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Protein

Endolysin

Gene
N/A
Organism
Enterobacteria phage T7M
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Endolysin with amidase activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and breaking down the peptidoglycan layer.UniRule annotation
Plays an important role in the switch between viral transcription and genome replication. Once produced in sufficient amount, interacts with and inhibits the viral RNA polymerase that becomes unable to produce additional late transcripts. This lysozyme-polymerase complex in turn plays an active role in viral genome replication and packaging.UniRule annotation

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Zn2+ is required for amidase activity.UniRule annotation

Enzyme regulationi

Binding to the viral RNA polymerase inhibits amidase activity.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi18ZincUniRule annotation1
Sitei47Essential for amidase activity and zinc hydrate coordinationUniRule annotation1
Metal bindingi123ZincUniRule annotation1
Metal bindingi131ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntimicrobial, Bacteriolytic enzymeUniRule annotation, HydrolaseUniRule annotation
Biological processCytolysis, Host cell lysis by virusUniRule annotation, Viral release from host cell
LigandMetal-bindingUniRule annotation, ZincUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
EndolysinUniRule annotation (EC:3.5.1.28UniRule annotation)
Alternative name(s):
N-acetylmuramoyl-L-alanine amidaseUniRule annotation
OrganismiEnterobacteria phage T7MImported
Taxonomic identifieri1075774 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeAutographivirinaeT7virus
Proteomesi
  • UP000016454 Componenti: Genome

Subcellular locationi

  • Host cytoplasm UniRule annotation
  • Note: The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasmUniRule annotation

Expressioni

Keywords - Developmental stagei

Late proteinUniRule annotation

Interactioni

Subunit structurei

Interacts with the viral RNA polymerase.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliT1PTT1
SMRiT1PTT1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 151Ami_2InterPro annotationAdd BLAST151
Domaini1 – 124PGRPInterPro annotationAdd BLAST124

Sequence similaritiesi

Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.UniRule annotation

Family and domain databases

CDDicd06583 PGRP, 1 hit
Gene3Di3.40.80.101 hit
HAMAPiMF_04111 ENDOLYSIN_T7, 1 hit
InterProiView protein in InterPro
IPR036505 Amidase/PGRP_sf
IPR002502 Amidase_domain
IPR034689 Endolysin_T7_type
IPR015510 PGRP
IPR006619 PGRP_domain_met/bac
PANTHERiPTHR11022 PTHR11022, 1 hit
PfamiView protein in Pfam
PF01510 Amidase_2, 1 hit
SMARTiView protein in SMART
SM00644 Ami_2, 1 hit
SM00701 PGRP, 1 hit
SUPFAMiSSF55846 SSF55846, 1 hit

Sequencei

Sequence statusi: Complete.

T1PTT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKVQFKPRA TTEAIFVHCS ATKPSQNVGV REIRQWHKEQ GWLDVGYHFI
60 70 80 90 100
IKRDGTVEAG RDELAVGSHA KGYNHNSIGV CLVGGIDDKG KFDANFTPAQ
110 120 130 140 150
MQSLRSLLVT LLAKYEGSVL RAHHDVAPKA CPSFDLKRWW EKNELVTSDR

G
Length:151
Mass (Da):16,874
Last modified:November 13, 2013 - v1
Checksum:i2C0880FFC920DE9E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JX421753 Genomic DNA Translation: AFQ97047.1

Similar proteinsi

Entry informationi

Entry nameiT1PTT1_BPT3
AccessioniPrimary (citable) accession number: T1PTT1
Entry historyiIntegrated into UniProtKB/TrEMBL: November 13, 2013
Last sequence update: November 13, 2013
Last modified: February 28, 2018
This is version 21 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported