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Protein

Serine/threonine-protein kinase BRI1-like 1

Gene

BRL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor with a serine/threonine-protein kinase activity. Regulates, in response to brassinosteroid binding, a signaling cascade involved in plant development. Binds brassinolide. May be involved in cell growth and vascular differentiation.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei887ATPPROSITE-ProRule annotation1
Active sitei987Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi865 – 873ATPPROSITE-ProRule annotation9

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Lipid-binding, Nucleotide-binding, Steroid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase BRI1-like 1 (EC:2.7.11.1)
Alternative name(s):
BRASSINOSTEROID INSENSITIVE 1-like protein 1
Gene namesi
Name:BRL1
Ordered Locus Names:At1g55610
ORF Names:F20N2.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G55610.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 776ExtracellularSequence analysisAdd BLAST755
Transmembranei777 – 797HelicalSequence analysisAdd BLAST21
Topological domaini798 – 1166CytoplasmicSequence analysisAdd BLAST369

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000002430822 – 1166Serine/threonine-protein kinase BRI1-like 1Add BLAST1145

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi33N-linked (GlcNAc...)Sequence analysis1
Glycosylationi97N-linked (GlcNAc...)Sequence analysis1
Glycosylationi157N-linked (GlcNAc...)Sequence analysis1
Glycosylationi212N-linked (GlcNAc...)Sequence analysis1
Glycosylationi227N-linked (GlcNAc...)Sequence analysis1
Glycosylationi237N-linked (GlcNAc...)Sequence analysis1
Glycosylationi257N-linked (GlcNAc...)Sequence analysis1
Glycosylationi362N-linked (GlcNAc...)Sequence analysis1
Glycosylationi373N-linked (GlcNAc...)Sequence analysis1
Glycosylationi451N-linked (GlcNAc...)Sequence analysis1
Glycosylationi461N-linked (GlcNAc...)Sequence analysis1
Glycosylationi521N-linked (GlcNAc...)Sequence analysis1
Glycosylationi532N-linked (GlcNAc...)Sequence analysis1
Glycosylationi558N-linked (GlcNAc...)Sequence analysis1
Glycosylationi638N-linked (GlcNAc...)Sequence analysis1
Glycosylationi722N-linked (GlcNAc...)Sequence analysis1
Glycosylationi743N-linked (GlcNAc...)Sequence analysis1
Modified residuei848PhosphothreonineBy similarity1
Modified residuei856PhosphothreonineBy similarity1
Modified residuei932PhosphotyrosineBy similarity1
Modified residuei1022PhosphoserineBy similarity1
Modified residuei1030PhosphotyrosineBy similarity1
Modified residuei1141PhosphothreonineCombined sources1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9ZWC8.
PRIDEiQ9ZWC8.

PTM databases

iPTMnetiQ9ZWC8.

Expressioni

Tissue specificityi

Predominantly expressed in vascular tissues. From 7 day old seedlings, it is expressed in the columella cells of the root tip, in the vascular initials in the meristematic region of the root and in vascular tissues. After germination, it is expressed in the stele cell and in the early differentiation zone of the root, where the expression continues from the root to the hypocotyls and cotyledons following the midvein. In mature plants, it is expressed in the vasculature of the leaf, predominantly in the midvein, and in the vascular bundles of inflorescence stems. Localizes to procambial cells of the vascular bundles located between the differentiating xylem and the phloem.2 Publications

Gene expression databases

GenevisibleiQ9ZWC8. AT.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
BAK1Q94F625EBI-590903,EBI-617138
BKI1Q9FMZ04EBI-590903,EBI-1111615

Protein-protein interaction databases

BioGridi27235. 2 interactors.
IntActiQ9ZWC8. 3 interactors.
STRINGi3702.AT1G55610.1.

Structurei

Secondary structure

11166
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi33 – 44Combined sources12
Beta strandi46 – 48Combined sources3
Turni53 – 56Combined sources4
Helixi65 – 67Combined sources3
Beta strandi71 – 73Combined sources3
Beta strandi79 – 83Combined sources5
Beta strandi90 – 93Combined sources4
Helixi95 – 99Combined sources5
Beta strandi106 – 108Combined sources3
Beta strandi111 – 116Combined sources6
Beta strandi128 – 131Combined sources4
Beta strandi134 – 137Combined sources4
Helixi140 – 149Combined sources10
Beta strandi155 – 157Combined sources3
Beta strandi164 – 166Combined sources3
Beta strandi179 – 181Combined sources3
Beta strandi184 – 190Combined sources7
Turni193 – 198Combined sources6
Beta strandi205 – 207Combined sources3
Beta strandi214 – 216Combined sources3
Helixi217 – 219Combined sources3
Beta strandi230 – 232Combined sources3
Beta strandi235 – 239Combined sources5
Helixi247 – 249Combined sources3
Beta strandi255 – 257Combined sources3
Turni270 – 275Combined sources6
Beta strandi281 – 283Combined sources3
Beta strandi290 – 292Combined sources3
Helixi295 – 302Combined sources8
Beta strandi305 – 308Combined sources4
Helixi320 – 324Combined sources5
Beta strandi330 – 332Combined sources3
Beta strandi335 – 338Combined sources4
Helixi342 – 345Combined sources4
Helixi347 – 349Combined sources3
Beta strandi355 – 357Combined sources3
Beta strandi364 – 366Combined sources3
Helixi369 – 373Combined sources5
Beta strandi379 – 381Combined sources3
Beta strandi384 – 389Combined sources6
Beta strandi395 – 398Combined sources4
Beta strandi406 – 408Combined sources3
Helixi420 – 424Combined sources5
Beta strandi430 – 432Combined sources3
Beta strandi439 – 441Combined sources3
Helixi444 – 447Combined sources4
Beta strandi454 – 456Combined sources3
Beta strandi459 – 464Combined sources6
Turni468 – 472Combined sources5
Beta strandi478 – 481Combined sources4
Helixi493 – 497Combined sources5
Beta strandi503 – 505Combined sources3
Helixi517 – 521Combined sources5
Beta strandi527 – 529Combined sources3
Helixi541 – 545Combined sources5
Beta strandi551 – 553Combined sources3
Beta strandi556 – 559Combined sources4
Helixi565 – 568Combined sources4
Turni569 – 573Combined sources5
Turni578 – 581Combined sources4
Beta strandi583 – 590Combined sources8
Turni594 – 597Combined sources4
Beta strandi598 – 603Combined sources6
Helixi609 – 614Combined sources6
Turni616 – 620Combined sources5
Beta strandi623 – 630Combined sources8
Beta strandi636 – 639Combined sources4
Beta strandi642 – 645Combined sources4
Beta strandi652 – 654Combined sources3
Helixi657 – 661Combined sources5
Beta strandi667 – 669Combined sources3
Helixi681 – 685Combined sources5
Beta strandi690 – 693Combined sources4
Beta strandi696 – 701Combined sources6
Helixi705 – 709Combined sources5
Beta strandi715 – 717Combined sources3
Beta strandi720 – 723Combined sources4
Helixi733 – 735Combined sources3
Helixi738 – 741Combined sources4
Beta strandi745 – 749Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4J0MX-ray2.50A/B25-758[»]
ProteinModelPortaliQ9ZWC8.
SMRiQ9ZWC8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati78 – 99LRR 1Add BLAST22
Repeati103 – 124LRR 2Add BLAST22
Repeati126 – 147LRR 3Add BLAST22
Repeati152 – 173LRR 4Add BLAST22
Repeati176 – 197LRR 5Add BLAST22
Repeati202 – 224LRR 6Add BLAST23
Repeati227 – 248LRR 7Add BLAST22
Repeati252 – 274LRR 8Add BLAST23
Repeati278 – 300LRR 9Add BLAST23
Repeati303 – 325LRR 10Add BLAST23
Repeati327 – 349LRR 11Add BLAST23
Repeati352 – 375LRR 12Add BLAST24
Repeati376 – 397LRR 13Add BLAST22
Repeati403 – 424LRR 14Add BLAST22
Repeati427 – 449LRR 15Add BLAST23
Repeati451 – 473LRR 16Add BLAST23
Repeati476 – 498LRR 17Add BLAST23
Repeati500 – 522LRR 18Add BLAST23
Repeati524 – 547LRR 19Add BLAST24
Repeati548 – 570LRR 20Add BLAST23
Repeati664 – 686LRR 21Add BLAST23
Repeati688 – 710LRR 22Add BLAST23
Repeati712 – 734LRR 23Add BLAST23
Domaini859 – 1147Protein kinasePROSITE-ProRule annotationAdd BLAST289

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi66 – 73Cys pair 18
Motifi748 – 755Cys pair 28

Domaini

Contains two pairs of conservatively spaced Cys (Cys pair 1 and 2) possibly involved in forming some heterodimers.By similarity
A 70 amino acid island between the 19th and the 20th LRR is essential for the binding of brassinosteroids.By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 23 LRR (leucine-rich) repeats.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJWH. Eukaryota.
COG0515. LUCA.
COG4886. LUCA.
HOGENOMiHOG000116551.
InParanoidiQ9ZWC8.
OMAiCPATRIY.
OrthoDBiEOG093600TU.
PhylomeDBiQ9ZWC8.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
3.80.10.10. 4 hits.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR013210. LRR_N_plant-typ.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13516. LRR_6. 4 hits.
PF13855. LRR_8. 1 hit.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 8 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 4 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51450. LRR. 16 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ZWC8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQRWLLVLI LCFFTTSLVM GIHGKHLIND DFNETALLLA FKQNSVKSDP
60 70 80 90 100
NNVLGNWKYE SGRGSCSWRG VSCSDDGRIV GLDLRNSGLT GTLNLVNLTA
110 120 130 140 150
LPNLQNLYLQ GNYFSSGGDS SGSDCYLQVL DLSSNSISDY SMVDYVFSKC
160 170 180 190 200
SNLVSVNISN NKLVGKLGFA PSSLQSLTTV DLSYNILSDK IPESFISDFP
210 220 230 240 250
ASLKYLDLTH NNLSGDFSDL SFGICGNLTF FSLSQNNLSG DKFPITLPNC
260 270 280 290 300
KFLETLNISR NNLAGKIPNG EYWGSFQNLK QLSLAHNRLS GEIPPELSLL
310 320 330 340 350
CKTLVILDLS GNTFSGELPS QFTACVWLQN LNLGNNYLSG DFLNTVVSKI
360 370 380 390 400
TGITYLYVAY NNISGSVPIS LTNCSNLRVL DLSSNGFTGN VPSGFCSLQS
410 420 430 440 450
SPVLEKILIA NNYLSGTVPM ELGKCKSLKT IDLSFNELTG PIPKEIWMLP
460 470 480 490 500
NLSDLVMWAN NLTGTIPEGV CVKGGNLETL ILNNNLLTGS IPESISRCTN
510 520 530 540 550
MIWISLSSNR LTGKIPSGIG NLSKLAILQL GNNSLSGNVP RQLGNCKSLI
560 570 580 590 600
WLDLNSNNLT GDLPGELASQ AGLVMPGSVS GKQFAFVRNE GGTDCRGAGG
610 620 630 640 650
LVEFEGIRAE RLERLPMVHS CPATRIYSGM TMYTFSANGS MIYFDISYNA
660 670 680 690 700
VSGFIPPGYG NMGYLQVLNL GHNRITGTIP DSFGGLKAIG VLDLSHNNLQ
710 720 730 740 750
GYLPGSLGSL SFLSDLDVSN NNLTGPIPFG GQLTTFPVSR YANNSGLCGV
760 770 780 790 800
PLRPCGSAPR RPITSRIHAK KQTVATAVIA GIAFSFMCFV MLVMALYRVR
810 820 830 840 850
KVQKKEQKRE KYIESLPTSG SCSWKLSSVP EPLSINVATF EKPLRKLTFA
860 870 880 890 900
HLLEATNGFS AETMVGSGGF GEVYKAQLRD GSVVAIKKLI RITGQGDREF
910 920 930 940 950
MAEMETIGKI KHRNLVPLLG YCKVGEERLL VYEYMKWGSL ETVLHEKSSK
960 970 980 990 1000
KGGIYLNWAA RKKIAIGAAR GLAFLHHSCI PHIIHRDMKS SNVLLDEDFE
1010 1020 1030 1040 1050
ARVSDFGMAR LVSALDTHLS VSTLAGTPGY VPPEYYQSFR CTAKGDVYSY
1060 1070 1080 1090 1100
GVILLELLSG KKPIDPGEFG EDNNLVGWAK QLYREKRGAE ILDPELVTDK
1110 1120 1130 1140 1150
SGDVELFHYL KIASQCLDDR PFKRPTMIQL MAMFKEMKAD TEEDESLDEF
1160
SLKETPLVEE SRDKEP
Length:1,166
Mass (Da):127,424
Last modified:May 1, 1999 - v1
Checksum:i8C4DD9231A466AF7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ708660 mRNA. Translation: ACN59256.1.
AC002328 Genomic DNA. Translation: AAF79510.1.
CP002684 Genomic DNA. Translation: AEE33271.1.
CP002684 Genomic DNA. Translation: AEE33272.1.
PIRiF96598.
RefSeqiNP_001117501.1. NM_001124029.2.
NP_175957.1. NM_104437.3.
UniGeneiAt.66034.

