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Protein

Regulatory protein E2

Gene

E2

Organism
Human papillomavirus type 18
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Plays a role in the initiation of viral DNA replication. A dimer of E2 interacts with a dimer of E1 in order to improve specificity of E1 DNA binding activity. Once the complex recognizes and binds DNA at specific sites, the E2 dimer is removed from DNA. E2 also regulates viral transcription through binding to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory regions of the viral genome. Activates or represses transcription depending on E2RE's position with regards to proximal promoter elements including the TATA-box. Repression occurs by sterically hindering the assembly of the transcription initiation complex.UniRule annotationSAAS annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivatorUniRule annotationSAAS annotation, DNA-bindingUniRule annotationSAAS annotation, RepressorUniRule annotationSAAS annotation
Biological processDNA replicationUniRule annotationSAAS annotation, Transcription, Transcription regulationUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein E2UniRule annotationSAAS annotation
Gene namesi
Name:E2UniRule annotationImported
OrganismiHuman papillomavirus type 18Imported
Taxonomic identifieri333761 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePapillomaviridaeAlphapapillomavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000112289 Componenti: Genome

Subcellular locationi

  • Host nucleus UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleusUniRule annotationSAAS annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki293Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)UniRule annotation

Post-translational modificationi

Phosphorylated.UniRule annotation
Sumoylation plays a regulatory role in E2 transcriptional activity.UniRule annotation

Keywords - PTMi

Isopeptide bondUniRule annotation, PhosphoproteinUniRule annotation, Ubl conjugationUniRule annotation

Expressioni

Keywords - Developmental stagei

Early proteinUniRule annotation

Interactioni

Subunit structurei

Binds DNA as homodimer. Interacts with protein E1; this interaction greatly increases E1 DNA-binding activity. Interacts with protein L1; this interaction enhances E2-dependent replication and transcription activation. Interacts with protein L2; this interaction inhibits E2 transcriptional activity but not DNA replication function E2. Interacts with protein E7; this interaction inhibits E7 oncogenic activity. Interacts with host TAF1; this interaction modulates E2-dependent transcriptional regulation. Interacts with host BRD4; this interaction mediates E2 transcriptional activation function. Additionally, the interaction with host BRD4 on mitotic chromosomes mediates tethering of the viral genome. Interacts with host TOPBP1; this interaction is required for optimal viral DNA replication.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 200PPV_E2_NInterPro annotationAdd BLAST195
Domaini288 – 360PPV_E2_CInterPro annotationAdd BLAST73

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni286 – 365DNA-binding domainUniRule annotationAdd BLAST80

Sequence similaritiesi

Belongs to the papillomaviridae E2 protein family.UniRule annotationSAAS annotation

Family and domain databases

HAMAPiMF_04001. PPV_E2. 1 hit.
InterProiView protein in InterPro
IPR035975. E2/EBNA1_C_sf.
IPR000427. Papillomavirus_E2_C.
IPR001866. Papillomavirus_E2_N.
IPR033668. Reg_prot_E2.
IPR036050. Regulatory_protein_E2_N.
PfamiView protein in Pfam
PF00511. PPV_E2_C. 1 hit.
PF00508. PPV_E2_N. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000672. E2_early_recog_prot_C. 1 hit.
SUPFAMiSSF51332. SSF51332. 1 hit.
SSF54957. SSF54957. 1 hit.

Sequencei

Sequence statusi: Complete.

T2A558-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTPKETLSE RLSVVQDKII DHYENDSKDI DSQIQYWQLI RWENAIFFAA
60 70 80 90 100
REHGIQTLNH QVVPAYNISK SKAHKAIELQ MALQGLAQSA YKTEDWTLQD
110 120 130 140 150
TCEELWNTEP THCFKKGGQT VQVYFDGNKD NCMNYVAWDS VYYMTDAGTW
160 170 180 190 200
DKTATCVSHR GLYYVKEGYN TFYIEFKSEC EKYGNTGTWE VHFGNNVIDC
210 220 230 240 250
NDSMCSTSDD TVSATQLVKQ LQHTPSPYSS TVSVGTAKTY GQTSAATRPG
260 270 280 290 300
HCGLAEKQHC GPVNPLLGAA TATGNNKRRK LCSGNTTPII HLKGDRNSLK
310 320 330 340 350
CLRYRLRKHS DHYRDISSTW HWTGAGNEKT GILTVTYHSE TQRTKFLNTV
360
AIPDSVQILV GYMTM
Length:365
Mass (Da):41,277
Last modified:November 13, 2013 - v1
Checksum:i1F72CDA78F813732
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KC470212 Genomic DNA. Translation: AGU90282.1.

Similar proteinsi

Entry informationi

Entry nameiT2A558_HPV18
AccessioniPrimary (citable) accession number: T2A558
Entry historyiIntegrated into UniProtKB/TrEMBL: November 13, 2013
Last sequence update: November 13, 2013
Last modified: November 22, 2017
This is version 18 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteomeImported