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Protein

Zinc finger protein MAGPIE

Gene

MGP

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that regulates tissue boundaries and asymmetric cell division (PubMed:17785527). Might be involved in the sequestration of 'SHORT-ROOT' to the nucleus (PubMed:17785527). Interacts with the SCR and MGP promoters (PubMed:21935722). Does not show transcription activity by itself, but regulates the transcription of downstream genes through interaction with other transcription factors (PubMed:21935722).2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri70 – 9223C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri111 – 14131C2H2-type 2CuratedAdd
BLAST
Zinc fingeri146 – 17025CCHC-type 1; atypicalCuratedAdd
BLAST
Zinc fingeri173 – 19624CCHC-type 2; atypicalCuratedAdd
BLAST

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • sequence-specific DNA binding transcription factor activity Source: TAIR
  • transcription regulatory region DNA binding Source: TAIR

GO - Biological processi

  • regulation of transcription, DNA-templated Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein MAGPIE1 Publication
Alternative name(s):
Protein indeterminate-domain 31 Publication
Gene namesi
Name:MGP1 Publication
Synonyms:IDD31 Publication
Ordered Locus Names:At1g03840Imported
ORF Names:F11M21.23Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G03840.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 506506Zinc finger protein MAGPIEPRO_0000337841Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei60 – 601PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9ZWA6.

Expressioni

Tissue specificityi

Expressed in the ground tissue and stele cells of embryos and 2-days post-germination roots but not in the quiescent center. Detected only in cells that perform asymmetric cell divisions.1 Publication

Inductioni

Up-regulated by the transcription factor JKD, and down-regulated by SHR, SCR and itself.1 Publication

Gene expression databases

ExpressionAtlasiQ9ZWA6. baseline and differential.

Interactioni

Subunit structurei

Interacts with SHR, SCR and JKD, but not with itself (PubMed:17785527). Interacts with SIEL (PubMed:21924907).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
JKDQ700D23EBI-1568600,EBI-1568562
SCRQ9M3843EBI-1568600,EBI-1250484

Protein-protein interaction databases

IntActiQ9ZWA6. 3 interactions.
STRINGi3702.AT1G03840.1.

Structurei

3D structure databases

ProteinModelPortaliQ9ZWA6.
SMRiQ9ZWA6. Positions 60-197.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi23 – 3210His-rich
Compositional biasi232 – 24918Pro-richAdd
BLAST
Compositional biasi251 – 2555Poly-His
Compositional biasi440 – 4434Poly-Asn
Compositional biasi480 – 4834Poly-Gly

Sequence similaritiesi

Contains 2 C2H2-type zinc fingers.PROSITE-ProRule annotationCurated
Contains 2 CCHC-type zinc fingers.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri70 – 9223C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri111 – 14131C2H2-type 2CuratedAdd
BLAST
Zinc fingeri146 – 17025CCHC-type 1; atypicalCuratedAdd
BLAST
Zinc fingeri173 – 19624CCHC-type 2; atypicalCuratedAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG246610.
HOGENOMiHOG000237608.
InParanoidiQ9ZWA6.
OMAiNQIVEDG.
PhylomeDBiQ9ZWA6.

Family and domain databases

Gene3Di3.30.160.60. 1 hit.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ZWA6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTEDQTISS SGGYVQSSST TDHVDHHHHD QHESLNPPLV KKKRNLPGNP
60 70 80 90 100
DPEAEVIALS PKTLMATNRF LCEICGKGFQ RDQNLQLHRR GHNLPWKLKQ
110 120 130 140 150
RTSKEVRKRV YVCPEKSCVH HHPTRALGDL TGIKKHFCRK HGEKKWKCEK
160 170 180 190 200
CAKRYAVQSD WKAHSKTCGT REYRCDCGTI FSRRDSFITH RAFCDALAEE
210 220 230 240 250
TARLNAASHL KSFAATAGSN LNYHYLMGTL IPSPSLPQPP SFPFGPPQPQ
260 270 280 290 300
HHHHHQFPIT TNNFDHQDVM KPASTLSLWS GGNINHHQQV TIEDRMAPQP
310 320 330 340 350
HSPQEDYNWV FGNANNHGEL ITTSDSLITH DNNINIVQSK ENANGATSLS
360 370 380 390 400
VPSLFSSVDQ ITQDANAASV AVANMSATAL LQKAAQMGAT SSTSPTTTIT
410 420 430 440 450
TDQSAYLQSF ASKSNQIVED GGSDRFFASF GSNSVELMSN NNNGLHEIGN
460 470 480 490 500
PRNGVTVVSG MGELQNYPWK RRRVDIGNAG GGGQTRDFLG VGVQTICHSS

SINGWI
Length:506
Mass (Da):55,826
Last modified:May 1, 1999 - v1
Checksum:iC27006B44C77D1C7
GO
Isoform 2 (identifier: Q9ZWA6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     50-51: Missing.

Note: May be due to a competing acceptor splice site.
Show »
Length:504
Mass (Da):55,614
Checksum:iE4757C11E865D056
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei50 – 512Missing in isoform 2. 1 PublicationVSP_036332

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY568648 mRNA. Translation: AAS79538.1.
AJ630476 mRNA. Translation: CAG25849.1.
AC003027 Genomic DNA. Translation: AAD10684.1.
CP002684 Genomic DNA. Translation: AEE27620.1.
CP002684 Genomic DNA. Translation: AEE27621.1.
BT006209 mRNA. Translation: AAP12858.1.
AK227837 mRNA. Translation: BAE99815.1.
PIRiA86169.
RefSeqiNP_001030951.1. NM_001035874.1. [Q9ZWA6-2]
NP_171880.1. NM_100263.3. [Q9ZWA6-1]
UniGeneiAt.42516.

Genome annotation databases

EnsemblPlantsiAT1G03840.1; AT1G03840.1; AT1G03840. [Q9ZWA6-1]
GeneIDi839396.
KEGGiath:AT1G03840.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY568648 mRNA. Translation: AAS79538.1.
AJ630476 mRNA. Translation: CAG25849.1.
AC003027 Genomic DNA. Translation: AAD10684.1.
CP002684 Genomic DNA. Translation: AEE27620.1.
CP002684 Genomic DNA. Translation: AEE27621.1.
BT006209 mRNA. Translation: AAP12858.1.
AK227837 mRNA. Translation: BAE99815.1.
PIRiA86169.
RefSeqiNP_001030951.1. NM_001035874.1. [Q9ZWA6-2]
NP_171880.1. NM_100263.3. [Q9ZWA6-1]
UniGeneiAt.42516.

3D structure databases

ProteinModelPortaliQ9ZWA6.
SMRiQ9ZWA6. Positions 60-197.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9ZWA6. 3 interactions.
STRINGi3702.AT1G03840.1.

Proteomic databases

PRIDEiQ9ZWA6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G03840.1; AT1G03840.1; AT1G03840. [Q9ZWA6-1]
GeneIDi839396.
KEGGiath:AT1G03840.

Organism-specific databases

TAIRiAT1G03840.

Phylogenomic databases

eggNOGiNOG246610.
HOGENOMiHOG000237608.
InParanoidiQ9ZWA6.
OMAiNQIVEDG.
PhylomeDBiQ9ZWA6.

Miscellaneous databases

PROiQ9ZWA6.

Gene expression databases

ExpressionAtlasiQ9ZWA6. baseline and differential.

Family and domain databases

Gene3Di3.30.160.60. 1 hit.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  6. "The maize INDETERMINATE1 flowering time regulator defines a highly conserved zinc finger protein family in higher plants."
    Colasanti J., Tremblay R., Wong A.Y., Coneva V., Kozaki A., Mable B.K.
    BMC Genomics 7:158-158(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  7. Cited for: INDUCTION BY SHR.
  8. "Arabidopsis JACKDAW and MAGPIE zinc finger proteins delimit asymmetric cell division and stabilize tissue boundaries by restricting SHORT-ROOT action."
    Welch D., Hassan H., Blilou I., Immink R., Heidstra R., Scheres B.
    Genes Dev. 21:2196-2204(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH SHR; SCR AND JKD.
  9. "Modulation of sugar metabolism by an INDETERMINATE DOMAIN transcription factor contributes to photoperiodic flowering in Arabidopsis."
    Seo P.J., Ryu J., Kang S.K., Park C.M.
    Plant J. 65:418-429(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  10. "An essential protein that interacts with endosomes and promotes movement of the SHORT-ROOT transcription factor."
    Koizumi K., Wu S., MacRae-Crerar A., Gallagher K.L.
    Curr. Biol. 21:1559-1564(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SIEL.
  11. "Activity of transcription factor JACKDAW is essential for SHR/SCR-dependent activation of SCARECROW and MAGPIE and is modulated by reciprocal interactions with MAGPIE, SCARECROW and SHORT ROOT."
    Ogasawara H., Kaimi R., Colasanti J., Kozaki A.
    Plant Mol. Biol. 77:489-499(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.

Entry informationi

Entry nameiIDD3_ARATH
AccessioniPrimary (citable) accession number: Q9ZWA6
Secondary accession number(s): Q700E8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: May 1, 1999
Last modified: June 24, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

MGP expression is SHR- and SCR-dependent.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.