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Protein

Bifunctional protein FolD

Gene

folD

Organism
Enterococcus asini ATCC 700915
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.UniRule annotationSAAS annotation

Catalytic activityi

5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.UniRule annotationSAAS annotation
5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.UniRule annotationSAAS annotation

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei190NADPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi165 – 167NADPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotation, Multifunctional enzymeUniRule annotationSAAS annotation, OxidoreductaseUniRule annotationSAAS annotation
Biological processAmino-acid biosynthesis, Histidine biosynthesisUniRule annotationSAAS annotation, Methionine biosynthesisUniRule annotationSAAS annotation, One-carbon metabolismUniRule annotationSAAS annotation, Purine biosynthesisUniRule annotationSAAS annotation
LigandNADPUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein FolDUniRule annotation
Including the following 2 domains:
Methenyltetrahydrofolate cyclohydrolaseUniRule annotation (EC:3.5.4.9UniRule annotation)
Methylenetetrahydrofolate dehydrogenaseUniRule annotation (EC:1.5.1.5UniRule annotation)
Gene namesi
Name:folDUniRule annotation
ORF Names:UAS_01494Imported
OrganismiEnterococcus asini ATCC 700915Imported
Taxonomic identifieri1158606 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
Proteomesi
  • UP000013777 Componenti: Unassembled WGS sequence

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 120THF_DHG_CYHInterPro annotationAdd BLAST116
Domaini123 – 280THF_DHG_CYH_CInterPro annotationAdd BLAST158

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili9 – 29Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.UniRule annotationSAAS annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

OrthoDBiPOG091H0041.

Family and domain databases

HAMAPiMF_01576. THF_DHG_CYH. 1 hit.
InterProiView protein in InterPro
IPR036291. NAD(P)-bd_dom_sf.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
PANTHERiPTHR10025. PTHR10025. 1 hit.
PfamiView protein in Pfam
PF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiView protein in PROSITE
PS00766. THF_DHG_CYH_1. 1 hit.
PS00767. THF_DHG_CYH_2. 1 hit.

Sequencei

Sequence statusi: Complete.

R2S2F9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAKLIDGKQ LANLMQEQMK EEVAQLKERG ITPGLVVLLV GEDTASQVYV
60 70 80 90 100
RNKDRVAAKI GIHSKVERYP DTITEAELLA EIQRYNEDPA FHGILVQLPL
110 120 130 140 150
PKQIDENKVL LAIDPKKDVD GFHPLNLGAL FVGKPTKIPC TPYGIMKMFE
160 170 180 190 200
YYEIDLTGKN AVVIGRSNIV GKPMAHLLLG QNATVTVTHS KTKNLPEICR
210 220 230 240 250
QADILVVAIG QGNFVTKEFV KEGAVVIDVG MNRDENGKLI GDVKFDEVSP
260 270 280
LASYITPVPG GVGPMTITML MYQTISSATQ E
Length:281
Mass (Da):30,780
Last modified:June 26, 2013 - v1
Checksum:i5BD802047BBF5604
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJAP01000012 Genomic DNA. Translation: EOH87031.1.

Genome annotation databases

EnsemblBacteriaiEOH87031; EOH87031; UAS_01494.
PATRICifig|1158606.3.peg.1452.

Similar proteinsi

Entry informationi

Entry nameiR2S2F9_9ENTE
AccessioniPrimary (citable) accession number: R2S2F9
Entry historyiIntegrated into UniProtKB/TrEMBL: June 26, 2013
Last sequence update: June 26, 2013
Last modified: February 28, 2018
This is version 36 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteome, Reference proteomeImported