Skip Header

 
Contribute Send feedback

Reviewed, UniProtKB/Swiss-Prot P54578 (UBP14_HUMAN)

Last modified November 4, 2008. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Ubiquitin carboxyl-terminal hydrolase 14
    EC=3.1.2.15
Alternative name(s):
    Ubiquitin thioesterase 14
    Ubiquitin-specific-processing protease 14
    Deubiquitinating enzyme 14
Gene names
Name: USP14
Synonyms: TGT
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Hide | Top

Protein attributes

Sequence length494 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Catalytic activity

Ubiquitin C-terminal thioester + H(2)O = ubiquitin + a thiol.

Subunit structure

Homodimer Potential.

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 ubiquitin-like domain.

Caution

Was originally (Ref.1) thought to be a queuine tRNA-ribosyltransferase.

Hide | Top

Ontologies

Keywords

   Biological processUbl conjugation pathway
   Molecular functionHydrolase
Protease
Thiol protease
   PTMPhosphoprotein
   Technical term3D-structure

Gene Ontology (GO)

   Cellular componentcytoplasm

Inferred from direct assay. Source: HPA

   Molecular functioncysteine-type endopeptidase activity

Traceable author statement. Source: ProtInc

tRNA guanylyltransferase activity

Traceable author statement. Source: ProtInc

ubiquitin-specific protease activity

Traceable author statement. Source: ProtInc

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 494493Ubiquitin carboxyl-terminal hydrolase 14
PRO_0000080636

Regions

Domain4 – 8077Ubiquitin-like

Sites

Active site1141
Active site4261
Active site4351

Amino acid modifications

Modified residue1361Phosphotyrosine By similarity
Modified residue1431Phosphoserine By similarity
Modified residue2221Phosphoserine By similarity

Secondary structure

1 ....................................................... 494
Helix Strand Turn

Details...

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P54578-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: E6D4679A86E9DF00

FASTA49456,069
        10         20         30         40         50         60 
MPLYSVTVKW GKEKFEGVEL NTDEPPMVFK AQLFALTGVQ PARQKVMVKG GTLKDDDWGN 

        70         80         90        100        110        120 
IKIKNGMTLL MMGSADALPE EPSAKTVFVE DMTEEQLASA MELPCGLTNL GNTCYMNATV 

       130        140        150        160        170        180 
QCIRSVPELK DALKRYAGAL RASGEMASAQ YITAALRDLF DSMDKTSSSI PPIILLQFLH 

       190        200        210        220        230        240 
MAFPQFAEKG EQGQYLQQDA NECWIQMMRV LQQKLEAIED DSVKETDSSS ASAATPSKKK 

       250        260        270        280        290        300 
SLIDQFFGVE FETTMKCTES EEEEVTKGKE NQLQLSCFIN QEVKYLFTGL KLRLQEEITK 

       310        320        330        340        350        360 
QSPTLQRNAL YIKSSKISRL PAYLTIQMVR FFYKEKESVN AKVLKDVKFP LMLDMYELCT 

       370        380        390        400        410        420 
PELQEKMVSF RSKFKDLEDK KVNQQPNTSD KKSSPQKEVK YEPFSFADDI GSNNCGYYDL 

       430        440        450        460        470        480 
QAVLTHQGRS SSSGHYVSWV KRKQDEWIKF DDDKVSIVTP EDILRLSGGG DWHIAYVLLY 

       490 
GPRRVEIMEE ESEQ

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"tRNA-guanine transglycosylase cDNA from human placenta."
Deshpande K.L., Katze J.R.
Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Placenta.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Skin.
[3]"Structure and mechanisms of the proteasome-associated deubiquitinating enzyme USP14."
Hu M., Li P., Song L., Jeffrey P.D., Chenova T.A., Wilkinson K.D., Cohen R.E., Shi Y.
EMBO J. 24:3747-3756(2005) [PubMed: 16211010] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 91-494.
+Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

U30888 mRNA. Translation: AAB60365.1.
BC003556 mRNA. Translation: AAH03556.1.
PIRG01932.
RefSeqNP_001032411.1.
NP_005142.1.
UniGeneHs.464416
Hs.702732

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2AYNX-ray3.20A/B/C91-494[»]
2AYOX-ray3.50A91-494[»]
SMRP54578. Positions 6-86.
ModBaseSearch...

Protein family/group databases

MEROPSC19.015.

PTM databases

PhosphoSiteP54578.

2-D gel databases

OGPP54578.

Proteomic databases

PeptideAtlasP54578.

Genome annotation databases

EnsemblENSG00000101557. Homo sapiens. [Contig view]
GeneID9097.
KEGGhsa:9097.

Organism-specific databases

HGNCHGNC:12612. USP14.
HPAHPA001308.
MIM607274. gene.
PharmGKBPA27523.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMP54578.
HOVERGENP54578.

Gene expression databases

ArrayExpressP54578.
CleanExHS_USP14.
GermOnlineENSG00000101557. Homo sapiens.

Family and domain databases

InterProIPR001394. Peptidase_C19.
IPR000626. Ubiquitin.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
SMARTSM00213. UBQ. 1 hit.
[Graphical view]
PROSITEPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
PS00972. UCH_2_1. 1 hit.
PS00973. UCH_2_2. 1 hit.
PS50235. UCH_2_3. 1 hit.
[Graphical view]
BLOCKSSearch...
ProtoNetSearch...

Other Resources

NextBio34093.
SOURCESearch...

Hide | Top

Entry information

Entry nameUBP14_HUMAN
AccessionPrimary (citable) accession number: P54578
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: November 4, 2008
This is version 82 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Hide | Top

Relevant documents

Human chromosome 18

Human chromosome 18: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents