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Protein

Maternal embryonic leucine zipper kinase

Gene

pig-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase involved in cell autonomous neuroblast asymmetric divisions that generate one precursor cell and one apoptotic cell by controlling spindle positioning, myosin distribution and the segregation of cell fate determinants (PubMed:16774992, PubMed:20929735, PubMed:23946438, PubMed:23267054, PubMed:23851392). Promotes cell shedding during embryogenesis, probably through the endocytosis-mediated removal of cell adhesion molecules such as hmp-1 from the cell surface (PubMed:22801495). May act downstream of par-4/strd-1/mop-25 to regulate cell shedding (PubMed:22801495).6 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40ATPPROSITE-ProRule annotation1
Active sitei132Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 25ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • asymmetric neuroblast division Source: UniProtKB
  • hemidesmosome assembly Source: WormBase
  • intracellular signal transduction Source: GO_Central
  • negative regulation of apoptotic process involved in development Source: UniProtKB
  • neuroblast development Source: UniProtKB
  • peptidyl-tyrosine phosphorylation Source: GO_Central
  • positive regulation of apoptotic process Source: UniProtKB
  • protein phosphorylation Source: WormBase
  • regulation of cell adhesion Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase
Biological processApoptosis, Neurogenesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Maternal embryonic leucine zipper kinaseBy similarity (EC:2.7.11.1By similarity)
Short name:
MELKBy similarity
Gene namesi
Name:pig-1Imported
ORF Names:W03G1.6Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiW03G1.6a ; CE40473 ; WBGene00021012 ; pig-1
W03G1.6b ; CE40474 ; WBGene00021012 ; pig-1
W03G1.6c ; CE48676 ; WBGene00021012 ; pig-1
W03G1.6d ; CE49097 ; WBGene00021012 ; pig-1
W03G1.6e ; CE48957 ; WBGene00021012 ; pig-1
W03G1.6f ; CE48801 ; WBGene00021012 ; pig-1
W03G1.6g ; CE48617 ; WBGene00021012 ; pig-1
W03G1.6h ; CE49061 ; WBGene00021012 ; pig-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

During neuroblast differentiation, mutants produce an additional precursor cell for the HSN/PHB, I2, M4 and PLM/ALN and Q.a and Q.p lineages. The nucleus size of the 2 Q.p daughter cells is similar (PubMed:16774992). Symmetrical distribution of myosin II during QR.a cell division (PubMed:20929735). Double knockouts of pig-1 and ced-3 have impaired cell shedding during embryogenesis which results in the generation of an ectopic excretory cell and an ERM-like neuron. In addition, mutants show a delay in clearance after engulfment of cell corpses which is associated with the abnormal expression of cell adhesion molecules (PubMed:22801495).3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi169T → A: May be inactive. Prevents cell shedding during embryogenesis. Additional production of PVM neuron. 2 Publications1
Mutagenesisi169T → D: May be constitutively active. Promotes cell shedding during embryogenesis. In some mutants, production of an additional PVM neuron. 2 Publications1
Mutagenesisi172G → E in n4780; production of an additional M4 motor neuron. 1 Publication1
Mutagenesisi172G → R in gm300; production of an additional HSN/PHB precursor cell. 1 Publication1
Mutagenesisi198G → D in gm301; production of an additional HSN/PHB precursor cell and an additional PVM precursor cell. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004323871 – 706Maternal embryonic leucine zipper kinaseAdd BLAST706

Post-translational modificationi

May be phosphorylated at Thr-169 by par-4 and/or autophosphorylated which likely results in its activation (PubMed:22801495). Phosphorylation is not required for co-localization with the centrosome (PubMed:23267054).2 Publications

Proteomic databases

PaxDbiU4PR86
PeptideAtlasiU4PR86

Expressioni

Developmental stagei

Ubiquitously expressed in early embryo with a more restricted expression in later embryonic stages and in young larvae. Expressed in dividing cells including ventral nerve cord neuroblasts, vulval precursors, hypodermal seam cells and in the Q lineage. No expression in adults.1 Publication

Gene expression databases

BgeeiWBGene00021012

Interactioni

Protein-protein interaction databases

IntActiU4PR86, 1 interactor
STRINGi6239.W03G1.6a

Structurei

3D structure databases

ProteinModelPortaliU4PR86
SMRiU4PR86
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 265Protein kinasePROSITE-ProRule annotationAdd BLAST255
Domaini656 – 705KA1PROSITE-ProRule annotationAdd BLAST50

Domaini

The KA1 domain is required for the control of asymmetric neuroblast division.1 Publication

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0583 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00920000149073
HOGENOMiHOG000233023
InParanoidiA7WK47
KOiK08799
OMAiYELHETI
OrthoDBiEOG091G05V9

Family and domain databases

CDDicd14078 STKc_MELK, 1 hit
InterProiView protein in InterPro
IPR028375 KA1/Ssp2_C
IPR001772 KA1_dom
IPR011009 Kinase-like_dom_sf
IPR034673 MELK
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF02149 KA1, 1 hit
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF103243 SSF103243, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50032 KA1, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (8)i

Sequence statusi: Complete.

This entry describes 8 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform cImported (identifier: U4PR86-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSKYEVLQGF YAVHDELGSG GFGKVRLATH LLTNQKVAIK IIDKKQLGHD
60 70 80 90 100
LPRVQTEMDA LRNLSHQNIC RLYHYIETED KFFIVMEYCS GGEMFDYIVR
110 120 130 140 150
KERLEESEAR HFFRQLVSAI AFVHSQGYAH RDLKPENLLL TEDLHLKLID
160 170 180 190 200
FGLCAKTEKG RIDKHNLDTC CGSPAYAAPE LIQGLQYKGN EADVWSMGIL
210 220 230 240 250
LYTLLVGALP FEDDNMQIMY KKIQSGCFYE PEFLSPLSKQ LLRAMLQVVP
260 270 280 290 300
ERRISVKKLL EHDWLNHKYT QPVKWNTIYD KNFIDRDVAR VMSKYYGFES
310 320 330 340 350
TDKMIEKIKE WNFDYMTSTY YALLHRKRNG MEIILPMVRN STNTAPPNVQ
360 370 380 390 400
NILCSPTIHA SLENNLDKSG LEDDDSDPSS ISSSSDISAR LKKNCVVSDE
410 420 430 440 450
SSSSRFVKPM SPAAEKDKKM SYVNAMLTMP SQFTGRSPLR IPESPMSVRS
460 470 480 490 500
SDSASLGSAA TPSRGGVKDN DKENASTGKN YRMGASTCKS RGPLKITGVE
510 520 530 540 550
EGTMKSVYTT PNTRPTLRGL FSPGNAEHKK RQRARSSDRA SIGMPPGSPV
560 570 580 590 600
SIGSAHSANN ELLADGRTPR SRIKTNRLPQ RVFTSLERKK EKLITLLTPR
610 620 630 640 650
KMQRDSPQVL KDVKNMVNVS MTASQDPEEV RNLLKKVFDD ERMRYELNGW
660 670 680 690 700
KFLATQETVH GWMTVELEIV RLQMFDKVGI RRKRLKGDAF MYKKVCEKIL

QMAKIE
Note: No experimental confirmation available.Curated
Length:706
Mass (Da):80,340
Last modified:December 11, 2013 - v1
Checksum:i7A6A330E2F5C4E2A
GO
Isoform aImported (identifier: U4PR86-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     561-563: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:703
Mass (Da):79,985
Checksum:iD720707B90C1C046
GO
Isoform bImported (identifier: U4PR86-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     561-563: Missing.
     679-706: GIRRKRLKGDAFMYKKVCEKILQMAKIE → RNLRRFFCCVNINIPSRKPTYIEL

Note: No experimental confirmation available.Curated
Show »
Length:699
Mass (Da):79,584
Checksum:i6E5A53D6688861FA
GO
Isoform dImported (identifier: U4PR86-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     679-706: GIRRKRLKGDAFMYKKVCEKILQMAKIE → RNLRRFFCCVNINIPSRKPTYIEL

Note: No experimental confirmation available.Curated
Show »
Length:702
Mass (Da):79,940
Checksum:iE1C7DDBABB7A5B03
GO
Isoform eImported (identifier: U4PR86-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-244: Missing.
     561-563: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:459
Mass (Da):52,003
Checksum:iD1471995CE4706FB
GO
Isoform fImported (identifier: U4PR86-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-244: Missing.
     561-563: Missing.
     679-706: GIRRKRLKGDAFMYKKVCEKILQMAKIE → RNLRRFFCCVNINIPSRKPTYIEL

Note: No experimental confirmation available.Curated
Show »
Length:455
Mass (Da):51,602
Checksum:iB0DC956B6C561F62
GO
Isoform gImported (identifier: U4PR86-7) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-244: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:462
Mass (Da):52,358
Checksum:iA77B6CF84835A074
GO
Isoform hImported (identifier: U4PR86-8) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-244: Missing.
     679-706: GIRRKRLKGDAFMYKKVCEKILQMAKIE → RNLRRFFCCVNINIPSRKPTYIEL

Note: No experimental confirmation available.Curated
Show »
Length:458
Mass (Da):51,957
Checksum:i46AE33E43DBCE607
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0575061 – 244Missing in isoform e, isoform f, isoform g and isoform h. CuratedAdd BLAST244
Alternative sequenceiVSP_057507561 – 563Missing in isoform a, isoform b, isoform e and isoform f. Curated3
Alternative sequenceiVSP_057508679 – 706GIRRK…MAKIE → RNLRRFFCCVNINIPSRKPT YIEL in isoform b, isoform d, isoform f and isoform h. CuratedAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081096 Genomic DNA Translation: CCD69087.1
FO081096 Genomic DNA Translation: CCD69088.1
FO081096 Genomic DNA Translation: CDH93078.1
FO081096 Genomic DNA Translation: CDH93079.1
FO081096 Genomic DNA Translation: CDH93080.1
FO081096 Genomic DNA Translation: CDH93081.1
FO081096 Genomic DNA Translation: CDH93082.1
FO081096 Genomic DNA Translation: CDH93083.1
RefSeqiNP_001023420.2, NM_001028249.3 [U4PR86-2]
NP_001023421.2, NM_001028250.2 [U4PR86-3]
NP_001294353.1, NM_001307424.1 [U4PR86-1]
NP_001294354.1, NM_001307425.1 [U4PR86-4]
NP_001294355.1, NM_001307426.1 [U4PR86-5]
NP_001294356.1, NM_001307427.1 [U4PR86-6]
NP_001294357.1, NM_001307428.1 [U4PR86-7]
NP_001294358.1, NM_001307429.1 [U4PR86-8]
UniGeneiCel.5632

Genome annotation databases

EnsemblMetazoaiW03G1.6c; W03G1.6c; WBGene00021012 [U4PR86-1]
GeneIDi176877
KEGGicel:CELE_W03G1.6
UCSCiW03G1.6a c. elegans

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMELK_CAEEL
AccessioniPrimary (citable) accession number: U4PR86
Secondary accession number(s): A7WK47
, A7WK48, U4PB44, U4PBH9, U4PED2, U4PLZ7, U4PR90
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 4, 2015
Last sequence update: December 11, 2013
Last modified: June 20, 2018
This is version 40 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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