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Protein

12S seed storage protein CRD

Gene

CRD

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Seed storage protein.

GO - Molecular functioni

  • nutrient reservoir activity Source: UniProtKB

GO - Biological processi

  • seed maturation Source: UniProtKB

Keywordsi

Molecular functionSeed storage protein, Storage protein

Names & Taxonomyi

Protein namesi
Recommended name:
12S seed storage protein CRD
Alternative name(s):
Cruciferin D
Legumin-type globulin storage protein CRD
Cleaved into the following 2 chains:
Alternative name(s):
12S seed storage protein CRD acidic chain
Alternative name(s):
12S seed storage protein CRD basic chain
Gene namesi
Name:CRD
Synonyms:CRU2
Ordered Locus Names:At1g03890
ORF Names:F21M11.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G03890.
TAIRilocus:2024219. AT1G03890.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 252 PublicationsAdd BLAST25
ChainiPRO_000039992527 – 27012S seed storage protein CRD alpha chainAdd BLAST244
ChainiPRO_0000399926271 – 45112S seed storage protein CRD beta chainAdd BLAST181

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi36 ↔ 69By similarity
Modified residuei39Phosphoserine1 Publication1
Disulfide bondi112 ↔ 277Interchain (between alpha and beta chains)Sequence analysis
Modified residuei115PhosphothreonineBy similarity1
Modified residuei302PhosphoserineBy similarity1
Modified residuei396PhosphothreonineBy similarity1
Modified residuei437PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated.By similarity
Proteolytically processed during seed maturation at a conserved Asn-Gly peptide bond by an asparaginyl endopeptidase to produce two mature polypeptides referred to as alpha and beta subunits that are joined together by a disulfide bond.
Phosphorylated in seeds on some Tyr residues in response to abscisic acid (ABA).By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9ZWA9.

PTM databases

iPTMnetiQ9ZWA9.

Expressioni

Tissue specificityi

Accumulates in seeds 8 days after anthesis.2 Publications

Developmental stagei

First observed in siliques 15 days post anthesis and accumulates progressively as native isoforms or proteolytic fragments during the last week of seed maturation/desiccation. Present in dry seeds, but disappears during their germination (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiQ9ZWA9. baseline and differential.
GenevisibleiQ9ZWA9. AT.

Interactioni

Subunit structurei

Hexamer; each subunit is composed of an acidic and a basic chain derived from a single precursor and linked by a disulfide bond.

Protein-protein interaction databases

STRINGi3702.AT1G03890.1.

Structurei

3D structure databases

ProteinModelPortaliQ9ZWA9.
SMRiQ9ZWA9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHSE. Eukaryota.
ENOG410YATH. LUCA.
HOGENOMiHOG000217279.
InParanoidiQ9ZWA9.
OMAiETICAMR.
OrthoDBiEOG09360C8E.
PhylomeDBiQ9ZWA9.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiView protein in InterPro
IPR006044. 11S_seedstore_pln.
IPR006045. Cupin_1.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
PfamiView protein in Pfam
PF00190. Cupin_1. 2 hits.
PRINTSiPR00439. 11SGLOBULIN.
SMARTiView protein in SMART
SM00835. Cupin_1. 2 hits.
SUPFAMiSSF51182. SSF51182. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ZWA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHKLLFSLLS VVSLSFLLFF HGAEARQREA PFPNACHFSQ INSLAPAQAT
60 70 80 90 100
KFEAGQMEVW DHMSPELRCA GVTVARITLQ PNSIFLPAFF SPPALAYVVQ
110 120 130 140 150
GEGVMGTIAS GCPETFAEVE GSSGRGGGGD PGRRFEDMHQ KLENFRRGDV
160 170 180 190 200
FASLAGVSQW WYNRGDSDAV IVIVLDVTNR ENQLDQVPRM FQLAGSRTQE
210 220 230 240 250
EEQPLTWPSG NNAFSGFDPN IIAEAFKINI ETAKQLQNQK DNRGNIIRAN
260 270 280 290 300
GPLHFVIPPP REWQQDGIAN GIEETYCTAK IHENIDDPER SDHFSTRAGR
310 320 330 340 350
ISTLNSLNLP VLRLVRLNAL RGYLYSGGMV LPQWTANAHT VLYVTGGQAK
360 370 380 390 400
IQVVDDNGQS VFNEQVGQGQ IIVIPQGFAV SKTAGETGFE WISFKTNDNA
410 420 430 440 450
YINTLSGQTS YLRAVPVDVI KASYGVNEEE AKRIKFSQQE TMLSMTPSSS

S
Length:451
Mass (Da):49,675
Last modified:May 1, 1999 - v1
Checksum:i456BA2B5BE8BB9B0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti382K → R in BAD43747 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003027 Genomic DNA. Translation: AAD10679.1.
CP002684 Genomic DNA. Translation: AEE27629.1.
AY065432 mRNA. Translation: AAL38873.1.
AY117228 mRNA. Translation: AAM51303.1.
AK175984 mRNA. Translation: BAD43747.1.
PIRiF86169.
RefSeqiNP_171885.1. NM_100269.4.
UniGeneiAt.20175.

Genome annotation databases

EnsemblPlantsiAT1G03890.1; AT1G03890.1; AT1G03890.
GeneIDi839379.
GrameneiAT1G03890.1; AT1G03890.1; AT1G03890.
KEGGiath:AT1G03890.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCRU4_ARATH
AccessioniPrimary (citable) accession number: Q9ZWA9
Secondary accession number(s): Q67ZY3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: May 1, 1999
Last modified: July 5, 2017
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families