Ubiquitin-conjugating enzyme E2 D1 - Homo sapiens (Human)

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Reviewed, UniProtKB/Swiss-Prot P51668 (UB2D1_HUMAN)

Last modified November 4, 2008. Version 76. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 D1
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase D1
    Ubiquitin carrier protein D1
    UbcH5
    Ubiquitin-conjugating enzyme E2-17 kDa 1
    E2(17)KB 1
    UBC4/5 homolog
    Stimulator of Fe transport
      Short name=SFT

Gene names

Name:	UBE2D1
Synonyms:	SFT, UBCH5, UBCH5A

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Component of a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X.
Tissue specificity	Ubiquitous. Up-regulated in livers of iron-overloaded patients with hereditary hemochromatosis.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.
Caution	Ref.5 cloned and sequenced SFT which consisted of UBE2D1 last coding exon along with intronic sequences on the 5'-end of this exon. A function in iron transport has been described.

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Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Ligase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	BMP signaling pathway Inferred from Experiment. Source: Reactome anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from Experiment. Source: Reactome negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome positive regulation of protein ubiquitination Inferred from direct assay. Source: UniProtKB positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome protein polyubiquitination Inferred from direct assay. Source: UniProtKB
Cellular component	cytosol Inferred from Experiment. Source: Reactome nucleoplasm Inferred from Experiment. Source: Reactome
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 147

147

Ubiquitin-conjugating enzyme E2 D1

PRO_0000082460

Sites

Active site

Glycyl thioester intermediate By similarity

Secondary structure

147

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P51668-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 2E96FD0179EE119D
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FASTA

147

16,602

        10         20         30         40         50         60 
MALKRIQKEL SDLQRDPPAH CSAGPVGDDL FHWQATIMGP PDSAYQGGVF FLTVHFPTDY 

        70         80         90        100        110        120 
PFKPPKIAFT TKIYHPNINS NGSICLDILR SQWSPALTVS KVLLSICSLL CDPNPDDPLV 

       130        140 
PDIAQIYKSD KEKYNRHARE WTQKYAM

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53." Scheffner M., Huibregtse J.M., Howley P.M. Proc. Natl. Acad. Sci. U.S.A. 91:8797-8801(1994) [PubMed: 8090726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"UbcH5A, a member of human E2 ubiquitin-conjugating enzymes, is closely related to SFT, a stimulator of iron transport, and is up-regulated in hereditary hemochromatosis." Gehrke S.G., Riedel H.-D., Herrmann T., Hadaschik B., Bents K., Veltkamp C., Stremmel W. Blood 101:3288-3293(2003) [PubMed: 12480712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY.
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow and Skin.
[5]	"Functional expression cloning and characterization of SFT, a stimulator of Fe transport." Gutierrez J.A., Yu J., Rivera S., Wessling-Resnick M. J. Cell Biol. 139:895-905(1997) [PubMed: 9362508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 134-147, TISSUE SPECIFICITY.
[6]	Erratum Gutierrez J.A., Yu J., Rivera S., Wessling-Resnick M. J. Cell Biol. 147:205-205(1999)
[7]	"Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses." Matsuzawa S., Reed J.C. Mol. Cell 7:915-926(2001) [PubMed: 11389839] [Abstract] Cited for: SUBUNIT OF A COMPLEX WITH SIAH1; CACYBP; SKP1; APC AND TBL1X.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

X78140 mRNA. Translation: CAA55019.1.
AF257220 mRNA. Translation: AAM81086.1.
AJ272367 mRNA. Translation: CAC82177.1.
AJ293565 Genomic DNA. Translation: CAC82097.1.
BT007041 mRNA. Translation: AAP35690.1.
BC005980 mRNA. Translation: AAH05980.1.
BC015997 mRNA. Translation: AAH15997.1.
AF020761 mRNA. No translation available.

PIR

I39202.

RefSeq

NP_003329.1.

UniGene

Hs.129683

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2C4P	X-ray	2.35	A/B	1-147	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P51668.

Genome annotation databases

Ensembl

ENSG00000072401. Homo sapiens. [Contig view]

GeneID

7321.

KEGG

hsa:7321.

Organism-specific databases

H-InvDB

HIX0008841.

HGNC

HGNC:12474. UBE2D1.

MIM

602961. gene.

PharmGKB

PA37124.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

P51668.

HOVERGEN

P51668.

Enzyme and pathway databases

Reactome

REACT_152. Cell Cycle, Mitotic.
REACT_1538. Cell Cycle Checkpoints.
REACT_6844. Signaling by TGF beta.
REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_8017. APC-Cdc20 mediated degradation of Nek2A.
REACT_9035. APC/C:Cdh1-mediated degradation of Skp2.

Gene expression databases

ArrayExpress

P51668.

CleanEx

HS_UBE2D1.

GermOnline

ENSG00000072401. Homo sapiens.

Family and domain databases

InterPro

IPR016135. UBQ-conjugat/RWD-like.
IPR000608. UBQ-conjugat_E2.
[Graphical view]

Gene3D

G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.

PANTHER

PTHR11621. UBQ-conjugat_E2. 1 hit.

Pfam

PF00179. UQ_con. 1 hit.
[Graphical view]

ProDom

PD000461. UBQ_conjugat. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00212. UBCc. 1 hit.
[Graphical view]

PROSITE

PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

BLOCKS

Search...

ProtoNet

Search...

Other Resources

NextBio

28626.

SOURCE

Search...

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Entry information

Entry name

UB2D1_HUMAN

Accession

Primary (citable) accession number: P51668

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 4, 2008
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Sequence annotation (Features)

Molecule processing

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents