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Q9ZWT1

- LIAS_ARATH

UniProt

Q9ZWT1 - LIAS_ARATH

Protein

Lipoyl synthase, mitochondrial

Gene

LIP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.1 PublicationUniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi103 – 1031Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi108 – 1081Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi114 – 1141Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi134 – 1341Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi138 – 1381Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi141 – 1411Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: TAIR
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glycine catabolic process Source: TAIR
    2. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    Name:LIP1UniRule annotation
    Ordered Locus Names:At2g20860
    ORF Names:F5H14.17
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G20860.

    Subcellular locationi

    Mitochondrion 1 PublicationUniRule annotation

    GO - Cellular componenti

    1. mitochondrial matrix Source: TAIR

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 1919MitochondrionUniRule annotationAdd
    BLAST
    Chaini20 – 374355Lipoyl synthase, mitochondrialPRO_0000398844Add
    BLAST

    Proteomic databases

    PaxDbiQ9ZWT1.
    PRIDEiQ9ZWT1.

    Expressioni

    Tissue specificityi

    Expressed in leaves and flowers, but not in roots.1 Publication

    Gene expression databases

    GenevestigatoriQ9ZWT1.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G20860.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ZWT1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235998.
    InParanoidiQ9ZWT1.
    KOiK03644.
    OMAiHTICQEA.
    PhylomeDBiQ9ZWT1.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    MF_03128. Lipoyl_synth_plantM.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR027527. Lipoyl_synth_mt.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9ZWT1-1 [UniParc]FASTAAdd to Basket

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    MHSRSALLYR FLRPASRCFS SSSAVTPVTV TQSPKSLEAL RARLANESPS    50
    LTDFIHGDTY SVEVGTKKKP LPKPKWMKES IPGGERYVQI KKKLRDLKLH 100
    TVCEEAKCPN LGECWSGGET GTATATIMIL GDTCTRGCRF CNVKTSRTPP 150
    PPDPNEPNNV AEAIASWGVD YVVITSVDRD DLPDQGSGHF AETVQRLKFL 200
    KPEMLIEALV PDFRGDGGCV EKVSKSGLDV LAHNIETVEE LQSFVRDHRA 250
    NFKQSLDVLR MAKEYAPAGT LTKTSVMLGC GETPDQVVKT MEKVRAAGVD 300
    VMTFGQYMRP SKRHMPVAEY VTPDAFERYR LLGMEMGFRY VASGPMVRSS 350
    YKAGEYYIKS MIEADRVASP STSP 374
    Length:374
    Mass (Da):41,344
    Last modified:May 1, 1999 - v1
    Checksum:i9527359AA5E9AF6A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB007987 mRNA. Translation: BAA34701.1.
    AC006234 Genomic DNA. Translation: AAD20909.1.
    CP002685 Genomic DNA. Translation: AEC07088.1.
    AY035143 mRNA. Translation: AAK59647.1.
    AF424583 mRNA. Translation: AAL11577.1.
    AY059077 mRNA. Translation: AAL15183.1.
    PIRiT44259.
    RefSeqiNP_179682.1. NM_127655.3.
    UniGeneiAt.21247.

    Genome annotation databases

    EnsemblPlantsiAT2G20860.1; AT2G20860.1; AT2G20860.
    GeneIDi816619.
    KEGGiath:AT2G20860.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB007987 mRNA. Translation: BAA34701.1 .
    AC006234 Genomic DNA. Translation: AAD20909.1 .
    CP002685 Genomic DNA. Translation: AEC07088.1 .
    AY035143 mRNA. Translation: AAK59647.1 .
    AF424583 mRNA. Translation: AAL11577.1 .
    AY059077 mRNA. Translation: AAL15183.1 .
    PIRi T44259.
    RefSeqi NP_179682.1. NM_127655.3.
    UniGenei At.21247.

    3D structure databases

    ProteinModelPortali Q9ZWT1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G20860.1-P.

    Proteomic databases

    PaxDbi Q9ZWT1.
    PRIDEi Q9ZWT1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G20860.1 ; AT2G20860.1 ; AT2G20860 .
    GeneIDi 816619.
    KEGGi ath:AT2G20860.

    Organism-specific databases

    TAIRi AT2G20860.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235998.
    InParanoidi Q9ZWT1.
    KOi K03644.
    OMAi HTICQEA.
    PhylomeDBi Q9ZWT1.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Gene expression databases

    Genevestigatori Q9ZWT1.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    MF_03128. Lipoyl_synth_plantM.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR027527. Lipoyl_synth_mt.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Biosynthesis of lipoic acid in Arabidopsis: cloning and characterization of the cDNA for lipoic acid synthase."
      Yasuno R., Wada H.
      Plant Physiol. 118:935-943(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiLIAS_ARATH
    AccessioniPrimary (citable) accession number: Q9ZWT1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3