Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutathione gamma-glutamylcysteinyltransferase 2

Gene

PCS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in the synthesis of phytochelatins (PC) and homophytochelatins (hPC), the heavy-metal-binding peptides of plants.3 Publications

Miscellaneous

Expression of PCS2 is too low to complement a PCS1-defective mutant.

Catalytic activityi

Glutathione + (Glu(-Cys))(n)-Gly = Gly + (Glu(-Cys))(n+1)-Gly.PROSITE-ProRule annotation

Enzyme regulationi

Requires cadmium for activity. Also activated in heterologous system by AsO43- ions, but not by Cu2+, Zn2+, Mn2+ or Ni2+ ions.1 Publication

GO - Molecular functioni

  • glutathione gamma-glutamylcysteinyltransferase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • phytochelatin biosynthetic process Source: TAIR
  • response to metal ion Source: InterPro

Keywordsi

Molecular functionAcyltransferase, Transferase
LigandCadmium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT1G03980-MONOMER

Protein family/group databases

MEROPSiC83.004

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione gamma-glutamylcysteinyltransferase 2 (EC:2.3.2.15)
Alternative name(s):
Phytochelatin synthase 2
Short name:
AtPCS2
Gene namesi
Name:PCS2
Ordered Locus Names:At1g03980
ORF Names:F21M11.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G03980
TAIRilocus:2024172 AT1G03980

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002872111 – 452Glutathione gamma-glutamylcysteinyltransferase 2Add BLAST452

Proteomic databases

PaxDbiQ9ZWB7

PTM databases

iPTMnetiQ9ZWB7

Expressioni

Tissue specificityi

Expressed in shoots, roots, leaves, stems and flowers.1 Publication

Inductioni

Not induced by cadmium or other heavy metal stress.

Gene expression databases

ExpressionAtlasiQ9ZWB7 baseline and differential
GenevisibleiQ9ZWB7 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G03980.1

Structurei

3D structure databases

ProteinModelPortaliQ9ZWB7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 220Peptidase C83PROSITE-ProRule annotationAdd BLAST220

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili287 – 315Sequence analysisAdd BLAST29

Sequence similaritiesi

Belongs to the phytochelatin synthase family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0632 Eukaryota
ENOG4111H5N LUCA
HOGENOMiHOG000241441
InParanoidiQ9ZWB7
KOiK05941
OMAiECCEPLE
OrthoDBiEOG09360CHS
PhylomeDBiQ9ZWB7

Family and domain databases

Gene3Di3.90.70.30, 1 hit
InterProiView protein in InterPro
IPR007719 PCS_N
IPR038156 PCS_N_sf
IPR015407 Phytochelatin_synthase_C
PfamiView protein in Pfam
PF05023 Phytochelatin, 1 hit
PF09328 Phytochelatin_C, 2 hits
PROSITEiView protein in PROSITE
PS51443 PCS, 1 hit

Sequencei

Sequence statusi: Complete.

Q9ZWB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMASLYRRS LSPPAIDFAS FEGKQIFNEA LQKGTMEGFF GLISYFQTQS
60 70 80 90 100
EPAFCGLASL SMVLNSLSID PGRKWKGPWR WFDESMLECC EPLEIVKDKG
110 120 130 140 150
ISFGKVVCLA HSSGAKVEAF RTNQSTIDDF RKYVVKCSTS DNCHMISTYH
160 170 180 190 200
RQVLKQTGTG HFSPIGGYNA ERDMALILDV ARFKYPPHWV PLKLLWDAMD
210 220 230 240 250
SIDQSTGRRR GFMLISRPHR EPGLLYTLSC KDESWISIAK YLKEDVPRLV
260 270 280 290 300
SSQHVDTIER ILYVVFKSLP ANFNQFIKWM AEIRRTEDVN QNLSSEEKSR
310 320 330 340 350
LKLKQELLKQ VQETKLFKHV DKFLSSVYED NLPYVAAKVY CDGDEILSGY
360 370 380 390 400
ESDESCCKET CVKCIKGLGE EKVTVVAYPS GNDVFTALLL ALPPQTWSGI
410 420 430 440 450
KDQSLLQEMK QLISMVSHPT LLQQEVLHLR RQLEMLKRCQ ENKEDEELSA

PA
Length:452
Mass (Da):51,551
Last modified:May 1, 1999 - v1
Checksum:i8E8FD573E9C00A76
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY044049 mRNA Translation: AAK94671.1
AC003027 Genomic DNA Translation: AAD10671.1
CP002684 Genomic DNA Translation: ANM60703.1
BT029214 mRNA Translation: ABJ17149.1
PIRiG86170
RefSeqiNP_171894.1, NM_100279.5
UniGeneiAt.26664
At.71149

Genome annotation databases

EnsemblPlantsiAT1G03980.3; AT1G03980.3; AT1G03980
GeneIDi839354
GrameneiAT1G03980.3; AT1G03980.3; AT1G03980
KEGGiath:AT1G03980

Similar proteinsi

Entry informationi

Entry nameiPCS2_ARATH
AccessioniPrimary (citable) accession number: Q9ZWB7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 1, 1999
Last modified: April 25, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health