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Protein

Flavonol synthase/flavanone 3-hydroxylase

Gene

FLS

Organism
Citrus unshiu (Satsuma mandarin) (Citrus nobilis var. unshiu)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of flavonols from dihydroflavonols. It can act on dihydrokaempferol to produce kaempferol, on dihydroquercetin to produce quercitin and on dihydromyricetin to produce myricetin (By similarity).By similarity1 Publication

Catalytic activityi

A dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O.By similarity
A (2S)-flavan-4-one + 2-oxoglutarate + O2 = a (2R,3R)-dihydroflavonol + succinate + CO2.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • L-ascorbateBy similarityNote: Binds 1 ascorbate molecule per subunit.By similarity

Pathwayi: flavonoid biosynthesis

This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.
View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi221IronPROSITE-ProRule annotation1
Metal bindingi223IronPROSITE-ProRule annotation1
Metal bindingi277IronPROSITE-ProRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processFlavonoid biosynthesis
LigandIron, Metal-binding, Vitamin C

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18283.
BRENDAi1.14.11.23. 1428.
UniPathwayiUPA00154.

Names & Taxonomyi

Protein namesi
Recommended name:
Flavonol synthase/flavanone 3-hydroxylase (EC:1.14.11.23By similarity, EC:1.14.11.9By similarity)
Alternative name(s):
CitFLS
Short name:
FLS
Gene namesi
Name:FLS
OrganismiCitrus unshiu (Satsuma mandarin) (Citrus nobilis var. unshiu)
Taxonomic identifieri55188 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsSapindalesRutaceaeAurantioideaeCitrus

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi68G → A: Reduces activity 95%. 1 Publication1
Mutagenesisi68G → P: Results in complete loss of activity. 1 Publication1
Mutagenesisi207P → G: No effect. 1
Mutagenesisi261G → A: Reduces activity 95%. 1 Publication1
Mutagenesisi261G → P: Results in complete loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000672921 – 335Flavonol synthase/flavanone 3-hydroxylaseAdd BLAST335

Expressioni

Tissue specificityi

In the juice sacs/segment epidermis (edible part) at the early developmental stage.

Developmental stagei

Increases in the peel during fruit maturation.

Structurei

3D structure databases

ProteinModelPortaliQ9ZWQ9.
SMRiQ9ZWQ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini196 – 296Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST101

Sequence similaritiesi

Phylogenomic databases

KOiK05278.

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiView protein in InterPro
IPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
PfamiView protein in Pfam
PF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
PROSITEiView protein in PROSITE
PS51471. FE2OG_OXY. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9ZWQ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVERVQAIA SLSHSNGTIP AEFIRPEKEQ PASTTYHGPA PEIPTIDLDD
60 70 80 90 100
PVQDRLVRSI AEASREWGIF QVTNHGIPSD LICKLQAVGK EFFELPQEEK
110 120 130 140 150
EVYSRPADAK DVQGYGTKLQ KEVEGKKSWV DHLFHRVWPP SSINYRFWPK
160 170 180 190 200
NPPSYRAVNE EYAKYMREVV DKLFTYLSLG LGVEGGVLKE AAGGDDIEYM
210 220 230 240 250
LKINYYPPCP RPDLALGVVA HTDLSALTVL VPNEVPGLQV FKDDRWIDAK
260 270 280 290 300
YIPNALVIHI GDQIEILSNG KYKAVLHRTT VNKDKTRMSW PVFLEPPADT
310 320 330
VVGPLPQLVD DENPPKYKAK KFKDYSYCKL NKLPQ
Length:335
Mass (Da):37,899
Last modified:May 1, 1999 - v1
Checksum:i157E1AFA4C47564B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011796 mRNA. Translation: BAA36554.1.

Genome annotation databases

KEGGiag:BAA36554.

Similar proteinsi

Entry informationi

Entry nameiFLS_CITUN
AccessioniPrimary (citable) accession number: Q9ZWQ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: May 1, 1999
Last modified: February 15, 2017
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families