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Protein

Histidine biosynthesis bifunctional protein HisIE

Gene

hisI

Organism
Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

1-(5-phospho-beta-D-ribosyl)-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide.UniRule annotationSAAS annotation
1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-AMP + diphosphate.UniRule annotationSAAS annotation

Pathwayi: L-histidine biosynthesis

This protein is involved in step 2 and 3 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotationSAAS annotation
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (hisG)
  2. Histidine biosynthesis bifunctional protein HisIE (hisI)
  3. Histidine biosynthesis bifunctional protein HisIE (hisI), Phosphoribosyl-AMP cyclohydrolase (hisI)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
  5. Imidazole glycerol phosphate synthase subunit HisF (hisF), Imidazole glycerol phosphate synthase subunit HisH (hisH)
  6. Histidine biosynthesis bifunctional protein HisB (hisB)
  7. Histidinol-phosphate aminotransferase (hisC)
  8. Histidine biosynthesis bifunctional protein HisB (hisB)
  9. Histidinol dehydrogenase (hisD)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotationImported
Biological processAmino-acid biosynthesis, Histidine biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00031; UER00007.
UPA00031; UER00008.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine biosynthesis bifunctional protein HisIEUniRule annotation
Including the following 2 domains:
Phosphoribosyl-ATP pyrophosphataseUniRule annotation (EC:3.6.1.31UniRule annotation)
Short name:
PRA-PHUniRule annotation
Phosphoribosyl-AMP cyclohydrolaseUniRule annotation (EC:3.5.4.19UniRule annotation)
Short name:
PRA-CHUniRule annotation
Gene namesi
Name:hisIUniRule annotation
Synonyms:hisIEUniRule annotation
ORF Names:MADE_000001022230Imported
OrganismiAlteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)Imported
Taxonomic identifieri1774373 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeAlteromonas
Proteomesi
  • UP000001870 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliT2DLG1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 107PRA-CHInterPro annotationAdd BLAST74

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 116Phosphoribosyl-AMP cyclohydrolaseUniRule annotationAdd BLAST116
Regioni117 – 205Phosphoribosyl-ATP pyrophosphohydrolaseUniRule annotationAdd BLAST89

Sequence similaritiesi

In the C-terminal section; belongs to the PRA-PH family.UniRule annotationSAAS annotation
In the N-terminal section; belongs to the PRA-CH family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105K8F. Bacteria.
COG0139. LUCA.
COG0140. LUCA.
KOiK11755.

Family and domain databases

CDDicd11534. NTP-PPase_HisIE_like. 1 hit.
HAMAPiMF_01020. HisE. 1 hit.
MF_01019. HisIE. 1 hit.
InterProiView protein in InterPro
IPR023019. His_synth_HisIE.
IPR008179. HisE.
IPR021130. PRib-ATP_PPHydrolase-like.
IPR002496. PRib_AMP_CycHydrolase_dom.
PfamiView protein in Pfam
PF01502. PRA-CH. 1 hit.
PF01503. PRA-PH. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002610. PRA_CycHdrlase. 1 hit.
TIGRFAMsiTIGR03188. histidine_hisI. 1 hit.

Sequencei

Sequence statusi: Complete.

T2DLG1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIITNENSNK LAWDKMDNLL PAIVQDALSG KVLMQGYMDQ DALTKTFETG
60 70 80 90 100
KVTFFSRSKQ RLWTKGETSG NTLDLVSVAC DCDQDSLLVL ATPNGPTCHT
110 120 130 140 150
GAESCWFDGN TPAFTFLADL ERVLAARKDA DPKSSYTASL YNKGIKRIAQ
160 170 180 190 200
KVGEEGVETA LAATVHDKEE LKNEVADLLY HLTVLLQASD MSLNDALNVL

RERHK
Length:205
Mass (Da):22,586
Last modified:November 13, 2013 - v1
Checksum:iEADC1492BDDB63D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001103 Genomic DNA. Translation: AGV53800.1.
RefSeqiWP_012519070.1. NC_011138.3.

Genome annotation databases

EnsemblBacteriaiAGV53800; AGV53800; MADE_000001022230.
KEGGiamc:MADE_000001022230.

Similar proteinsi

Entry informationi

Entry nameiT2DLG1_ALTMD
AccessioniPrimary (citable) accession number: T2DLG1
Entry historyiIntegrated into UniProtKB/TrEMBL: November 13, 2013
Last sequence update: November 13, 2013
Last modified: August 30, 2017
This is version 35 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported, Multifunctional enzymeUniRule annotationSAAS annotation