Reviewed, UniProtKB/Swiss-Prot Q8W4J9 (RPP8_ARATH)

Names and origin

Protein names

Recommended name:
    Disease resistance protein RPP8
Alternative name(s):
    Resistance to Peronospora parasitica protein 8

Gene names

Name:	RPP8
Synonyms:	HRT
Ordered Locus Names:	At5g43470
ORF Names:	MWF20.19

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	908 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Disease resistance protein. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth. The interaction with TIP (TCV-interacting protein) may be essential for the recognition of the avirulence proteins, and the triggering of the defense response.
Subunit structure	Interacts with the NAC protein TIP.
Domain	The LRR repeats probably act as specificity determinant of pathogen recognition.
Polymorphism	The strong polymorphisms present in cv. Di-17 and cv. Columbia are probably due to an unequal crossing-over between the highly related RPP8 and RPH8A genes present in cv. Landsberg erecta. Such variations probably modify the specificity of pathogen recognition.
Miscellaneous	In cv. Columbia and cv. Landsberg erecta, RPP8 specifically recognizes the Emco5 avirulence protein from Peronospora parasitica, while it is not the case in cv. Di-17, where it confers resistance to Turnip Crinkle Virus uppon recognition of the viral capsid protein.
Sequence similarities	Belongs to the disease resistance NB-LRR family. RPP8/HRT subfamily. Contains 2 LRR (leucine-rich) repeats. Contains 1 NB-ARC domain.

Ontologies

Keywords
Biological process	Hypersensitive response Plant defense
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Leucine-rich repeat Repeat
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	plant-type hypersensitive response Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8W4J9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8W4J9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     294-308: WKMLLTSRNEGVGIH → ELLWYIHEALFLLNS
     309-908: Missing.

Notes: Has been shown to exist only in cv. Columbia so far.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 908	908	Disease resistance protein RPP8		PRO_0000212719
Regions
	Domain	146 – 459	314	NB-ARC
	Repeat	600 – 623	24	LRR 1
	Repeat	842 – 867	26	LRR 2
	Nucleotide binding	192 – 199	8	ATP Potential
	Coiled coil	15 – 57	43	Potential
Natural variations
	Alternative sequence	294 – 308	15	WKMLL…GVGIH → ELLWYIHEALFLLNS in isoform 2.		VSP_007171
	Alternative sequence	309 – 908	600	Missing in isoform 2.		VSP_007172
	Natural variant	27 – 31	5	IDGQL → VDEQI in strain: cv. Landsberg erecta.
	Natural variant	29	1	G → E in strain: cv. Di-17.
	Natural variant	85 – 87	3	SGK → RGE in strain: cv. Di-17 and cv. Landsberg erecta.
	Natural variant	88 – 91	4	GKGV → EKGI in strain: cv. Landsberg erecta.
	Natural variant	100	1	C → R in strain: cv. Landsberg erecta.
	Natural variant	121	1	E → D in strain: cv. Landsberg erecta.
	Natural variant	129	1	F → L in strain: cv. Di-17.
	Natural variant	133	1	Q → QQ in strain: cv. Di-17.
	Natural variant	138	1	G → V in strain: cv. Landsberg erecta.
	Natural variant	173	1	K → T in strain: cv. Di-17.
	Natural variant	177	1	G → C in strain: cv. Di-17.
	Natural variant	253	1	Y → S in strain: cv. Di-17.
	Natural variant	257	1	R → P in strain: cv. Di-17.
	Natural variant	265	1	A → T in strain: cv. Di-17.
	Natural variant	267	1	R → K in strain: cv. Landsberg erecta.
	Natural variant	270	1	V → L in strain: cv. Di-17.
	Natural variant	283	1	V → R in strain: cv. Di-17.
	Natural variant	369	1	A → V in strain: cv. Di-17 and cv. Landsberg erecta.
	Natural variant	387	1	F → S in strain: cv. Di-17 and cv. Landsberg erecta.
	Natural variant	399	1	W → C in strain: cv. Di-17 and cv. Landsberg erecta.
	Natural variant	426	1	C → R in strain: cv. Di-17 and cv. Landsberg erecta.
	Natural variant	429	1	N → F in strain: cv. Di-17 and cv. Landsberg erecta.
	Natural variant	436 – 446	11	DSEISTYSLFY → YSKISAYDLFN in strain: cv. Landsberg erecta.
	Natural variant	438 – 443	6	EISTYS → KITTQE in strain: cv. Di-17.
	Natural variant	450	1	A → V in strain: cv. Landsberg erecta.
	Natural variant	460	1	E → Q in strain: cv. Landsberg erecta.
	Natural variant	480 – 486	7	DNYLSWQ → NKYLRVH in strain: cv. Di-17.
	Natural variant	480 – 481	2	DN → NR in strain: cv. Landsberg erecta.
	Natural variant	485 – 486	2	WQ → SH in strain: cv. Landsberg erecta.
	Natural variant	489	1	Y → N in strain: cv. Landsberg erecta.
	Natural variant	514	1	I → K in strain: cv. Di-17 and cv. Landsberg erecta.
	Natural variant	518	1	C → S in strain: cv. Di-17 and cv. Landsberg erecta.
	Natural variant	519	1	T → I in strain: cv. Di-17.
	Natural variant	528	1	S → R in strain: cv. Di-17.
	Natural variant	537	1	S → R in strain: cv. Di-17.
	Natural variant	542	1	H → Q in strain: cv. Di-17.
	Natural variant	543	1	I → L in strain: cv. Landsberg erecta.
	Natural variant	547 – 550	4	KNKT → RNNA in strain: cv. Di-17.
	Natural variant	549	1	K → N in strain: cv. Landsberg erecta.
	Natural variant	558 – 565	8	PRFEEDYW → WDEDFG in strain: cv. Landsberg erecta.
	Natural variant	558 – 560	3	PRF → SRFK in strain: cv. Di-17.
	Natural variant	564	1	Y → F in strain: cv. Di-17.
	Natural variant	584	1	S → Y in strain: cv. Landsberg erecta.
	Natural variant	595	1	C → S in strain: cv. Di-17 and cv. Landsberg erecta.
	Natural variant	609 – 612	4	YEAK → FLAG in strain: cv. Landsberg erecta.
	Natural variant	630 – 635	6	NLRVDT → DLSVHE in strain: cv. Di-17.
	Natural variant	632 – 636	5	RVDTE → SVNNK in strain: cv. Landsberg erecta.
	Natural variant	650	1	Q → E in strain: cv. Di-17.
	Natural variant	653 – 654	2	YL → HI in strain: cv. Di-17.
	Natural variant	659	1	Missing in strain: cv. Di-17.
	Natural variant	660	1	M → K in strain: cv. Landsberg erecta.
	Natural variant	676 – 678	3	YLY → FLF in strain: cv. Landsberg erecta.
	Natural variant	678 – 679	2	YG → FR in strain: cv. Di-17.
	Natural variant	692	1	R → H in strain: cv. Landsberg erecta.
	Natural variant	697	1	R → Q in strain: cv. Di-17.
	Natural variant	700	1	A → G in strain: cv. Di-17.
	Natural variant	724	1	T → S in strain: cv. Di-17.
	Natural variant	726 – 727	2	NF → YV in strain: cv. Landsberg erecta.
	Natural variant	730 – 731	2	SL → TP in strain: cv. Di-17.
