gid

Functionⁱ

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotationSAAS annotation

Catalytic activityⁱ

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH₂ = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotationSAAS annotation

Cofactorⁱ

FADUniRule annotationSAAS annotation

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	32 – 37	FADUniRule annotation		6

GO - Molecular functionⁱ

5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity Source: UniProtKB-UniRule
flavin adenine dinucleotide binding Source: UniProtKB-UniRule
methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Source: UniProtKB-EC

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	MethyltransferaseUniRule annotationSAAS annotationImported, Transferase
Biological process	tRNA processingUniRule annotationSAAS annotation
Ligand	FADUniRule annotationSAAS annotation, Flavoprotein

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotationSAAS annotation (EC:2.1.1.74UniRule annotationSAAS annotation) Alternative name(s): Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesⁱ	Name:gidImported Synonyms:trmFOUniRule annotation ORF Names:U758_06870Imported
Organismⁱ	Streptococcus mitis 27/7Imported
Taxonomic identifierⁱ	1415766 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Terrabacteria group › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus mitis
Proteomesⁱ	UP000018726 Componentⁱ: Unassembled WGS sequence

Subcellular locationⁱ

Cytoplasm
UniRule annotation
Automatic assertion according to rulesⁱ
- HAMAP-Rule:MF_01037
SAAS annotation
Automatic assertion according to rulesⁱ
- SAAS:SAAS00919417

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

CytoplasmUniRule annotationSAAS annotation

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the MnmG family. TrmFO subfamily.UniRule annotationSAAS annotation

Family and domain databases

Gene3Dⁱ	3.50.50.60. 2 hits.
HAMAPⁱ	MF_01037. TrmFO. 1 hit.
InterProⁱ	View protein in InterPro IPR036188. FAD/NAD-bd_sf. IPR002218. MnmG-rel. IPR020595. MnmG-rel_CS. IPR004417. TrmFO.
PANTHERⁱ	PTHR11806. PTHR11806. 1 hit. PTHR11806:SF2. PTHR11806:SF2. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01134. GIDA. 1 hit.
SUPFAMⁱ	SSF51905. SSF51905. 1 hit.
TIGRFAMsⁱ	TIGR00137. gid_trmFO. 1 hit.
PROSITEⁱ	View protein in PROSITE PS01281. GIDA_2. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

V8I9Y9-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MLNKPVPCFD MNLGSTQRGI SVVSQSYINV IGAGLAGSEA AYQIAERGIP 
        60         70         80         90        100
VKLYEMRGVK STPQHKTDNF AELVCSNSLR GDALTNAVGL LKEEMRRLGS 
       110        120        130        140        150
VILESAEATR VPAGGALAVD RDGFSQMVTE KVANHPLIEV VRDEITELPT 
       160        170        180        190        200
DVITVVATGP LTSDALAEKI HSLNDGDGFY FYDAAAPIID VNTIDMSKVY 
       210        220        230        240        250
LKSRYDKGEA AYLNAPMTKQ EFMDFHEALV NAEEAPLNSF EKEKYFEGCM 
       260        270        280        290        300
PIEVMAKRGI KTMLYGPMKP VGLEYPDDYT GPRDGEFKTP YAVVQLRQDN 
       310        320        330        340        350
AAGSLYNIVG FQTHLKWGEQ KRVFQMIPGL ENAEFVRYGV MHRNSYMDSP 
       360        370        380        390        400
NLLEQTYRSK KQPNLFFAGQ MTGVEGYVES AASGLVAGIN AARLFKGESE 
       410        420        430        440        450
AIFPETTAIG SLAHYITHAD SKHFQPMNVN FGIIKELEGE RIRDKKARYE 
       460 
KIAERALSDL EEFLTV

Length:466

Mass (Da):51,563

Last modified:February 19, 2014 - v1

Checksum:ⁱ40807A12113A340E

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AYRS01000012 Genomic DNA. Translation: ETD97879.1.

