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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Avibacterium paragallinarum JF4211
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityUniRule annotation1
Active sitei2NucleophileUniRule annotation1
Metal bindingi304MagnesiumUniRule annotation1
Metal bindingi366MagnesiumUniRule annotation1
Metal bindingi367MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferaseUniRule annotationImported, Transferase
Biological processPurine biosynthesisUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Short name:
GPATaseUniRule annotation
Gene namesi
Name:purFUniRule annotation
ORF Names:AJF4211_001130Imported
OrganismiAvibacterium paragallinarum JF4211Imported
Taxonomic identifieri1351754 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeAvibacterium
Proteomesi
  • UP000015362 Componenti: Unassembled WGS sequence

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 235Glutamine amidotransferase type-2InterPro annotationAdd BLAST234

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferaseUniRule annotation

Family and domain databases

CDDicd00715. GPATase_N. 1 hit.
cd06223. PRTases_typeI. 1 hit.
Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF. 1 hit.
InterProiView protein in InterPro
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
IPR035584. PurF_N.
PfamiView protein in Pfam
PF00156. Pribosyltran. 1 hit.
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiView protein in PROSITE
PS51278. GATASE_TYPE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

S6EE47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIVGIIGQ SPVNQAIYDG LTVLQHRGQD AAGIVTIDDE NRFRLRKANG
60 70 80 90 100
LVSDVFQQVH MLRLQGNAGI GHVRYPTAGS SSVSEAQPFY VNSPYGLTLV
110 120 130 140 150
HNGNLTNSEQ LKKTLFERAR RHVNTNSDSE VLLNILAYHL DHIYTQHLSA
160 170 180 190 200
EDIFHAIKAT NNDIRGAYAC IAMIIGHGMV AFRDPNGIRP LVLGKREEKG
210 220 230 240 250
KTEYMFASES VALDVVGFEF VRDVQPGEAV YITFDGKLYA QQCADKPKLN
260 270 280 290 300
PCIFEYVYFA RPDSYIDGVS VYGARVHMGE RLGEKIAREW PDLDIDVVIP
310 320 330 340 350
VPETSNDIAL QIAHILNKPY RQGFVKNRYV GRTFIMPGQT QRASAVRRKL
360 370 380 390 400
NTISSEFKGK NVLLVDDSIV RGTTSQQIVD MARAAGAKKI YFASAAPEIR
410 420 430 440 450
YPNVYGIDMP TKHELIAYGR DVDEIAKLIG VDKLIFQDLD ALTQSVQQEN
460 470 480 490 500
PAIKGFDCSV FTGEYITGDI TAEYLDQIAD LRNDSAKKKR EKDATNLEMH

NES
Length:503
Mass (Da):56,023
Last modified:October 16, 2013 - v1
Checksum:iB805946B0FD20F07
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CBMK010000028 Genomic DNA. Translation: CDF99397.1.
RefSeqiWP_017806541.1. NZ_CBMK010000028.1.

Similar proteinsi

Entry informationi

Entry nameiS6EE47_AVIPA
AccessioniPrimary (citable) accession number: S6EE47
Entry historyiIntegrated into UniProtKB/TrEMBL: October 16, 2013
Last sequence update: October 16, 2013
Last modified: September 27, 2017
This is version 31 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteome, Reference proteomeImported