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Protein

Lipoyl synthase, mitochondrial

Gene

LIP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation1 Publication

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Putative lipoyltransferase-like protein, chloroplastic (At1g47578), Octanoyltransferase (LIP2)
  2. Lipoyl synthase, chloroplastic (LIP1P), Lipoyl synthase, chloroplastic (LIP1P), Lipoyl synthase, mitochondrial (LIP1), Lipoyl synthase, mitochondrial (LIP1)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi103Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi108Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi114Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi134Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi138Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi141Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • glycine catabolic process Source: TAIR
  • protein lipoylation Source: GO_Central

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.8.1.8 399
UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
Name:LIP1UniRule annotation
Ordered Locus Names:At2g20860
ORF Names:F5H14.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G20860
TAIRilocus:2051384 AT2G20860

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 19MitochondrionUniRule annotationAdd BLAST19
ChainiPRO_000039884420 – 374Lipoyl synthase, mitochondrialAdd BLAST355

Proteomic databases

PaxDbiQ9ZWT1
PRIDEiQ9ZWT1

Expressioni

Tissue specificityi

Expressed in leaves and flowers, but not in roots.1 Publication

Gene expression databases

ExpressionAtlasiQ9ZWT1 baseline and differential
GenevisibleiQ9ZWT1 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G20860.1

Structurei

3D structure databases

ProteinModelPortaliQ9ZWT1
SMRiQ9ZWT1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2672 Eukaryota
COG0320 LUCA
HOGENOMiHOG000235998
KOiK03644
OMAiGRCPNRG
OrthoDBiEOG09360EMI
PhylomeDBiQ9ZWT1

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
MF_03128 Lipoyl_synth_plantM, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR027527 Lipoyl_synth_mt
IPR007197 rSAM
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ZWT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHSRSALLYR FLRPASRCFS SSSAVTPVTV TQSPKSLEAL RARLANESPS
60 70 80 90 100
LTDFIHGDTY SVEVGTKKKP LPKPKWMKES IPGGERYVQI KKKLRDLKLH
110 120 130 140 150
TVCEEAKCPN LGECWSGGET GTATATIMIL GDTCTRGCRF CNVKTSRTPP
160 170 180 190 200
PPDPNEPNNV AEAIASWGVD YVVITSVDRD DLPDQGSGHF AETVQRLKFL
210 220 230 240 250
KPEMLIEALV PDFRGDGGCV EKVSKSGLDV LAHNIETVEE LQSFVRDHRA
260 270 280 290 300
NFKQSLDVLR MAKEYAPAGT LTKTSVMLGC GETPDQVVKT MEKVRAAGVD
310 320 330 340 350
VMTFGQYMRP SKRHMPVAEY VTPDAFERYR LLGMEMGFRY VASGPMVRSS
360 370
YKAGEYYIKS MIEADRVASP STSP
Length:374
Mass (Da):41,344
Last modified:May 1, 1999 - v1
Checksum:i9527359AA5E9AF6A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007987 mRNA Translation: BAA34701.1
AC006234 Genomic DNA Translation: AAD20909.1
CP002685 Genomic DNA Translation: AEC07088.1
CP002685 Genomic DNA Translation: ANM61393.1
CP002685 Genomic DNA Translation: ANM61394.1
AY035143 mRNA Translation: AAK59647.1
AF424583 mRNA Translation: AAL11577.1
AY059077 mRNA Translation: AAL15183.1
PIRiT44259
RefSeqiNP_001318257.1, NM_001335714.1
NP_001323612.1, NM_001335715.1
NP_179682.1, NM_127655.4
UniGeneiAt.21247

Genome annotation databases

EnsemblPlantsiAT2G20860.1; AT2G20860.1; AT2G20860
AT2G20860.2; AT2G20860.2; AT2G20860
AT2G20860.3; AT2G20860.3; AT2G20860
GeneIDi816619
GrameneiAT2G20860.1; AT2G20860.1; AT2G20860
AT2G20860.2; AT2G20860.2; AT2G20860
AT2G20860.3; AT2G20860.3; AT2G20860
KEGGiath:AT2G20860

Similar proteinsi

Entry informationi

Entry nameiLIAS_ARATH
AccessioniPrimary (citable) accession number: Q9ZWT1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 1, 1999
Last modified: May 23, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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