ID   R4V5I5_9GAMM            Unreviewed;       880 AA.
AC   R4V5I5;
DT   24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT   24-JUL-2013, sequence version 1.
DT   24-JAN-2024, entry version 50.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE            Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE            Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE            EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN   Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595};
GN   ORFNames=SPISAL_01030 {ECO:0000313|EMBL:AGM40305.1};
OS   Spiribacter salinus M19-40.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Chromatiales;
OC   Ectothiorhodospiraceae; Spiribacter.
OX   NCBI_TaxID=1260251 {ECO:0000313|EMBL:AGM40305.1, ECO:0000313|Proteomes:UP000017881};
RN   [1] {ECO:0000313|EMBL:AGM40305.1, ECO:0000313|Proteomes:UP000017881}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=M19-40 {ECO:0000313|EMBL:AGM40305.1};
RX   PubMed=23409269;
RA   Leon M.J., Ghai R., Fernandez A.B., Sanchez-Porro C., Rodriguez-Valera F.,
RA   Ventosa A.;
RT   "Draft Genome of Spiribacter salinus M19-40, an Abundant
RT   Gammaproteobacterium in Aquatic Hypersaline Environments.";
RL   Genome Announc. 1:E00179-12(2013).
CC   -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC       for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC       ECO:0000256|HAMAP-Rule:MF_00595}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC         Rule:MF_00595};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00595};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC       {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
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DR   EMBL; CP005963; AGM40305.1; -; Genomic_DNA.
DR   RefSeq; WP_016352612.1; NC_021291.1.
DR   AlphaFoldDB; R4V5I5; -.
DR   KEGG; ssal:SPISAL_01030; -.
DR   PATRIC; fig|1260251.3.peg.206; -.
DR   eggNOG; COG2352; Bacteria.
DR   HOGENOM; CLU_006557_2_0_6; -.
DR   OrthoDB; 9768133at2; -.
DR   Proteomes; UP000017881; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW   Rule:MF_00595};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW   Pyruvate {ECO:0000313|EMBL:AGM40305.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000017881}.
FT   ACT_SITE        144
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT                   ECO:0000256|PROSITE-ProRule:PRU10111"
FT   ACT_SITE        549
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00595"
SQ   SEQUENCE   880 AA;  99333 MW;  C86BC767448FBE05 CRC64;
     MSENRSDAPL REDIRQLGGL LGSTIHEQAG ETVYETIEEV RGLAKDARAG DGAAAEALEK
     RLGELPDEMI VPVVRAFSQF LNLANIAEQH HRVRRSRAWV RDPDTGPLRG SLEEFFKRML
     PDTEPDDLYN TIAHLDIELV LTAHPTEVMR RSMLQKYNHI AGLLDEGDRV DPTPEEIEEV
     HQALKREITA AWETDEIRRR RPAPQDEARW GMAVIEQTLW DVVPHYLRRL DRMLQRQTGK
     SLPLNAAPIH FGSWMGGDRD GNPRVTSAVT REVCLLNRWK AVELYEREVG ELVEELSLQC
     ASDELRDWVG DAWEPYRTAL KAVRDRLLAT RHWIEARLAG RRPAEGLIYW RADELREPLM
     VCYRSLHESG AAVVAEGRLK DLLRRLSVFG LTLMRVDIRQ ESTRHTEVLD ALTQALGIGS
     YEAWSEEERQ RFLIREIDSR RPLIPRDFEP SDEVAEVLET LRVIAEMGAD SLGAYVISMA
     SKPSDILAVK LLQKEVGVVS PLRVVPLFET LTDLQGAQTC LEQLLAIPWY RQHIDGFQEV
     MIGYSDSAKD ASHLTAAWAL YQTQEQLVAT AREQGIRLIL FHGRGGSIGR GGGPTHAAIL
     SQPPGSVDGR LRVTEQGEVI QAKFGLRGIA ERNLELYTTA VAEATLAPPQ PPEANWRALM
     DQLAETSCEV YRTLVRETPE FIDYFRHATP EHEISHLAIG SRPARRKPTV GVESLRAIPW
     IFAWTQTRLL LPAWLGIGEA LRVALENGHE AELQRMYQHW PFFRAFVDMI EMVLAKSDPA
     VAAWYDNRLV PEALRPLGAG LRDRHAETRQ TVLTVSGHDN PLDEAPVVQR SVGVRNPYVD
     PLNLIQVELL DRVRHGHEED LREALMICIN GIAAGMRNTG
//