tilS

Functionⁱ

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.UniRule annotationSAAS annotation

Catalytic activityⁱ

(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H₂O.UniRule annotationSAAS annotation

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	25 – 30	ATPUniRule annotation		6

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-UniRule
ligase activity, forming carbon-nitrogen bonds Source: UniProtKB-UniRule

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

tRNA modification Source: UniProtKB-UniRule

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	LigaseUniRule annotationSAAS annotation
Biological process	tRNA processingUniRule annotationSAAS annotation
Ligand	ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: tRNA(Ile)-lysidine synthaseUniRule annotationSAAS annotation (EC:6.3.4.19UniRule annotationSAAS annotation) Alternative name(s): tRNA(Ile)-2-lysyl-cytidine synthaseUniRule annotation tRNA(Ile)-lysidine synthetaseUniRule annotation
Gene namesⁱ	Name:tilSUniRule annotation ORF Names:N399_00390Imported
Organismⁱ	Bacillus licheniformis CG-B52Imported
Taxonomic identifierⁱ	1368424 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Terrabacteria group › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus licheniformis
Proteomesⁱ	UP000017025 Componentⁱ: Unassembled WGS sequence

Subcellular locationⁱ

Cytoplasm
UniRule annotation
Automatic assertion according to rulesⁱ
- HAMAP-Rule:MF_01161
SAAS annotation
Automatic assertion according to rulesⁱ
- SAAS:SAAS00749588

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

CytoplasmUniRule annotationSAAS annotation

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	385 – 458	TilS_CInterPro annotationAdd BLAST		74

Domainⁱ

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.UniRule annotation

Sequence similaritiesⁱ

Belongs to the tRNA(Ile)-lysidine synthase family.UniRule annotationSAAS annotation

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd01992. PP-ATPase. 1 hit.
Gene3Dⁱ	3.40.50.620. 2 hits.
HAMAPⁱ	MF_01161. tRNA_Ile_lys_synt. 1 hit.
InterProⁱ	View protein in InterPro IPR012796. Lysidine-tRNA-synth_C. IPR014729. Rossmann-like_a/b/a_fold. IPR011063. TilS/TtcA_N. IPR012094. tRNA_Ile_lys_synt. IPR012795. tRNA_Ile_lys_synt_N. IPR015262. tRNA_Ile_lys_synt_subst-bd.
PANTHERⁱ	PTHR43033:SF1. PTHR43033:SF1. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01171. ATP_bind_3. 1 hit. PF09179. TilS. 1 hit. PF11734. TilS_C. 1 hit.
SMARTⁱ	View protein in SMART SM00977. TilS_C. 1 hit.
TIGRFAMsⁱ	TIGR02433. lysidine_TilS_C. 1 hit. TIGR02432. lysidine_TilS_N. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

T5HW33-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKSIEDFMKK YELSFDGVTL IAGVSGGPDS MALLHALHHT VPASATLIAA 
        60         70         80         90        100
HVDHMFRGEE SERDMRFVQD YCEAEGIQCE AVQIDVLAFA AENNLNKQAA 
       110        120        130        140        150
ARECRYAFFQ ELMKRHRAEY LVLGHHGDDQ VETMLMKMAK GTVGIGLAGI 
       160        170        180        190        200
QPKRRFDSGW LLRPFLRLSK DDLLAYCKEN RIPFRTDPSN SEDDYTRNRF 
       210        220        230        240        250
RHHVLPFLKK ESEDVHKRFQ SVSEFLTEDE LYLQALTKDK MNTVITSKSS 
       260        270        280        290        300
SGVEISIERL LALPMPLQRR GVHLILNYLY ENVPSAFSAH HIQVFLDWIS 
       310        320        330        340        350
QDGPSGSLDF PNGLKVAKSY HTCLFTFQRL QCENVSYHYE ISGAGEEELI 
       360        370        380        390        400
LPGGSSLHVS GPGSTRKLQG NDVFATSPKQ VQFPLYVRTR QNGDRVKLKG 
       410        420        430        440        450
MNGSKKVKDI FIDEKVPLSK RDSWPIVTDA VGNIIWIPGL KKTIFEELDV 
       460        470 
TNNDRIVLQY RQHEKCRGLA KNETGY

Length:476

Mass (Da):54,014

Last modified:November 13, 2013 - v1

Checksum:ⁱ6FFE55C19E785FBD

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AVEZ01000023 Genomic DNA. Translation: EQM28527.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_016886275.1. NZ_AVEZ01000023.1.

