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Q9ZWA6 (MGP_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger protein MAGPIE
Gene names
Name:MGP
Ordered Locus Names:At1g03840
ORF Names:F11M21.23
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length506 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable transcription factor that regulates tissue boundaries and asymmetric cell division. Might be involved in the sequestration of 'SHORT-ROOT' to the nucleus. Ref.7

Subunit structure

Interacts with SHR, SCR and JKD, but not with itself. Ref.7

Subcellular location

Nucleus Ref.7.

Tissue specificity

Expressed in the ground tissue and stele cells of embryos and 2-days post-germination roots but not in the quiescent center. Detected only in cells that perform asymmetric cell divisions. Ref.7

Miscellaneous

MGP expression is SHR- and SCR-dependent.

Sequence similarities

Contains 2 C2H2-type zinc fingers.

Binary interactions

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9ZWA6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9ZWA6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     50-51: Missing.
Note: May be due to a competing acceptor splice site.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 506506Zinc finger protein MAGPIE
PRO_0000337841

Regions

Zinc finger70 – 9223C2H2-type 1
Zinc finger146 – 16924C2H2-type 2; degenerate
Compositional bias23 – 3210His-rich
Compositional bias232 – 24918Pro-rich
Compositional bias251 – 2555Poly-His
Compositional bias440 – 4434Poly-Asn
Compositional bias480 – 4834Poly-Gly

Natural variations

Alternative sequence50 – 512Missing in isoform 2.
VSP_036332

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: C27006B44C77D1C7

FASTA50655,826
        10         20         30         40         50         60 
MTTEDQTISS SGGYVQSSST TDHVDHHHHD QHESLNPPLV KKKRNLPGNP DPEAEVIALS 

        70         80         90        100        110        120 
PKTLMATNRF LCEICGKGFQ RDQNLQLHRR GHNLPWKLKQ RTSKEVRKRV YVCPEKSCVH 

       130        140        150        160        170        180 
HHPTRALGDL TGIKKHFCRK HGEKKWKCEK CAKRYAVQSD WKAHSKTCGT REYRCDCGTI 

       190        200        210        220        230        240 
FSRRDSFITH RAFCDALAEE TARLNAASHL KSFAATAGSN LNYHYLMGTL IPSPSLPQPP 

       250        260        270        280        290        300 
SFPFGPPQPQ HHHHHQFPIT TNNFDHQDVM KPASTLSLWS GGNINHHQQV TIEDRMAPQP 

       310        320        330        340        350        360 
HSPQEDYNWV FGNANNHGEL ITTSDSLITH DNNINIVQSK ENANGATSLS VPSLFSSVDQ 

       370        380        390        400        410        420 
ITQDANAASV AVANMSATAL LQKAAQMGAT SSTSPTTTIT TDQSAYLQSF ASKSNQIVED 

       430        440        450        460        470        480 
GGSDRFFASF GSNSVELMSN NNNGLHEIGN PRNGVTVVSG MGELQNYPWK RRRVDIGNAG 

       490        500 
GGGQTRDFLG VGVQTICHSS SINGWI 

« Hide

Isoform 2 [UniParc].

Checksum: E4757C11E865D056
Show »

FASTA50455,614

References

« Hide 'large scale' references
[1]"Genome-wide ORFeome cloning and analysis of Arabidopsis transcription factor genes."
Gong W., Shen Y.-P., Ma L.-G., Pan Y., Du Y.-L., Wang D.-H., Yang J.-Y., Hu L.-D., Liu X.-F., Dong C.-X., Ma L., Chen Y.-H., Yang X.-Y., Gao Y., Zhu D., Tan X., Mu J.-Y., Zhang D.-B. expand/collapse author list , Liu Y.-L., Dinesh-Kumar S.P., Li Y., Wang X.-P., Gu H.-Y., Qu L.-J., Bai S.-N., Lu Y.-T., Li J.-Y., Zhao J.-D., Zuo J., Huang H., Deng X.-W., Zhu Y.-X.
Plant Physiol. 135:773-782(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[6]"Whole-genome analysis of the SHORT-ROOT developmental pathway in Arabidopsis."
Levesque M.P., Vernoux T., Busch W., Cui H., Wang J.Y., Blilou I., Hassan H., Nakajima K., Matsumoto N., Lohmann J.U., Scheres B., Benfey P.N.
PLoS Biol. 4:739-752(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY SHR.
[7]"Arabidopsis JACKDAW and MAGPIE zinc finger proteins delimit asymmetric cell division and stabilize tissue boundaries by restricting SHORT-ROOT action."
Welch D., Hassan H., Blilou I., Immink R., Heidstra R., Scheres B.
Genes Dev. 21:2196-2204(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH SHR; SCR AND JKD.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY568648 mRNA. Translation: AAS79538.1.
AJ630476 mRNA. Translation: CAG25849.1.
AC003027 Genomic DNA. Translation: AAD10684.1.
CP002684 Genomic DNA. Translation: AEE27620.1.
CP002684 Genomic DNA. Translation: AEE27621.1.
BT006209 mRNA. Translation: AAP12858.1.
AK227837 mRNA. Translation: BAE99815.1.
PIRA86169.
RefSeqNP_001030951.1. NM_001035874.1. [Q9ZWA6-2]
NP_171880.1. NM_100263.3. [Q9ZWA6-1]
UniGeneAt.42516.

3D structure databases

ProteinModelPortalQ9ZWA6.
SMRQ9ZWA6. Positions 60-196.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9ZWA6. 3 interactions.

Proteomic databases

PRIDEQ9ZWA6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G03840.1; AT1G03840.1; AT1G03840. [Q9ZWA6-1]
GeneID839396.
KEGGath:AT1G03840.

Organism-specific databases

TAIRAT1G03840.

Phylogenomic databases

eggNOGNOG246610.
HOGENOMHOG000237608.
InParanoidQ9ZWA6.
OMANQIVEDG.
PhylomeDBQ9ZWA6.

Gene expression databases

ArrayExpressQ9ZWA6.
GenevestigatorQ9ZWA6.

Family and domain databases

Gene3D3.30.160.60. 1 hit.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMGP_ARATH
AccessionPrimary (citable) accession number: Q9ZWA6
Secondary accession number(s): Q700E8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: May 1, 1999
Last modified: June 11, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names