Q9ZWJ9 (ARR2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Two-component response regulator ARR2

Alternative name(s):
Receiver-like protein 5

Gene names

Name:	ARR2
Synonyms:	ARP5
Ordered Locus Names:	At4g16110
ORF Names:	dl4095w, FCAALL.297

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	664 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]GATT-3'. Functions as a response regulator involved in His-to-Asp phosphorelay signal transduction system. Phosphorylation of the Asp residue in the receiver domain activates the ability of the protein to promote the transcription of target genes. Could directly activate some type-A response regulators in response to cytokinins. Involved in the expression of nuclear genes for components of mitochondrial complex I. Promotes cytokinin-mediated leaf longevity. Involved in the ethylene signaling pathway in an ETR1-dependent manner and in the cytokinin signaling pathway. Ref.2 Ref.3 Ref.9 Ref.12
Subunit structure	Binds the target DNA as a monomer By similarity. Interacts with histidine-containing phosphotransfer proteins. Ref.2 Ref.10
Subcellular location	Nucleus Ref.3 Ref.8 Ref.9.
Tissue specificity	Detected in the whole plant. Predominantly expressed in pollen. Ref.1 Ref.2 Ref.11
Post-translational modification	Two-component system major event consists of a His-to-Asp phosphorelay between a sensor histidine kinase (HK) and a response regulator (RR). In plants, the His-to-Asp phosphorelay involves an additional intermediate named Histidine-containing phosphotransfer protein (HPt). This multistep phosphorelay consists of a His-Asp-His-Asp sequential transfer of a phosphate group between first an His and an Asp of the HK protein, followed by the transfer to a conserved His of the HPt protein and finally the transfer to an Asp in the receiver domain of the RR protein. Phosphorylated in response to cytokinin mediated by AHK3. Ref.3 Ref.12
Disruption phenotype	Retarded growth and development, and early flowering. Reduced responses to ethylene and cytokinin. Ref.3
Sequence similarities	Belongs to the ARR family. Type-B subfamily. Contains 1 Myb-like GARP DNA-binding domain. Contains 1 response regulatory domain.
Sequence caution	The sequence CAA06433.1 differs from that shown. Reason: Artifacts of PCR amplification. The sequence CAB10390.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB78653.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cytokinin signaling pathway Ethylene signaling pathway Transcription Transcription regulation Two-component regulatory system
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cytokinin mediated signaling pathway Traceable author statement. Source: TAIR ethylene mediated signaling pathway Inferred from mutant phenotype. Source: TAIR leaf senescence Inferred from mutant phenotype Ref.12. Source: UniProtKB regulation of stomatal movement Inferred from mutant phenotype. Source: TAIR transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from direct assay Ref.8Ref.2. Source: TAIR
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.8. Source: TAIR two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AHP1	Q9ZNV9	4	EBI-1101028,EBI-1100673
AHP2	Q9ZNV8	4	EBI-1101028,EBI-1100687

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 664	664	Two-component response regulator ARR2		PRO_0000132294
Regions
	Domain	29 – 144	116	Response regulatory
	DNA binding	218 – 268	51	Myb-like GARP Ref.8
	Motif	215 – 218	4	Nuclear localization signal Potential
	Compositional bias	279 – 664	386	Gln-rich
Amino acid modifications
	Modified residue	80	1	4-aspartylphosphate
Natural variations
	Natural variant	62	1	S → I in strain: cv. Landsberg erecta. Ref.3
	Natural variant	283	1	N → I in strain: cv. Landsberg erecta. Ref.3
	Natural variant	385	1	L → V in strain: cv. Landsberg erecta. Ref.3
	Natural variant	655	1	D → G in strain: cv. Landsberg erecta. Ref.3
Experimental info
	Mutagenesis	80	1	D → N: Impaired cytokinin-mediated and ethylene-mediated phosphorylation, severe pleiotropic aberrations in growth and development. Ref.3 Ref.12
	Sequence conflict	35	1	D → G in BAD43141. Ref.7
	Sequence conflict	518	1	H → R in BAD43141. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q9ZWJ9 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: F930558EB55549F4	FASTA	664	72,578
	10 20 30 40 50 60 MVNPGHGRGP DSGTAAGGSN SDPFPANLRV LVVDDDPTCL MILERMLMTC LYRVTKCNRA 70 80 90 100 110 120 ESALSLLRKN KNGFDIVISD VHMPDMDGFK LLEHVGLEMD LPVIMMSADD SKSVVLKGVT 130 140 150 160 170 180 HGAVDYLIKP VRIEALKNIW QHVVRKKRNE WNVSEHSGGS IEDTGGDRDR QQQHREDADN 190 200 210 220 230 240 NSSSVNEGNG RSSRKRKEEE VDDQGDDKED SSSLKKPRVV WSVELHQQFV AAVNQLGVDK 250 260 270 280 290 300 AVPKKILEMM NVPGLTRENV ASHLQKYRIY LRRLGGVSQH QGNMNHSFMT GQDQSFGPLS 310 320 330 340 350 360 SLNGFDLQSL AVTGQLPPQS LAQLQAAGLG RPTLAKPGMS VSPLVDQRSI FNFENPKIRF 370 380 390 400 410 420 GDGHGQTMNN GNLLHGVPTG SHMRLRPGQN VQSSGMMLPV ADQLPRGGPS MLPSLGQQPI 430 440 450 460 470 480 LSSSVSRRSD LTGALAVRNS IPETNSRVLP TTHSVFNNFP ADLPRSSFPL ASAPGISVPV 490 500 510 520 530 540 SVSYQEEVNS SDAKGGSSAA TAGFGNPSYD IFNDFPQHQQ HNKNISNKLN DWDLRNMGLV 550 560 570 580 590 600 FSSNQDAATA TATAAFSTSE AYSSSSTQRK RRETDATVVG EHGQNLQSPS RNLYHLNHVF 610 620 630 640 650 660 MDGGSVRVKS ERVAETVTCP PANTLFHEQY NQEDLMSAFL KQEGIPSVDN EFEFDGYSID NIQV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two-component response regulators from Arabidopsis thaliana contain a putative DNA-binding motif." Sakai H., Aoyama T., Bono H., Oka A. Plant Cell Physiol. 39:1232-1239(1998) [PubMed: 9891419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY. Strain: cv. Columbia.
[2]	"The response regulator ARR2: a pollen-specific transcription factor involved in the expression of nuclear genes for components of mitochondrial complex I in Arabidopsis." Lohrmann J., Sweere U., Zabaleta E., Baeurle I., Keitel C., Kozma-Bognar L., Brennicke A., Schaefer E., Kudla J., Harter K. Mol. Genet. Genomics 265:2-13(2001) [PubMed: 11370868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INTERACTION. Strain: cv. Columbia. Tissue: Leaf.
[3]	"The response regulator 2 mediates ethylene signalling and hormone signal integration in Arabidopsis." Hass C., Lohrmann J., Albrecht V., Sweere U., Hummel F., Yoo S.D., Hwang I., Zhu T., Schaefer E., Kudla J., Harter K. EMBO J. 23:3290-3302(2004) [PubMed: 15282545] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANTS ILE-62; ILE-283; VAL-385 AND GLY-655, FUNCTION, DISRUPTION PHENOTYPE, PHOSPHORYLATION AT ASP-80, MUTAGENESIS OF ASP-80, SUBCELLULAR LOCATION. Strain: cv. Columbia and cv. Landsberg erecta.
[4]	"Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana." Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C., Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E. , Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R., De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M., Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M., Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A., Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D., Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A., Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S., Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G., Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N. Nature 391:485-488(1998) [PubMed: 9461215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[6]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[7]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[8]	"Arabidopsis ARR1 and ARR2 response regulators operate as transcriptional activators." Sakai H., Aoyama T., Oka A. Plant J. 24:703-711(2000) [PubMed: 11135105] [Abstract] Cited for: DNA-BINDING SPECIFICITY, SUBCELLULAR LOCATION.
[9]	"Two-component circuitry in Arabidopsis cytokinin signal transduction." Hwang I., Sheen J. Nature 413:383-389(2001) [PubMed: 11574878] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[10]	"Comparative studies of the AHP histidine-containing phosphotransmitters implicated in His-to-Asp phosphorelay in Arabidopsis thaliana." Tanaka Y., Suzuki T., Yamashino T., Mizuno T. Biosci. Biotechnol. Biochem. 68:462-465(2004) [PubMed: 14981318] [Abstract] Cited for: INTERACTION.
[11]	"Type-B response regulators display overlapping expression patterns in Arabidopsis." Mason M.G., Li J., Mathews D.E., Kieber J.J., Schaller G.E. Plant Physiol. 135:927-937(2004) [PubMed: 15173562] [Abstract] Cited for: TISSUE SPECIFICITY.
[12]	"Cytokinin-mediated control of leaf longevity by AHK3 through phosphorylation of ARR2 in Arabidopsis." Kim H.J., Ryu H., Hong S.H., Woo H.R., Lim P.O., Lee I.C., Sheen J., Nam H.G., Hwang I. Proc. Natl. Acad. Sci. U.S.A. 103:814-819(2006) [PubMed: 16407152] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ASP-80, PHOSPHORYLATION AT ASP-80.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB016472 Genomic DNA. Translation: BAA74527.1. AJ005196 mRNA. Translation: CAA06433.1. Sequence problems. DQ473518 Genomic DNA. Translation: ABF47278.1. DQ473519 mRNA. Translation: ABF47279.1. DQ473520 Genomic DNA. Translation: ABF19058.1. DQ473521 mRNA. Translation: ABF19059.1. Z97340 Genomic DNA. Translation: CAB10390.1. Sequence problems. AL161543 Genomic DNA. Translation: CAB78653.1. Sequence problems. CP002687 Genomic DNA. Translation: AEE83694.1. AK175378 mRNA. Translation: BAD43141.1. AK175737 mRNA. Translation: BAD43500.1. AK175959 mRNA. Translation: BAD43722.1. AK176614 mRNA. Translation: BAD44377.1.
IPI	IPI00518324.
PIR	D71427. T51247.
RefSeq	NP_193346.4. NM_117704.6.
UniGene	At.21955.
3D structure databases
ProteinModelPortal	Q9ZWJ9.
SMR	Q9ZWJ9. Positions 29-145, 212-274.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9ZWJ9. 8 interactions.
STRING	Q9ZWJ9.
Proteomic databases
PRIDE	Q9ZWJ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G16110.1; AT4G16110.1; AT4G16110.
GeneID	827297.
GenomeReviews	Gene locus AT4G16110 in contig CT486007_GR.
KEGG	ath:AT4G16110.
Organism-specific databases
GeneFarm	3921. 401.
TAIR	At4g16110.
Phylogenomic databases
eggNOG	KOG1601.
GeneTree	EPGT00070000028006.
HOGENOM	HBG748984.
InParanoid	Q9ZWJ9.
PhylomeDB	Q9ZWJ9.
ProtClustDB	CLSN2680844.
Gene expression databases
Genevestigator	Q9ZWJ9.
GermOnline	AT4G16110. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR011006. CheY-like_superfamily. IPR009057. Homeodomain-like. IPR012287. Homeodomain-rel. IPR006447. Myb-like+DNA-bd_dom_SHAQKYF. IPR014778. Myb_DNA-bd. IPR017053. Response_reg_B-typ_pln. IPR001789. Sig_transdc_resp-reg_receiver. IPR017930. Tscrpt_reg_HTH_Myb-type. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
KO	K14491.
Pfam	PF00249. Myb_DNA-binding. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF036392. RR_ARR_type-B. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52172. CheY_like. 1 hit. SSF46689. Homeodomain_like. 1 hit.
TIGRFAMs	TIGR01557. Myb_SHAQKYF. 1 hit.
PROSITE	PS51294. HTH_MYB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARR2_ARATH

Accession

Primary (citable) accession number: Q9ZWJ9
Secondary accession number(s): C3PTB4 , O23460, Q680A8, Q682I9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	May 1, 1999
Last modified:	December 14, 2011
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program