Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9ZWA6

- MGP_ARATH

UniProt

Q9ZWA6 - MGP_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Zinc finger protein MAGPIE

Gene

MGP

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Probable transcription factor that regulates tissue boundaries and asymmetric cell division. Might be involved in the sequestration of 'SHORT-ROOT' to the nucleus.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri70 – 9223C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri146 – 16924C2H2-type 2; degeneratePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. sequence-specific DNA binding transcription factor activity Source: TAIR
  3. transcription regulatory region DNA binding Source: TAIR

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: TAIR
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein MAGPIE
Gene namesi
Name:MGP
Ordered Locus Names:At1g03840
ORF Names:F11M21.23
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G03840.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 506506Zinc finger protein MAGPIEPRO_0000337841Add
BLAST

Proteomic databases

PRIDEiQ9ZWA6.

Expressioni

Tissue specificityi

Expressed in the ground tissue and stele cells of embryos and 2-days post-germination roots but not in the quiescent center. Detected only in cells that perform asymmetric cell divisions.1 Publication

Gene expression databases

ExpressionAtlasiQ9ZWA6. baseline and differential.
GenevestigatoriQ9ZWA6.

Interactioni

Subunit structurei

Interacts with SHR, SCR and JKD, but not with itself.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
JKDQ700D23EBI-1568600,EBI-1568562
SCRQ9M3843EBI-1568600,EBI-1250484

Protein-protein interaction databases

IntActiQ9ZWA6. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9ZWA6.
SMRiQ9ZWA6. Positions 60-196.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi23 – 3210His-rich
Compositional biasi232 – 24918Pro-richAdd
BLAST
Compositional biasi251 – 2555Poly-His
Compositional biasi440 – 4434Poly-Asn
Compositional biasi480 – 4834Poly-Gly

Sequence similaritiesi

Contains 2 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri70 – 9223C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri146 – 16924C2H2-type 2; degeneratePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG246610.
HOGENOMiHOG000237608.
InParanoidiQ9ZWA6.
OMAiNQIVEDG.
PhylomeDBiQ9ZWA6.

Family and domain databases

Gene3Di3.30.160.60. 1 hit.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9ZWA6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTEDQTISS SGGYVQSSST TDHVDHHHHD QHESLNPPLV KKKRNLPGNP
60 70 80 90 100
DPEAEVIALS PKTLMATNRF LCEICGKGFQ RDQNLQLHRR GHNLPWKLKQ
110 120 130 140 150
RTSKEVRKRV YVCPEKSCVH HHPTRALGDL TGIKKHFCRK HGEKKWKCEK
160 170 180 190 200
CAKRYAVQSD WKAHSKTCGT REYRCDCGTI FSRRDSFITH RAFCDALAEE
210 220 230 240 250
TARLNAASHL KSFAATAGSN LNYHYLMGTL IPSPSLPQPP SFPFGPPQPQ
260 270 280 290 300
HHHHHQFPIT TNNFDHQDVM KPASTLSLWS GGNINHHQQV TIEDRMAPQP
310 320 330 340 350
HSPQEDYNWV FGNANNHGEL ITTSDSLITH DNNINIVQSK ENANGATSLS
360 370 380 390 400
VPSLFSSVDQ ITQDANAASV AVANMSATAL LQKAAQMGAT SSTSPTTTIT
410 420 430 440 450
TDQSAYLQSF ASKSNQIVED GGSDRFFASF GSNSVELMSN NNNGLHEIGN
460 470 480 490 500
PRNGVTVVSG MGELQNYPWK RRRVDIGNAG GGGQTRDFLG VGVQTICHSS

SINGWI
Length:506
Mass (Da):55,826
Last modified:May 1, 1999 - v1
Checksum:iC27006B44C77D1C7
GO
Isoform 2 (identifier: Q9ZWA6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     50-51: Missing.

Note: May be due to a competing acceptor splice site.

Show »
Length:504
Mass (Da):55,614
Checksum:iE4757C11E865D056
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei50 – 512Missing in isoform 2. 1 PublicationVSP_036332

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY568648 mRNA. Translation: AAS79538.1.
AJ630476 mRNA. Translation: CAG25849.1.
AC003027 Genomic DNA. Translation: AAD10684.1.
CP002684 Genomic DNA. Translation: AEE27620.1.
CP002684 Genomic DNA. Translation: AEE27621.1.
BT006209 mRNA. Translation: AAP12858.1.
AK227837 mRNA. Translation: BAE99815.1.
PIRiA86169.
RefSeqiNP_001030951.1. NM_001035874.1. [Q9ZWA6-2]
NP_171880.1. NM_100263.3. [Q9ZWA6-1]
UniGeneiAt.42516.

Genome annotation databases

EnsemblPlantsiAT1G03840.1; AT1G03840.1; AT1G03840. [Q9ZWA6-1]
GeneIDi839396.
KEGGiath:AT1G03840.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY568648 mRNA. Translation: AAS79538.1 .
AJ630476 mRNA. Translation: CAG25849.1 .
AC003027 Genomic DNA. Translation: AAD10684.1 .
CP002684 Genomic DNA. Translation: AEE27620.1 .
CP002684 Genomic DNA. Translation: AEE27621.1 .
BT006209 mRNA. Translation: AAP12858.1 .
AK227837 mRNA. Translation: BAE99815.1 .
PIRi A86169.
RefSeqi NP_001030951.1. NM_001035874.1. [Q9ZWA6-2 ]
NP_171880.1. NM_100263.3. [Q9ZWA6-1 ]
UniGenei At.42516.

3D structure databases

ProteinModelPortali Q9ZWA6.
SMRi Q9ZWA6. Positions 60-196.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9ZWA6. 3 interactions.

Proteomic databases

PRIDEi Q9ZWA6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G03840.1 ; AT1G03840.1 ; AT1G03840 . [Q9ZWA6-1 ]
GeneIDi 839396.
KEGGi ath:AT1G03840.

Organism-specific databases

TAIRi AT1G03840.

Phylogenomic databases

eggNOGi NOG246610.
HOGENOMi HOG000237608.
InParanoidi Q9ZWA6.
OMAi NQIVEDG.
PhylomeDBi Q9ZWA6.

Gene expression databases

ExpressionAtlasi Q9ZWA6. baseline and differential.
Genevestigatori Q9ZWA6.

Family and domain databases

Gene3Di 3.30.160.60. 1 hit.
InterProi IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
Pfami PF00096. zf-C2H2. 1 hit.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 2 hits.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  6. Cited for: INDUCTION BY SHR.
  7. "Arabidopsis JACKDAW and MAGPIE zinc finger proteins delimit asymmetric cell division and stabilize tissue boundaries by restricting SHORT-ROOT action."
    Welch D., Hassan H., Blilou I., Immink R., Heidstra R., Scheres B.
    Genes Dev. 21:2196-2204(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH SHR; SCR AND JKD.

Entry informationi

Entry nameiMGP_ARATH
AccessioniPrimary (citable) accession number: Q9ZWA6
Secondary accession number(s): Q700E8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: May 1, 1999
Last modified: October 29, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

MGP expression is SHR- and SCR-dependent.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3