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Protein

Intron-encoded DNA endonuclease aI5 alpha

Gene

AI5_ALPHA

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mitochondrial DNA endonuclease involved in intron homing. It introduces a specific double-strand break in the DNA of the COX1 gene and thus mediates the insertion of an intron, containing its own coding sequence (group I intron), into an intronless gene. Recognizes with limited specificity and cleaves the sequence 5'-ATCTTCTCTTGATTAGCCCTGATCTACGG-3' after the nucleotide sequence ATTA leaving a four-base overhang and a 3'-OH.By similarity2 Publications

Cofactori

Mg2+1 Publication

pH dependencei

Optimum pH is 9.5.1 Publication

GO - Molecular functioni

  • cytochrome-c oxidase activity Source: InterPro
  • endonuclease activity Source: SGD
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processIntron homing, mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-34376-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Intron-encoded DNA endonuclease aI5 alpha
Alternative name(s):
DNA endonuclease I-SceIV
Cleaved into the following 2 chains:
Alternative name(s):
Intron-encoded endonuclease I-SceIV
Gene namesi
Name:AI5_ALPHA
Synonyms:I-SCEIV1 Publication
Ordered Locus Names:Q0070
Encoded oniMitochondrion
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Mitochondrion

Organism-specific databases

EuPathDBiFungiDB:Q0070
SGDiS000007265 AI5_ALPHA

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei16 – 36HelicalSequence analysisAdd BLAST21
Transmembranei57 – 77HelicalSequence analysisAdd BLAST21
Transmembranei101 – 121HelicalSequence analysisAdd BLAST21
Transmembranei147 – 167HelicalSequence analysisAdd BLAST21
Transmembranei182 – 202HelicalSequence analysisAdd BLAST21
Transmembranei235 – 255HelicalSequence analysisAdd BLAST21
Transmembranei267 – 287HelicalSequence analysisAdd BLAST21
Transmembranei305 – 325HelicalSequence analysisAdd BLAST21
Transmembranei417 – 437HelicalSequence analysisAdd BLAST21
Transmembranei464 – 483HelicalSequence analysisAdd BLAST20

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000270984? – 630DNA endonuclease aI5 alpha
ChainiPRO_00002709831 – ?Truncated non-functional cytochrome oxidase 1

Post-translational modificationi

The mature protein may arise from proteolytic cleavage of an in-frame translation of some COX1 exons plus the intron containing the aI5_alpha open reading frame.2 Publications

Proteomic databases

PaxDbiQ9ZZX1
PRIDEiQ9ZZX1

Interactioni

Subunit structurei

Heterodimer of a 32 kDa catalytic subunit and a larger 60 kDa polypeptide subunit.1 Publication

Protein-protein interaction databases

BioGridi34789, 2 interactors
IntActiQ9ZZX1, 9 interactors
STRINGi4932.Q0070

Structurei

3D structure databases

ProteinModelPortaliQ9ZZX1
SMRiQ9ZZX1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 324COX1 exons encodedSequence analysisAdd BLAST324
Regioni325 – 630COX1 intron aI5_alpha encodedAdd BLAST306

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi333 – 390Asn-richSequence analysisAdd BLAST58

Sequence similaritiesi

In the N-terminal section; belongs to the heme-copper respiratory oxidase family.Sequence analysis
In the C-terminal section; belongs to the LAGLIDADG endonuclease family.Sequence analysis

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00840000130858
InParanoidiQ9ZZX1
OrthoDBiEOG092C2KU4

Family and domain databases

Gene3Di1.20.210.10, 1 hit
3.10.28.10, 1 hit
InterProiView protein in InterPro
IPR023616 Cyt_c_oxase-like_su1_dom
IPR036927 Cyt_c_oxase-like_su1_sf
IPR000883 Cyt_C_Oxase_1
IPR023615 Cyt_c_Oxase_su1_BS
IPR027434 Homing_endonucl
IPR004860 LAGLIDADG_2
PANTHERiPTHR10422 PTHR10422, 1 hit
PfamiView protein in Pfam
PF00115 COX1, 1 hit
PF03161 LAGLIDADG_2, 1 hit
PRINTSiPR01165 CYCOXIDASEI
SUPFAMiSSF55608 SSF55608, 1 hit
SSF81442 SSF81442, 1 hit
PROSITEiView protein in PROSITE
PS50855 COX1, 1 hit
PS00077 COX1_CUB, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ZZX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQRWLYSTN AKDIAVLYFM LAIFSGMAGT AMSLIIRLEL AAPGSQYLHG
60 70 80 90 100
NSQLFNVLVV GHAVLMIFFL VMPALIGGFG NYLLPLMIGA TDTAFPRINN
110 120 130 140 150
IAFWVLPMGL VCLVTSTLVE SGAGTGWTVY PPLSSIQAHS GPSVDLAIFA
160 170 180 190 200
LHLTSISSLL GAINFIVTTL NMRTNGMTMH KLPLFVWSIF ITAFLLLLSL
210 220 230 240 250
PVLSAGITML LLDRNFNTSF FEVSGGGDPI LYEHLFWFFG HPEVYILIIP
260 270 280 290 300
GFGIISHVVS TYSKKPVFGE ISMVYAMASI GLLGFLVWSH HMYIVGLDAD
310 320 330 340 350
TRAYFTSATM IIAIPTGIKI FSWLMNPFSK DKNKNKNKKL IRNYQKMNNN
360 370 380 390 400
NMMKTYLNNN NMIMMNMYKG NLYDIYPRSN RNYIQPNNIN KELVVYGYNL
410 420 430 440 450
ESCVGMPTYT NIVKHMVGIP NNILYIMTGI LLTDGWIDYT SKKDLDKKTI
460 470 480 490 500
MEINCRFRLK QSMIHSEYLM YVFMLLSHYC MSYPKMKIAK VKGKSYNQLE
510 520 530 540 550
FYTRSLPCFT ILRYMFYNGR VKIVPNNLYD LLNYESLAHM IMCDGSFVKG
560 570 580 590 600
GGLYLNLQSF TTKELIFIMN ILKIKFNLNC TLHKSRNKYT IYMRVESVKR
610 620 630
LFPMIYKYIL PSMRYKFDIM LWQKKYNMIN
Length:630
Mass (Da):72,165
Last modified:May 1, 1999 - v1
Checksum:i6EA9AA02CD2A6C16
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KP263414 Genomic DNA Translation: AIZ98882.1
PIRiS78650
RefSeqiNP_009306.1, NC_001224.1

Genome annotation databases

EnsemblFungiiQ0070; Q0070; Q0070
GeneIDi854597
KEGGisce:Q0070

Similar proteinsi

Entry informationi

Entry nameiSCE4_YEAST
AccessioniPrimary (citable) accession number: Q9ZZX1
Secondary accession number(s): A0A0A7NYD1, Q8W770
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: May 1, 1999
Last modified: March 28, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health