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Protein

Lipoyl synthase

Gene

lipA

Organism
Ochrobactrum sp. EGD-AQ16
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N6-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (lipB)
  2. Lipoyl synthase (lipA)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi60Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi65Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi71Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi86Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi90Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi93Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotation
Ligand4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
ORF Names:O206_02065Imported
OrganismiOchrobactrum sp. EGD-AQ16Imported
Taxonomic identifieri1390361 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeOchrobactrum
Proteomesi
  • UP000016518 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliU1X6S6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini76 – 283Elp3InterPro annotationAdd BLAST208

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

OrthoDBiPOG091H069D.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR031691. LIAS_N.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
PfamiView protein in Pfam
PF16881. LIAS_N. 1 hit.
PF04055. Radical_SAM. 1 hit.
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SFLDiSFLDG01058. lipoyl_synthase_like. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
SMARTiView protein in SMART
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

U1X6S6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTVLDLVNQ GKRERHPEKA HRPDNVVLKK PDWIRVKAPV SRGYSETRDI
60 70 80 90 100
VRSNKLVTVC EEAGCPNIGE CWEKKHATFM IMGEICTRAC AFCNVSTGIP
110 120 130 140 150
TALDPNEPEN VAKAVKQMGL THVVITSVDR DDLADGGAQH FAEVIQAIRE
160 170 180 190 200
ATPATTIEIL TPDFLRKEGA LEIVVKARPD VFNHNLETVP SKYLKVRPGA
210 220 230 240 250
RYFHSIRLLQ RVKELDPTIF TKSGIMVGLG EERNEILQLM DDLRSADVDF
260 270 280 290 300
MTIGQYLQPT RKHHPVIRFV TPDEFKSFET IGRTKGFLLV ASSPLTRSSH
310 320
HAGDDFAKLR AAREAQIAAR A
Length:321
Mass (Da):35,884
Last modified:November 13, 2013 - v1
Checksum:iFDC41674CF295651
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AWEU01000001 Genomic DNA. Translation: ERI16544.1.
RefSeqiWP_006470529.1. NZ_AWEU01000001.1.

Genome annotation databases

EnsemblBacteriaiERI16544; ERI16544; O206_02065.
PATRICifig|1390361.3.peg.415.

Similar proteinsi

Entry informationi

Entry nameiU1X6S6_9RHIZ
AccessioniPrimary (citable) accession number: U1X6S6
Entry historyiIntegrated into UniProtKB/TrEMBL: November 13, 2013
Last sequence update: November 13, 2013
Last modified: October 25, 2017
This is version 35 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteomeImported