMLDE QGKEMHKSSG
610 620 630 640 650
NYVEPSQVVS KYGRDTLRLW LLRNTTWEDA KFSWKSLDMT RRDLNIIWNV
660 670 680 690 700
YVFANTYMSL DEFKYSKYSY QDIKDYLKLE DIWLLSRYYR MLKEVTEAMK
710 720 730 740 750
EYKVHELANK VTTFIIDDIS RFYLRVTRKR AWNEANDPDK IAMYYVLYHV
760 770 780 790 800
LKGSLILLST VIPFAAEKIY LDFVQERLES ISMEKIPEIK EEFINEEIEE
810 820 830 840 850
AFEIAKEIID AGLNARAKAG IKLRWPLKEV YVFLVSDKDR RSIEKITDVL
860 870 880 890 900
SSLLNSKSII IEGIDGYKRF SKIRATPNTG SIGPTFKRLS VKVAEYIQNN
910 920 930 940 950
SDKVAQDIVS KGYHEFNVDS ENLRLDISHV NLVEEVEKGY ASARFSKGVV
960 970 980 990 1000
LLKQEMSKEE EEEGIIRDLI RRIQFMRKQL SLNVNEYILL SIRAPNDKVD
1010 1020 1030 1040 1050
LIKKWEQYIK NETRAKELRI GDVSGDLIQD WDVEEETYTI GVSKADVSVS
Length:1,050
Mass (Da):122,150
Last modified:March 19, 2014 - v1
Checksum:i934C561BD65469CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP006977 Genomic DNA. Translation: AHC51005.1.

Genome annotation databases

EnsemblBacteriaiAHC51005; AHC51005; SUSAZ_02715.
KEGGisacs:SUSAZ_02715.

Similar proteinsi

Entry informationi

Entry nameiV9S4E5_9CREN
AccessioniPrimary (citable) accession number: V9S4E5
Entry historyiIntegrated into UniProtKB/TrEMBL: March 19, 2014
Last sequence update: March 19, 2014
Last modified: March 28, 2018
This is version 30 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteomeImported