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Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Psychrobacter aquaticus CMS 56
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotationSAAS annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Diaminopimelate epimerase (dapF)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13SubstrateUniRule annotation1
Binding sitei46SubstrateUniRule annotation1
Binding sitei66SubstrateUniRule annotation1
Active sitei75PROSITE-ProRule annotation1
Active sitei75Proton donorUniRule annotation1
Binding sitei162SubstrateUniRule annotation1
Sitei164Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Binding sitei195SubstrateUniRule annotation1
Sitei213Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Active sitei222Proton acceptorUniRule annotation1
Sitei274Important for dimerizationUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomeraseUniRule annotationSAAS annotationImported
Biological processAmino-acid biosynthesis, Lysine biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00034; UER00025

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimeraseUniRule annotationSAAS annotation (EC:5.1.1.7UniRule annotationSAAS annotation)
Short name:
DAP epimeraseUniRule annotation
Alternative name(s):
PLP-independent amino acid racemaseUniRule annotation
Gene namesi
Name:dapFUniRule annotation
ORF Names:M917_1795Imported
OrganismiPsychrobacter aquaticus CMS 56Imported
Taxonomic identifieri1354303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter
Proteomesi
  • UP000016761 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni76 – 77Substrate bindingUniRule annotation2
Regioni213 – 214Substrate bindingUniRule annotation2
Regioni223 – 224Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the diaminopimelate epimerase family.UniRule annotationSAAS annotation

Phylogenomic databases

OrthoDBiPOG091H01QC

Family and domain databases

HAMAPiMF_00197 DAP_epimerase, 1 hit
InterProiView protein in InterPro
IPR018510 DAP_epimerase_AS
IPR001653 DAP_epimerase_DapF
PANTHERiPTHR31689 PTHR31689, 1 hit
PfamiView protein in Pfam
PF01678 DAP_epimerase, 2 hits
TIGRFAMsiTIGR00652 DapF, 1 hit
PROSITEiView protein in PROSITE
PS01326 DAP_EPIMERASE, 1 hit

Sequencei

Sequence statusi: Complete.

U4T524-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIEFTKMHG LGNDFMVIDL VTQRLELTKD LVQLLGDRHL GIGFDQLLVV
60 70 80 90 100
EPPMRPDVDF SYRIFNTDGT EVEQCGNGAR CFARFVQARK LSFKQRLRVE
110 120 130 140 150
TASGIISLTT DRYGWVEVDM GKPKFEPNEI PFTPRATTKI QNAYHLDVNG
160 170 180 190 200
TPVQLYVANM GNPHAVIKVD NVLDAEVETL GKAIESHPAF PDRVNVGFMQ
210 220 230 240 250
VMNQRHIRLR VYERGVGETQ ACGTGACAAV AVGIREGWLD EGEDVRAQLY
260 270 280 290
GGSMIIKWQP GYSVMMTGPT AFVYEGVFSP DGLMAQAGIK TNTED
Length:295
Mass (Da):32,776
Last modified:December 11, 2013 - v1
Checksum:iD9B20F95BE8A985F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AUSW01000032 Genomic DNA Translation: ERL55271.1
RefSeqiWP_021814424.1, NZ_AUSW01000032.1

Genome annotation databases

EnsemblBacteriaiERL55271; ERL55271; M917_1795
GeneIDi33117718
PATRICifig|1354303.4.peg.1763

Similar proteinsi

Entry informationi

Entry nameiU4T524_9GAMM
AccessioniPrimary (citable) accession number: U4T524
Entry historyiIntegrated into UniProtKB/TrEMBL: December 11, 2013
Last sequence update: December 11, 2013
Last modified: April 25, 2018
This is version 26 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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