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Protein

Intron-encoded DNA endonuclease aI5 alpha

Gene

AI5_ALPHA

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial DNA endonuclease involved in intron homing. It introduces a specific double-strand break in the DNA of the COX1 gene and thus mediates the insertion of an intron, containing its own coding sequence (group I intron), into an intronless gene. Recognizes with limited specificity and cleaves the sequence 5'-ATCTTCTCTTGATTAGCCCTGATCTACGG-3' after the nucleotide sequence ATTA leaving a four-base overhang and a 3'-OH.By similarity2 Publications

Cofactori

Mg2+1 Publication

pH dependencei

Optimum pH is 9.5.1 Publication

GO - Molecular functioni

  • cytochrome-c oxidase activity Source: InterPro
  • endonuclease activity Source: SGD
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro

GO - Biological processi

  • aerobic respiration Source: InterPro
  • movement of group I intron Source: SGD
  • mRNA processing Source: UniProtKB-KW
  • RNA splicing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Intron homing, mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-34376-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Intron-encoded DNA endonuclease aI5 alpha
Alternative name(s):
DNA endonuclease I-SceIV
Cleaved into the following 2 chains:
Alternative name(s):
Intron-encoded endonuclease I-SceIV
Gene namesi
Name:AI5_ALPHA
Synonyms:I-SCEIV1 Publication
Ordered Locus Names:Q0070
Encoded oniMitochondrion
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Mitochondrion

Organism-specific databases

EuPathDBiFungiDB:Q0070.
SGDiS000007265. AI5_ALPHA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei16 – 3621HelicalSequence analysisAdd
BLAST
Transmembranei57 – 7721HelicalSequence analysisAdd
BLAST
Transmembranei101 – 12121HelicalSequence analysisAdd
BLAST
Transmembranei147 – 16721HelicalSequence analysisAdd
BLAST
Transmembranei182 – 20221HelicalSequence analysisAdd
BLAST
Transmembranei235 – 25521HelicalSequence analysisAdd
BLAST
Transmembranei267 – 28721HelicalSequence analysisAdd
BLAST
Transmembranei305 – 32521HelicalSequence analysisAdd
BLAST
Transmembranei417 – 43721HelicalSequence analysisAdd
BLAST
Transmembranei464 – 48320HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 630DNA endonuclease aI5 alphaPRO_0000270984
Chaini1 – ?Truncated non-functional cytochrome oxidase 1PRO_0000270983

Post-translational modificationi

The mature protein may arise from proteolytic cleavage of an in-frame translation of some COX1 exons plus the intron containing the aI5_alpha open reading frame.2 Publications

Interactioni

Subunit structurei

Heterodimer of a 32 kDa catalytic subunit and a larger 60 kDa polypeptide subunit.1 Publication

Protein-protein interaction databases

BioGridi34789. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9ZZX1.
SMRiQ9ZZX1. Positions 2-324, 509-617.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 324324COX1 exons encodedSequence analysisAdd
BLAST
Regioni325 – 630306COX1 intron aI5_alpha encodedAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi333 – 39058Asn-richSequence analysisAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the heme-copper respiratory oxidase family.Sequence analysis
In the C-terminal section; belongs to the LAGLIDADG endonuclease family.Sequence analysis

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00840000130858.
InParanoidiQ9ZZX1.
OrthoDBiEOG092C2KU4.

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
3.10.28.10. 2 hits.
InterProiIPR000883. COX1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
IPR027434. Homing_endonucl.
IPR004860. LAGLIDADG_2.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
PF03161. LAGLIDADG_2. 1 hit.
[Graphical view]
PRINTSiPR01165. CYCOXIDASEI.
SUPFAMiSSF55608. SSF55608. 1 hit.
SSF81442. SSF81442. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ZZX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQRWLYSTN AKDIAVLYFM LAIFSGMAGT AMSLIIRLEL AAPGSQYLHG
60 70 80 90 100
NSQLFNVLVV GHAVLMIFFL VMPALIGGFG NYLLPLMIGA TDTAFPRINN
110 120 130 140 150
IAFWVLPMGL VCLVTSTLVE SGAGTGWTVY PPLSSIQAHS GPSVDLAIFA
160 170 180 190 200
LHLTSISSLL GAINFIVTTL NMRTNGMTMH KLPLFVWSIF ITAFLLLLSL
210 220 230 240 250
PVLSAGITML LLDRNFNTSF FEVSGGGDPI LYEHLFWFFG HPEVYILIIP
260 270 280 290 300
GFGIISHVVS TYSKKPVFGE ISMVYAMASI GLLGFLVWSH HMYIVGLDAD
310 320 330 340 350
TRAYFTSATM IIAIPTGIKI FSWLMNPFSK DKNKNKNKKL IRNYQKMNNN
360 370 380 390 400
NMMKTYLNNN NMIMMNMYKG NLYDIYPRSN RNYIQPNNIN KELVVYGYNL
410 420 430 440 450
ESCVGMPTYT NIVKHMVGIP NNILYIMTGI LLTDGWIDYT SKKDLDKKTI
460 470 480 490 500
MEINCRFRLK QSMIHSEYLM YVFMLLSHYC MSYPKMKIAK VKGKSYNQLE
510 520 530 540 550
FYTRSLPCFT ILRYMFYNGR VKIVPNNLYD LLNYESLAHM IMCDGSFVKG
560 570 580 590 600
GGLYLNLQSF TTKELIFIMN ILKIKFNLNC TLHKSRNKYT IYMRVESVKR
610 620 630
LFPMIYKYIL PSMRYKFDIM LWQKKYNMIN
Length:630
Mass (Da):72,165
Last modified:May 1, 1999 - v1
Checksum:i6EA9AA02CD2A6C16
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KP263414 Genomic DNA. Translation: AIZ98882.1.
PIRiS78650.
RefSeqiNP_009306.1. NC_001224.1.

Genome annotation databases

EnsemblFungiiQ0070; Q0070; Q0070.
GeneIDi854597.
KEGGisce:Q0070.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KP263414 Genomic DNA. Translation: AIZ98882.1.
PIRiS78650.
RefSeqiNP_009306.1. NC_001224.1.

3D structure databases

ProteinModelPortaliQ9ZZX1.
SMRiQ9ZZX1. Positions 2-324, 509-617.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34789. 2 interactions.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiQ0070; Q0070; Q0070.
GeneIDi854597.
KEGGisce:Q0070.

Organism-specific databases

EuPathDBiFungiDB:Q0070.
SGDiS000007265. AI5_ALPHA.

Phylogenomic databases

GeneTreeiENSGT00840000130858.
InParanoidiQ9ZZX1.
OrthoDBiEOG092C2KU4.

Enzyme and pathway databases

BioCyciYEAST:G3O-34376-MONOMER.

Miscellaneous databases

PROiQ9ZZX1.

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
3.10.28.10. 2 hits.
InterProiIPR000883. COX1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
IPR027434. Homing_endonucl.
IPR004860. LAGLIDADG_2.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
PF03161. LAGLIDADG_2. 1 hit.
[Graphical view]
PRINTSiPR01165. CYCOXIDASEI.
SUPFAMiSSF55608. SSF55608. 1 hit.
SSF81442. SSF81442. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSCE4_YEAST
AccessioniPrimary (citable) accession number: Q9ZZX1
Secondary accession number(s): A0A0A7NYD1, Q8W770
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: May 1, 1999
Last modified: September 7, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.