Genome annotation databases

EnsemblBacteriaⁱ	EQM28527; EQM28527; N399_00390.
PATRICⁱ	fig\|1368424.3.peg.1128.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
T5HW33	A0A1Y0YFH3	Bacillus licheniformis	476	UniRef100_A0A1Y0YFH3	Cluster: tRNA(Ile)-lysidine synthase	2

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
T5HW33	Q65PF4 A0A1Y0YFH3 R9TQU0 A0A0D7XNC4 A0A0D7XC87 A0A0D7XG25 A0A1Q8GRX4 A0A1R1RPY0 W7RNT0 UPI00025A9B48 +7	Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46) Bacillus licheniformis Bacillus paralicheniformis ATCC 9945a Bacillus amyloliquefaciens (Bacillus velezensis) Bacillus haynesii Bacillus licheniformis S 16 Bacillus Bacillus paralicheniformis Bacillus sp. SB47	476	UniRef90_Q65PF4	Cluster: tRNA(Ile)-lysidine synthase	18

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
T5HW33	Q65PF4 UPI0003A20DA3 R9TQU0 A0A0D7XNC4 A0A0D7XC87 A0A0D7XG25 UPI000502DB01 UPI00025A9B48 UPI00080DBD92 UPI000426F3C6 +24	Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46) Bacillus paralicheniformis Bacillus paralicheniformis ATCC 9945a Bacillus amyloliquefaciens (Bacillus velezensis) Bacillus Bacillus sp. SB47 Bacillus haynesii Bacillus licheniformis S 16 Bacillus licheniformis Bacillus glycinifermentans And more	476	UniRef50_Q65PF4	Cluster: tRNA(Ile)-lysidine synthase	35

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AVEZ01000023 Genomic DNA. Translation: EQM28527.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_016886275.1. NZ_AVEZ01000023.1.

3D structure databases

ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	EQM28527; EQM28527; N399_00390.
PATRICⁱ	fig\|1368424.3.peg.1128.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd01992. PP-ATPase. 1 hit.
Gene3Dⁱ	3.40.50.620. 2 hits.
HAMAPⁱ	MF_01161. tRNA_Ile_lys_synt. 1 hit.
InterProⁱ	View protein in InterPro IPR012796. Lysidine-tRNA-synth_C. IPR014729. Rossmann-like_a/b/a_fold. IPR011063. TilS/TtcA_N. IPR012094. tRNA_Ile_lys_synt. IPR012795. tRNA_Ile_lys_synt_N. IPR015262. tRNA_Ile_lys_synt_subst-bd.
PANTHERⁱ	PTHR43033:SF1. PTHR43033:SF1. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01171. ATP_bind_3. 1 hit. PF09179. TilS. 1 hit. PF11734. TilS_C. 1 hit.
SMARTⁱ	View protein in SMART SM00977. TilS_C. 1 hit.
TIGRFAMsⁱ	TIGR02433. lysidine_TilS_C. 1 hit. TIGR02432. lysidine_TilS_N. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	T5HW33_BACLI
Accessionⁱ	T5HW33Primary (citable) accession number: T5HW33
Entry historyⁱ	Integrated into UniProtKB/TrEMBL:	November 13, 2013
	Last sequence update:	November 13, 2013
	Last modified:	February 28, 2018
	This is version 27 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Unreviewed (UniProtKB/TrEMBL)

Miscellaneousⁱ

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termⁱ

Complete proteomeImported

UniProtKB - T5HW33 (T5HW33_BACLI)

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tRNA(Ile)-lysidine synthase

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Functionⁱ

Catalytic activityⁱ

Regions

GO - Molecular functionⁱ

GO - Biological processⁱ

Keywordsⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Family & Domainsⁱ

Domains and Repeats

Domainⁱ

Sequence similaritiesⁱ

Family and domain databases

Sequenceⁱ

Sequence databases

Genome annotation databases

Similar proteinsⁱ

Cross-referencesⁱ

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Family and domain databases

Entry informationⁱ

Miscellaneousⁱ

Caution

Keywords - Technical termⁱ

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - T5HW33 (T5HW33_BACLI)

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tRNA(Ile)-lysidine synthase

tilS

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Caution

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