Genome annotation databases

EnsemblPlantsiAT1G55610.1; AT1G55610.1; AT1G55610.
AT1G55610.2; AT1G55610.2; AT1G55610.
GeneIDi842010.
GrameneiAT1G55610.1; AT1G55610.1; AT1G55610.
AT1G55610.2; AT1G55610.2; AT1G55610.
KEGGiath:AT1G55610.

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ708660 mRNA. Translation: ACN59256.1.
AC002328 Genomic DNA. Translation: AAF79510.1.
CP002684 Genomic DNA. Translation: AEE33271.1.
CP002684 Genomic DNA. Translation: AEE33272.1.
PIRiF96598.
RefSeqiNP_001117501.1. NM_001124029.2.
NP_175957.1. NM_104437.3.
UniGeneiAt.66034.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4J0MX-ray2.50A/B25-758[»]
ProteinModelPortaliQ9ZWC8.
SMRiQ9ZWC8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27235. 2 interactors.
IntActiQ9ZWC8. 3 interactors.
STRINGi3702.AT1G55610.1.

PTM databases

iPTMnetiQ9ZWC8.

Proteomic databases

PaxDbiQ9ZWC8.
PRIDEiQ9ZWC8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G55610.1; AT1G55610.1; AT1G55610.
AT1G55610.2; AT1G55610.2; AT1G55610.
GeneIDi842010.
GrameneiAT1G55610.1; AT1G55610.1; AT1G55610.
AT1G55610.2; AT1G55610.2; AT1G55610.
KEGGiath:AT1G55610.

Organism-specific databases

TAIRiAT1G55610.

Phylogenomic databases

eggNOGiENOG410IJWH. Eukaryota.
COG0515. LUCA.
COG4886. LUCA.
HOGENOMiHOG000116551.
InParanoidiQ9ZWC8.
OMAiCPATRIY.
OrthoDBiEOG093600TU.
PhylomeDBiQ9ZWC8.

Miscellaneous databases

PROiQ9ZWC8.

Gene expression databases

GenevisibleiQ9ZWC8. AT.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
3.80.10.10. 4 hits.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR013210. LRR_N_plant-typ.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13516. LRR_6. 4 hits.
PF13855. LRR_8. 1 hit.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 8 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 4 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51450. LRR. 16 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBRL1_ARATH
AccessioniPrimary (citable) accession number: Q9ZWC8
Secondary accession number(s): C0LGH1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.