Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Apicidin F cluster transcription factor apf2

Gene

apf2

Organism
Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) (Bakanae and foot rot disease fungus) (Fusarium fujikuroi)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that regulates the expression of the gene cluster that mediates the biosynthesis of apicidin F (PubMed:23825955, PubMed:25058475). Binds to the eight-base-pair motif 5'-TGACGTGA-3' called the 'Api-box' that is found in all promoters of the apicidin F cluster except in the promoter region of apf2 itself (PubMed:25058475).2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Apicidin F cluster transcription factor apf21 Publication
Alternative name(s):
Apicidin F synthesis protein 21 Publication
Gene namesi
Name:apf21 Publication
ORF Names:FFUJ_00012
OrganismiGibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) (Bakanae and foot rot disease fungus) (Fusarium fujikuroi)
Taxonomic identifieri1279085 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusariumFusarium fujikuroi species complex
Proteomesi
  • UP000016800 Componenti: Chromosome 1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Biotechnological usei

Apicidin F, like the other known apicidins, is a cyclic tetrapeptides with anti-malarial properties via histone deacetylase inhibitory activity (PubMed:24195442).1 Publication

Disruption phenotypei

Leads to the loss of apicidin F production (PubMed:25058475).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004371681 – 389Apicidin F cluster transcription factor apf2Add BLAST389

Structurei

3D structure databases

SMRiS0DPL8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati241 – 270ANK 1Sequence analysisAdd BLAST30
Repeati274 – 303ANK 2Sequence analysisAdd BLAST30
Repeati307 – 336ANK 3Sequence analysisAdd BLAST30
Repeati357 – 386ANK 4Sequence analysisAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni12 – 38Basic DNA-binding region1 PublicationAdd BLAST27

Domaini

Contains a basic DNA-binding region at the N-terminus which is usually found in bZIP transcription factors, but does not contain the characteristic leucine zipper domain (PubMed:25058475). Instead, four C-terminal ankyrin repeats were identified that were shown to confer protein-protein interaction of regulatory proteins (PubMed:25058475).1 Publication

Sequence similaritiesi

Belongs to the bZIP family. Highly divergent.Curated

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

OrthoDBiEOG092C56RE.

Family and domain databases

CDDicd00204. ANK. 1 hit.
Gene3Di1.25.40.20. 1 hit.
InterProiView protein in InterPro
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR036770. Ankyrin_rpt-contain_sf.
PfamiView protein in Pfam
PF13606. Ank_3. 1 hit.
SMARTiView protein in SMART
SM00248. ANK. 4 hits.
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiView protein in PROSITE
PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.

Sequencei

Sequence statusi: Complete.

S0DPL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPPSQDWST ITDANERRKA QNRVAQRNYR SRQKLRVELA EAILYDMPSW
60 70 80 90 100
RTAVGLKRKR WLETPQSVED HDTNPDSATP KPSLSSQVIQ YHEEGQLVQN
110 120 130 140 150
PTDFSMSADA DVTADVSSGG ESSDLSLGVF TPGDDWMNAE IDWTAAMTLA
160 170 180 190 200
GDTSASEPDL RHRAVGPGND IHAQPPVSIP VQAASGQHRT LTAPTPAESM
210 220 230 240 250
SLRTEPPETA VITVTSNREV PNLGDSNEGS RSISTTKQPS EFKTPVLTAI
260 270 280 290 300
AKGKLDIARL LISSGANIDT RDMHGRTNLH YAVSRSDAKT VRSLLELGAD
310 320 330 340 350
LLMSDASGVT ALHMAVGADD QDMVKLLLGW CEQQDRTASS TDHKTTLKQC
360 370 380
INSRDGQNMT PLHLCVVMER MDMLKILLDY GADVNIGCD
Length:389
Mass (Da):42,375
Last modified:September 18, 2013 - v1
Checksum:iC1A6BA2C2EE4CD1B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HF679023 Genomic DNA. Translation: CCT63352.1.

Genome annotation databases

EnsemblFungiiCCT63352; CCT63352; FFUJ_00012.

Similar proteinsi

Entry informationi

Entry nameiAPF2_GIBF5
AccessioniPrimary (citable) accession number: S0DPL8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 7, 2016
Last sequence update: September 18, 2013
Last modified: November 22, 2017
This is version 22 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families