	Natural variant	731 – 734	4	LETY → PEIF in strain: cv. Landsberg erecta.
	Natural variant	752	1	Q → E in strain: cv. Di-17 and cv. Landsberg erecta.
	Natural variant	768	1	F → L in strain: cv. Landsberg erecta.
	Natural variant	773 – 781	9	VHLFLIYCG → AQIYICNCR in strain: cv. Landsberg erecta.
	Natural variant	773 – 781	9	VHLFLIYCG → THIHLLFCR in strain: cv. Di-17.
	Natural variant	800 – 807	8	RLARKAFL → QLTDEAFV in strain: cv. Di-17.
	Natural variant	800 – 803	4	RLAR → KLTF in strain: cv. Landsberg erecta.
	Natural variant	807 – 809	3	LGS → AGR in strain: cv. Landsberg erecta.
	Natural variant	819	1	P → T in strain: cv. Landsberg erecta.
	Natural variant	823 – 824	2	VI → AL in strain: cv. Di-17 and cv. Landsberg erecta.
	Natural variant	825	1	E → D in strain: cv. Di-17.
	Natural variant	828 – 829	2	KE → EQ in strain: cv. Landsberg erecta.
	Natural variant	851	1	D → H in strain: cv. Di-17 and cv. Landsberg erecta.
	Natural variant	854	1	K → E in strain: cv. Di-17 and cv. Landsberg erecta.
Experimental info
	Mutagenesis	418	1	D → N in rpp8-1; loss of function
	Mutagenesis	553	1	R → K in rpp8-2; loss of function
	Mutagenesis	830	1	S → L in rpp8-1; loss of function
	Sequence conflict	83	1	K → R in AAL32592. Ref.5
	Sequence conflict	686	1	S → G in AAL32592. Ref.5
	Sequence conflict	835	1	W → R in AAL32592. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 11, 2003. Version 2. Checksum: 1BFA35BB6B0CB5CD	FASTA	908	104,682
	10 20 30 40 50 60 MAEAFVSFGL EKLWDLLSRE SERLQGIDGQ LDGLKRQLRS LQSLLKDADA KKHGSDRVRN 70 80 90 100 110 120 FLEDVKDLVF DAEDIIESYV LNKLSGKGKG VKKHVRRLAC FLTDRHKVAS DIEGITKRIS 130 140 150 160 170 180 EVIGEMQSFG IQQIIDGGRS LSLQERQRVQ REIRQTYPDS SESDLVGVEQ SVKELVGHLV 190 200 210 220 230 240 ENDVHQVVSI AGMGGIGKTT LARQVFHHDL VRRHFDGFAW VCVSQQFTQK HVWQRILQEL 250 260 270 280 290 300 QPHDGDILQM DEYALQRKLF QLLEAGRYLV VLDDVWKKED WDVIKAVFPR KRGWKMLLTS 310 320 330 340 350 360 RNEGVGIHAD PTCLTFRASI LNPEESWKLC ERIVFPRRDE TEVRLDEEME AMGKEMVTHC 370 380 390 400 410 420 GGLPLAVKAL GGLLANKHTV PEWKRVFDNI GSQIVGGSWL DDNSLNSVYR ILSLSYEDLP 430 440 450 460 470 480 THLKHCFLNL AHFPEDSEIS TYSLFYYWAA EGIYDGSTIE DSGEYYLEEL VRRNLVIADD 490 500 510 520 530 540 NYLSWQSKYC QMHDMMREVC LSKAKEENFL QIIIDPTCTS TINAQSPSRS RRLSIHSGKA 550 560 570 580 590 600 FHILGHKNKT KVRSLIVPRF EEDYWIRSAS VFHNLTLLRV LDLSWVKFEG GKLPCSIGGL 610 620 630 640 650 660 IHLRYLSLYE AKVSHLPSTM RNLKLLLYLN LRVDTEEPIH VPNVLKEMIQ LRYLSLPLKM 670 680 690 700 710 720 DDKTKLELGD LVNLEYLYGF STQHSSVTDL LRMTKLRYLA VSLSERCNFE TLSSSLRELR 730 740 750 760 770 780 NLETLNFLFS LETYMVDYMG EFVLDHFIHL KQLGLAVRMS KIPDQHQFPP HLVHLFLIYC 790 800 810 820 830 840 GMEEDPMPIL EKLLHLKSVR LARKAFLGSR MVCSKGGFPQ LCVIEISKES ELEEWIVEEG 850 860 870 880 890 900 SMPCLRTLTI DDCKKLKELP DGLKYITSLK ELKIEGMKRE WKEKLVPGGE DYYKVQHIPD VQFINCDQ
	Isoform 2 [UniParc]. Checksum: F6F99BB4D03F6513		308	35,456
	10 20 30 40 50 60 MAEAFVSFGL EKLWDLLSRE SERLQGIDGQ LDGLKRQLRS LQSLLKDADA KKHGSDRVRN 70 80 90 100 110 120 FLEDVKDLVF DAEDIIESYV LNKLSGKGKG VKKHVRRLAC FLTDRHKVAS DIEGITKRIS 130 140 150 160 170 180 EVIGEMQSFG IQQIIDGGRS LSLQERQRVQ REIRQTYPDS SESDLVGVEQ SVKELVGHLV 190 200 210 220 230 240 ENDVHQVVSI AGMGGIGKTT LARQVFHHDL VRRHFDGFAW VCVSQQFTQK HVWQRILQEL 250 260 270 280 290 300 QPHDGDILQM DEYALQRKLF QLLEAGRYLV VLDDVWKKED WDVIKAVFPR KRGELLWYIH EALFLLNS

References

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[1]	"Intragenic recombination and diversifying selection contribute to the evolution of downy mildew resistance at the RPP8 locus of Arabidopsis." McDowell J.M., Dhandaydham M., Long T.A., Aarts M.G.M., Goff S., Holub E.B., Dangl J.L. Plant Cell 10:1861-1874(1998) [PubMed: 9811794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTANTS RPP8-1; RPP8-2 AND RPP8-3, VARIANTS. Strain: cv. Columbia and cv. Landsberg erecta.
[2]	"Members of the Arabidopsis HRT/RPP8 family of resistance genes confer resistance to both viral and oomycete pathogens." Cooley M.B., Pathirana S., Wu H.-J., Kachroo P., Klessig D.F. Plant Cell 12:663-676(2000) [PubMed: 10810142] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS. Strain: cv. Di-17.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Functional annotation of a full-l