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Q9ZWB7

- PCS2_ARATH

UniProt

Q9ZWB7 - PCS2_ARATH

Protein

Glutathione gamma-glutamylcysteinyltransferase 2

Gene

PCS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Involved in the synthesis of phytochelatins (PC) and homophytochelatins (hPC), the heavy-metal-binding peptides of plants.3 Publications

    Catalytic activityi

    Glutathione + (Glu(-Cys))(n)-Gly = Gly + (Glu(-Cys))(n+1)-Gly.PROSITE-ProRule annotation

    Enzyme regulationi

    Requires cadmium for activity. Also activated in heterologous system by AsO43- ions, but not by Cu2+, Zn2+, Mn2+ or Ni2+ ions.1 Publication

    GO - Molecular functioni

    1. glutathione gamma-glutamylcysteinyltransferase activity Source: TAIR
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. phytochelatin biosynthetic process Source: TAIR
    2. response to metal ion Source: InterPro

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Ligandi

    Cadmium, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G03980-MONOMER.

    Protein family/group databases

    MEROPSiC83.A01.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione gamma-glutamylcysteinyltransferase 2 (EC:2.3.2.15)
    Alternative name(s):
    Phytochelatin synthase 2
    Short name:
    AtPCS2
    Gene namesi
    Name:PCS2
    Ordered Locus Names:At1g03980
    ORF Names:F21M11.9
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G03980.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 452452Glutathione gamma-glutamylcysteinyltransferase 2PRO_0000287211Add
    BLAST

    Proteomic databases

    PRIDEiQ9ZWB7.

    Expressioni

    Tissue specificityi

    Expressed in shoots, roots, leaves, stems and flowers.1 Publication

    Inductioni

    Not induced by cadmium or other heavy metal stress.

    Gene expression databases

    GenevestigatoriQ9ZWB7.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G03980.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ZWB7.
    SMRiQ9ZWB7. Positions 11-216.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 220220Peptidase C83PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili287 – 31529Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the phytochelatin synthase family.PROSITE-ProRule annotation
    Contains 1 peptidase C83 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG76926.
    HOGENOMiHOG000241441.
    InParanoidiQ9ZWB7.
    KOiK05941.
    OMAiCEPLEIV.
    PhylomeDBiQ9ZWB7.

    Family and domain databases

    InterProiIPR007719. Phytochelatin_synthase.
    IPR015407. Phytochelatin_synthase_C.
    [Graphical view]
    PfamiPF05023. Phytochelatin. 1 hit.
    PF09328. Phytochelatin_C. 2 hits.
    [Graphical view]
    PROSITEiPS51443. PCS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9ZWB7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSMASLYRRS LSPPAIDFAS FEGKQIFNEA LQKGTMEGFF GLISYFQTQS    50
    EPAFCGLASL SMVLNSLSID PGRKWKGPWR WFDESMLECC EPLEIVKDKG 100
    ISFGKVVCLA HSSGAKVEAF RTNQSTIDDF RKYVVKCSTS DNCHMISTYH 150
    RQVLKQTGTG HFSPIGGYNA ERDMALILDV ARFKYPPHWV PLKLLWDAMD 200
    SIDQSTGRRR GFMLISRPHR EPGLLYTLSC KDESWISIAK YLKEDVPRLV 250
    SSQHVDTIER ILYVVFKSLP ANFNQFIKWM AEIRRTEDVN QNLSSEEKSR 300
    LKLKQELLKQ VQETKLFKHV DKFLSSVYED NLPYVAAKVY CDGDEILSGY 350
    ESDESCCKET CVKCIKGLGE EKVTVVAYPS GNDVFTALLL ALPPQTWSGI 400
    KDQSLLQEMK QLISMVSHPT LLQQEVLHLR RQLEMLKRCQ ENKEDEELSA 450
    PA 452
    Length:452
    Mass (Da):51,551
    Last modified:May 1, 1999 - v1
    Checksum:i8E8FD573E9C00A76
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY044049 mRNA. Translation: AAK94671.1.
    AC003027 Genomic DNA. Translation: AAD10671.1.
    CP002684 Genomic DNA. Translation: AEE27641.1.
    BT029214 mRNA. Translation: ABJ17149.1.
    PIRiG86170.
    RefSeqiNP_171894.1. NM_100279.4.
    UniGeneiAt.26664.
    At.71149.

    Genome annotation databases

    EnsemblPlantsiAT1G03980.1; AT1G03980.1; AT1G03980.
    GeneIDi839354.
    KEGGiath:AT1G03980.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY044049 mRNA. Translation: AAK94671.1 .
    AC003027 Genomic DNA. Translation: AAD10671.1 .
    CP002684 Genomic DNA. Translation: AEE27641.1 .
    BT029214 mRNA. Translation: ABJ17149.1 .
    PIRi G86170.
    RefSeqi NP_171894.1. NM_100279.4.
    UniGenei At.26664.
    At.71149.

    3D structure databases

    ProteinModelPortali Q9ZWB7.
    SMRi Q9ZWB7. Positions 11-216.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT1G03980.1-P.

    Protein family/group databases

    MEROPSi C83.A01.

    Proteomic databases

    PRIDEi Q9ZWB7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G03980.1 ; AT1G03980.1 ; AT1G03980 .
    GeneIDi 839354.
    KEGGi ath:AT1G03980.

    Organism-specific databases

    TAIRi AT1G03980.

    Phylogenomic databases

    eggNOGi NOG76926.
    HOGENOMi HOG000241441.
    InParanoidi Q9ZWB7.
    KOi K05941.
    OMAi CEPLEIV.
    PhylomeDBi Q9ZWB7.

    Enzyme and pathway databases

    BioCyci ARA:AT1G03980-MONOMER.

    Gene expression databases

    Genevestigatori Q9ZWB7.

    Family and domain databases

    InterProi IPR007719. Phytochelatin_synthase.
    IPR015407. Phytochelatin_synthase_C.
    [Graphical view ]
    Pfami PF05023. Phytochelatin. 1 hit.
    PF09328. Phytochelatin_C. 2 hits.
    [Graphical view ]
    PROSITEi PS51443. PCS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Arabidopsis thaliana expresses a second functional phytochelatin synthase."
      Cazale A.-C., Clemens S.
      FEBS Lett. 507:215-219(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, LACK OF INDUCTION BY CADMIUM, TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Arabidopsis ORF clones."
      Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Expression of Arabidopsis phytochelatin synthase 2 is too low to complement an AtPCS1-defective Cad1-3 mutant."
      Lee S., Kang B.S.
      Mol. Cells 19:81-87(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Function of phytochelatin synthase in catabolism of glutathione-conjugates."
      Blum R., Beck A., Korte A., Stengel A., Letzel T., Lendzian K., Grill E.
      Plant J. 49:740-749(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPCS2_ARATH
    AccessioniPrimary (citable) accession number: Q9ZWB7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 15, 2007
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Expression of PCS2 is too low to complement a PCS1-defective mutant.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3