Reviewed, UniProtKB/Swiss-Prot O75604 (UBP2_HUMAN)

Names and origin

Protein names

Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 2
    EC=3.1.2.15
Alternative name(s):
    Ubiquitin thioesterase 2
    Ubiquitin-specific-processing protease 2
    Deubiquitinating enzyme 2
    41 kDa ubiquitin-specific protease

Gene names

Name:	USP2
Synonyms:	UBP41

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	605 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Ubiquitin C-terminal thioester + H(2)O = ubiquitin + a thiol.
Subunit structure	Homooligomer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the peptidase C19 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase Protease Thiol protease
Technical term	3D-structure
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from direct assay. Source: HPA plasma membrane Inferred from direct assay. Source: HPA
Molecular function	cysteine-type endopeptidase activity Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
KRT15	P19012	1	EBI-743272,EBI-739566

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O75604-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O75604-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-252: Missing.
     253-258: PGRDGM → MLNKAK

Notes: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 605

605

Ubiquitin carboxyl-terminal hydrolase 2

PRO_0000080616

Sites

Active site

276

1

By similarity

Active site

549

1

By similarity

Active site

557

1

By similarity

Natural variations

Alternative sequence

1 – 252

252

Missing in isoform 2.

VSP_005256

Alternative sequence

253 – 258

6

PGRDGM → MLNKAK in isoform 2.

VSP_005257

Experimental info

Sequence conflict

421

1

S → G in BAB71388. Ref.2

Sequence conflict

501

1

H → R in BAB71388. Ref.2

Sequence conflict

594

1

L → H in AAC28392. Ref.1

Sequence conflict

602 – 605

4

PSRM → TSPI in AAC28392. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

605

Helix Strand Turn

Details...

Helix

276 – 286

11

Helix

289 – 296

8

Helix

299 – 302

4

Helix

313 – 326

14

Helix

337 – 346

10

Helix

348 – 350

3

Helix

358 – 372

15

Helix

392 – 404

13

Helix

410 – 415

6

Beta strand

417 – 425

9

Turn

426 – 428

3

Beta strand

431 – 444

14

Helix

455 – 462

8

Beta strand

466 – 468

3

Helix

470 – 472

3

Turn

477 – 480

4

Beta strand

485 – 493

9

Beta strand

496 – 503

8

Helix

529 – 531

3

Beta strand

532 – 536

5

Beta strand

540 – 551

12

Beta strand

556 – 563

8

Turn

565 – 567

3

Beta strand

570 – 574

5

Beta strand

577 – 581

5

Helix

583 – 585

3

Beta strand

591 – 598

8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 27, 2002. Version 2. Checksum: AFF4DA9344D21812	FASTA	605	68,072
	10 20 30 40 50 60 MSQLSSTLKR YTESARYTDA HYAKSGYGAY TPSSYGANLA ASLLEKEKLG FKPVPTSSFL 70 80 90 100 110 120 TRPRTYGPSS LLDYDRGRPL LRPDITGGGK RAESQTRGTE RPLGSGLSGG SGFPYGVTNN 130 140 150 160 170 180 CLSYLPINAY DQGVTLTQKL DSQSDLARDF SSLRTSDSYR IDPRNLGRSP MLARTRKELC 190 200 210 220 230 240 TLQGLYQTAS CPEYLVDYLE NYGRKGSASQ VPSQAPPSRV PEIISPTYRP IGRYTLWETG 250 260 270 280 290 300 KGQAPGPSRS SSPGRDGMNS KSAQGLAGLR NLGNTCFMNS ILQCLSNTRE LRDYCLQRLY 310 320 330 340 350 360 MRDLHHGSNA HTALVEEFAK LIQTIWTSSP NDVVSPSEFK TQIQRYAPRF VGYNQQDAQE 370 380 390 400 410 420 FLRFLLDGLH NEVNRVTLRP KSNPENLDHL PDDEKGRQMW RKYLEREDSR IGDLFVGQLK 430 440 450 460 470 480 SSLTCTDCGY CSTVFDPFWD LSLPIAKRGY PEVTLMDCMR LFTKEDVLDG DEKPTCCRCR 490 500 510 520 530 540 GRKRCIKKFS IQRFPKILVL HLKRFSESRI RTSKLTTFVN FPLRDLDLRE FASENTNHAV 550 560 570 580 590 600 YNLYAVSNHS GTTMGGHYTA YCRSPGTGEW HTFNDSSVTP MSSSQVRTSD AYLLFYELAS PPSRM
	Isoform 2 [UniParc]. Checksum: 7CDF2F9A300B864F		353	40,638
	10 20 30 40 50 60 MLNKAKNSKS AQGLAGLRNL GNTCFMNSIL QCLSNTRELR DYCLQRLYMR DLHHGSNAHT 70 80 90 100 110 120 ALVEEFAKLI QTIWTSSPND VVSPSEFKTQ IQRYAPRFVG YNQQDAQEFL RFLLDGLHNE 130 140 150 160 170 180 VNRVTLRPKS NPENLDHLPD DEKGRQMWRK YLEREDSRIG DLFVGQLKSS LTCTDCGYCS 190 200 210 220 230 240 TVFDPFWDLS LPIAKRGYPE VTLMDCMRLF TKEDVLDGDE KPTCCRCRGR KRCIKKFSIQ 250 260 270 280 290 300 RFPKILVLHL KRFSESRIRT SKLTTFVNFP LRDLDLREFA SENTNHAVYN LYAVSNHSGT 310 320 330 340 350 TMGGHYTAYC RSPGTGEWHT FNDSSVTPMS SSQVRTSDAY LLFYELASPP SRM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of the human and mouse UBP41." Gong L., Yeh E.T.H. Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Placenta.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lymph.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF079564 mRNA. Translation: AAC28392.1.
AK057225 mRNA. Translation: BAB71388.1.
BC002854 mRNA. Translation: AAH02854.1.
BC002955 mRNA. Translation: AAH02955.1.

RefSeq

NP_004196.4.

UniGene

Hs.524085

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HD5	X-ray	1.85	A	258-605	[»]
2IBI	X-ray	2.20	A	247-605	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:29134N.

IntAct

O75604.

Protein family/group databases

MEROPS

C19.013.

Genome annotation databases

Ensembl

ENSG00000036672. Homo sapiens. [Contig view]

GeneID

9099.

KEGG

hsa:9099.

Organism-specific databases

HGNC

HGNC:12618. USP2.

HPA

HPA006777.
HPA007222.

MIM

604725. gene.

PharmGKB

PA27525.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

O75604.

HOVERGEN

O75604.

Gene expression databases

ArrayExpress

O75604.

CleanEx

HS_USP2.

GermOnline

ENSG00000036672. Homo sapiens.

Family and domain databases

InterPro

IPR001394. Peptidase_C19.
[Graphical view]

Pfam

PF00443. UCH. 1 hit.
[Graphical view]

PROSITE

PS00972. UCH_2_1. 1 hit.
PS00973. UCH_2_2. 1 hit.
PS50235. UCH_2_3. 1 hit.
[Graphical view]

BLOCKS

Search...

ProtoNet

Search...

Other Resources

NextBio

34107.

SOURCE

Search...

Entry information

Entry name

UBP2_HUMAN

Accession

Primary (citable) accession number: O75604
Secondary accession number(s): Q96MB9, Q9BQ21

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	March 27, 2002
Last modified:	November 4, 2008
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)