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

AYRS01000012 Genomic DNA. Translation: ETD97879.1.

Genome annotation databases

EnsemblBacteriaⁱ	ETD97879; ETD97879; U758_06870.
PATRICⁱ	fig\|1415766.3.peg.1322.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
V8I9Y9	W1UPS1 W1UKU2 UPI0009B7F2DA	Streptococcus sp. DORA_10 Streptococcus mitis	466	UniRef100_V8I9Y9	Cluster: Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO	4

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
V8I9Y9	Q97R84 B2IP98 W1UPS1 W1UKU2 UPI0009B7F2DA D4FSA8 A0A139PJK9 E7SBB8 UPI00021BCC09 I1ZLY2 +540	Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) Streptococcus pneumoniae (strain CGSP14) Streptococcus sp. DORA_10 Streptococcus mitis Streptococcus oralis ATCC 35037 Streptococcus oralis Streptococcus australis ATCC 700641 Streptococcus australis Streptococcus parasanguinis FW213 Streptococcus parasanguinis And more	444	UniRef90_Q97R84	Cluster: Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO	551

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
V8I9Y9	P39815 A0A162TX84 A0A1B2B3Z8 A0A125UHV7 L8AJP5 A0A0T8PQD7 UPI00033082FC A0A132BHS8 A0A2A2VVI3 UPI000A10C985 +5323	Bacillus subtilis (strain 168) Bacillus subtilis Bacillus subtilis subsp. subtilis Bacillus sp. LM 4-2 Bacillus subtilis BEST7613 Streptococcus pneumoniae Bacillus atrophaeus Bacillus halotolerans Bacillus sp. 7705b Bacillus And more	435	UniRef50_P39815	Cluster: Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO	5,334

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AYRS01000012 Genomic DNA. Translation: ETD97879.1.

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

AYRS01000012 Genomic DNA. Translation: ETD97879.1.

3D structure databases

ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	ETD97879; ETD97879; U758_06870.
PATRICⁱ	fig\|1415766.3.peg.1322.

Family and domain databases

Gene3Dⁱ	3.50.50.60. 2 hits.
HAMAPⁱ	MF_01037. TrmFO. 1 hit.
InterProⁱ	View protein in InterPro IPR036188. FAD/NAD-bd_sf. IPR002218. MnmG-rel. IPR020595. MnmG-rel_CS. IPR004417. TrmFO.
PANTHERⁱ	PTHR11806. PTHR11806. 1 hit. PTHR11806:SF2. PTHR11806:SF2. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01134. GIDA. 1 hit.
SUPFAMⁱ	SSF51905. SSF51905. 1 hit.
TIGRFAMsⁱ	TIGR00137. gid_trmFO. 1 hit.
PROSITEⁱ	View protein in PROSITE PS01281. GIDA_2. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	V8I9Y9_STRMT
Accessionⁱ	V8I9Y9Primary (citable) accession number: V8I9Y9
Entry historyⁱ	Integrated into UniProtKB/TrEMBL:	February 19, 2014
	Last sequence update:	February 19, 2014
	Last modified:	February 28, 2018
	This is version 25 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Unreviewed (UniProtKB/TrEMBL)

Miscellaneousⁱ

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termⁱ

Complete proteomeImported

UniProtKB - V8I9Y9 (V8I9Y9_STRMT)

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Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Select a section on the left to see content.

Functionⁱ

Catalytic activityⁱ

Cofactorⁱ

Regions

GO - Molecular functionⁱ

Keywordsⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Family and domain databases

Sequenceⁱ

Sequence databases

Genome annotation databases

Similar proteinsⁱ

Cross-referencesⁱ

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Family and domain databases

Entry informationⁱ

Miscellaneousⁱ

Caution

Keywords - Technical termⁱ

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - V8I9Y9 (V8I9Y9_STRMT)

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Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

gid

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

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Caution

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Functionⁱ

GO - Molecular functionⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