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Protein

Succinyl-diaminopimelate desuccinylase

Gene

dapE

Organism
Escherichia coli KTE38
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls.SAAS annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

N-succinyl-LL-2,6-diaminoheptanedioate + H2O = succinate + LL-2,6-diaminoheptanedioate.UniRule annotationSAAS annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 3 of the subpathway that synthesizes LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route).UniRule annotationSAAS annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD)
  2. Acetylornithine/succinyldiaminopimelate aminotransferase (argD)
  3. Succinyl-diaminopimelate desuccinylase (dapE)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route), the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi66Cobalt or zinc 1UniRule annotation1
Active sitei68UniRule annotation1
Metal bindingi99Cobalt or zinc 1UniRule annotation1
Metal bindingi99Cobalt or zinc 2UniRule annotation1
Active sitei133Proton acceptorUniRule annotation1
Metal bindingi134Cobalt or zinc 2UniRule annotation1
Metal bindingi162Cobalt or zinc 1UniRule annotation1
Metal bindingi348Cobalt or zinc 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotation
Biological processAmino-acid biosynthesis, Diaminopimelate biosynthesisUniRule annotationSAAS annotation, Lysine biosynthesisUniRule annotationSAAS annotation
LigandCobaltUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, ZincUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00034; UER00021

Names & Taxonomyi

Protein namesi
Recommended name:
Succinyl-diaminopimelate desuccinylaseUniRule annotationSAAS annotation (EC:3.5.1.18UniRule annotation)
Short name:
SDAP desuccinylaseUniRule annotation
Alternative name(s):
N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolaseUniRule annotation
Gene namesi
Name:dapEUniRule annotation
ORF Names:WG7_03064Imported
OrganismiEscherichia coli KTE38Imported
Taxonomic identifieri1169355 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000014196 Componenti: Unassembled WGS sequence

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliS0Z5J9
SMRiS0Z5J9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini175 – 282M20_dimerInterPro annotationAdd BLAST108

Sequence similaritiesi

Belongs to the peptidase M20A family. DapE subfamily.UniRule annotationSAAS annotation

Phylogenomic databases

OMAiLNSHHDT

Family and domain databases

CDDicd03891 M20_DapE_proteobac, 1 hit
HAMAPiMF_01690 DapE, 1 hit
InterProiView protein in InterPro
IPR001261 ArgE/DapE_CS
IPR036264 Bact_exopeptidase_dim_dom
IPR005941 DapE_proteobac
IPR002933 Peptidase_M20
IPR011650 Peptidase_M20_dimer
PfamiView protein in Pfam
PF07687 M20_dimer, 1 hit
PF01546 Peptidase_M20, 1 hit
SUPFAMiSSF55031 SSF55031, 1 hit
TIGRFAMsiTIGR01246 dapE_proteo, 1 hit
PROSITEiView protein in PROSITE
PS00758 ARGE_DAPE_CPG2_1, 1 hit
PS00759 ARGE_DAPE_CPG2_2, 1 hit

Sequencei

Sequence statusi: Complete.

S0Z5J9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCPVIELTQ QLIRRPSLSP DDAGCQALLI ERLQAIGFTV ERMDFADTQN
60 70 80 90 100
FWAWRGQGET LAFAGHTDVV PPGDADRWIN PPFEPTIRDG MLFGRGAADM
110 120 130 140 150
KGSLAAMVVA AERFVAQHPN HTGRLAFLIT SDEEASAHNG TVKVVEALMA
160 170 180 190 200
RNERLDYCLV GEPSSIEVVG DVVKNGRRGS LTCNLTIHGV QGHVAYPHLA
210 220 230 240 250
DNPVHRAAPF LNELVAIEWD QGNEFFPATS MQIANIQAGT GSNNVIPGEL
260 270 280 290 300
FVQFNFRFST ELTDEMIKAQ VLALLEKHQL RYTVDWWLSG QPFLTARGKL
310 320 330 340 350
VDAVVNAVEH YNEIKPQLLT TGGTSDGRFI ARMGAQVVEL GPVNATIHKI
360 370
NECVNAADLQ LLARMYQRIM EQLVA
Length:375
Mass (Da):41,269
Last modified:September 18, 2013 - v1
Checksum:i181F43D05E88C20E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ASUD01000067 Genomic DNA Translation: EOV05450.1
RefSeqiWP_001277801.1, NZ_KE136817.1

Genome annotation databases

EnsemblBacteriaiEOV05450; EOV05450; WG7_03064
PATRICifig|1169355.3.peg.3150

Similar proteinsi

Entry informationi

Entry nameiS0Z5J9_ECOLX
AccessioniPrimary (citable) accession number: S0Z5J9
Entry historyiIntegrated into UniProtKB/TrEMBL: September 18, 2013
Last sequence update: September 18, 2013
Last modified: March 28, 2018
This is version 33 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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