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Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Enterococcus faecium EnGen0191
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotationSAAS annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 magnesium or manganese ions per subunit.UniRule annotation
  • Mn2+SAAS annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi295Magnesium or manganese 1UniRule annotation1
Metal bindingi308Magnesium or manganese 1UniRule annotation1
Metal bindingi308Magnesium or manganese 2UniRule annotation1
Metal bindingi310Magnesium or manganese 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationSAAS annotationImported
Biological processCell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation
LigandATP-bindingPROSITE-ProRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotationSAAS annotation (EC:6.3.2.4UniRule annotationSAAS annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlUniRule annotation
ORF Names:SSI_00190Imported
OrganismiEnterococcus faecium EnGen0191Imported
Taxonomic identifieri1157486 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
Proteomesi
  • UP000013828 Componenti: Unassembled WGS sequence

Subcellular locationi

R2P0Y2:
  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliR2P0Y2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini136 – 341ATP-graspInterPro annotationAdd BLAST206

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotationSAAS annotation

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
HAMAPiMF_00047. Dala_Dala_lig. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
PfamiView protein in Pfam
PF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
PIRSFiPIRSF039102. Ddl/VanB. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

R2P0Y2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKITLLYGGR SAEHEVSILS AFSVLNAIYY NYYQVQLIFI TKEGQWVKGP
60 70 80 90 100
LLTEKPASKD VLHLSWDPSG QTEEGFTGKV INPGEIKEEG AIVFPVLHGP
110 120 130 140 150
NGEDGTIQGF LETLNMPYVG AGVLTSACAM DKIMTKYILQ AAGVPQVPYV
160 170 180 190 200
PVLKNQWKEN PKKVFDQCEG SLLYPMFVKP ANMGSSVGIT KAENREELQN
210 220 230 240 250
ALATAYQYDS RAIVEQGIEA REIEVAVLGN EDVRTTLPGE VVKDVAFYDY
260 270 280 290 300
EAKYINNKIE MQIPAEVPEE VYQKAQEYAK LAYTMLGGSG LSRCDFFLTN
310 320 330 340 350
KNELFLNELN SMPGFTEFSM YPLLWENMGL KYGDLIEELI QLGMNRYHQR

QSFFEKNE
Length:358
Mass (Da):40,261
Last modified:June 26, 2013 - v1
Checksum:i2DE60FC943EF7CE6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AITZ01000001 Genomic DNA. Translation: EOH49906.1.
RefSeqiWP_002307390.1. NZ_KB949474.1.

Genome annotation databases

EnsemblBacteriaiEOH49906; EOH49906; SSI_00190.
PATRICifig|1157486.3.peg.193.

Similar proteinsi

Entry informationi

Entry nameiR2P0Y2_ENTFC
AccessioniPrimary (citable) accession number: R2P0Y2
Entry historyiIntegrated into UniProtKB/TrEMBL: June 26, 2013
Last sequence update: June 26, 2013
Last modified: October 25, 2017
This is version 32 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteomeImported