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Protein

Zinc finger protein MAGPIE

Gene

MGP

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that regulates tissue boundaries and asymmetric cell division (PubMed:17785527). Might be involved in the sequestration of 'SHORT-ROOT' to the nucleus (PubMed:17785527). Interacts with the SCR and MGP promoters (PubMed:21935722). Does not show transcription activity by itself, but regulates the transcription of downstream genes through interaction with other transcription factors (PubMed:21935722).2 Publications

Miscellaneous

MGP expression is SHR- and SCR-dependent.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri70 – 92C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri111 – 141C2H2-type 2CuratedAdd BLAST31
Zinc fingeri146 – 170CCHC-type 1; atypicalCuratedAdd BLAST25
Zinc fingeri173 – 196CCHC-type 2; atypicalCuratedAdd BLAST24

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: TAIR
  • transcription regulatory region DNA binding Source: TAIR

GO - Biological processi

  • asymmetric cell division Source: TAIR
  • regulation of transcription, DNA-templated Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein MAGPIE1 Publication
Alternative name(s):
Protein indeterminate-domain 31 Publication
Gene namesi
Name:MGP1 Publication
Synonyms:IDD31 Publication
Ordered Locus Names:At1g03840Imported
ORF Names:F11M21.23Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G03840.
TAIRilocus:2024193. AT1G03840.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003378411 – 506Zinc finger protein MAGPIEAdd BLAST506

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei60PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9ZWA6.
PRIDEiQ9ZWA6.

PTM databases

iPTMnetiQ9ZWA6.

Expressioni

Tissue specificityi

Expressed in the ground tissue and stele cells of embryos and 2-days post-germination roots but not in the quiescent center. Detected only in cells that perform asymmetric cell divisions.1 Publication

Inductioni

Up-regulated by the transcription factor JKD, and down-regulated by SHR, SCR and itself.1 Publication

Gene expression databases

ExpressionAtlasiQ9ZWA6. baseline and differential.
GenevisibleiQ9ZWA6. AT.

Interactioni

Subunit structurei

Interacts with SHR, SCR and JKD, but not with itself (PubMed:17785527). Interacts with SIEL (PubMed:21924907).2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi24631. 2 interactors.
IntActiQ9ZWA6. 7 interactors.
STRINGi3702.AT1G03840.1.

Structurei

3D structure databases

ProteinModelPortaliQ9ZWA6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi23 – 32His-rich10
Compositional biasi232 – 249Pro-richAdd BLAST18
Compositional biasi251 – 255Poly-His5
Compositional biasi440 – 443Poly-Asn4
Compositional biasi480 – 483Poly-Gly4

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri70 – 92C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri111 – 141C2H2-type 2CuratedAdd BLAST31
Zinc fingeri146 – 170CCHC-type 1; atypicalCuratedAdd BLAST25
Zinc fingeri173 – 196CCHC-type 2; atypicalCuratedAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
HOGENOMiHOG000237608.
InParanoidiQ9ZWA6.
OMAiNGLHEIG.
OrthoDBiEOG09360BHF.
PhylomeDBiQ9ZWA6.

Family and domain databases

InterProiView protein in InterPro
IPR036236. Znf_C2H2_sf.
IPR013087. Znf_C2H2_type.
SUPFAMiSSF57667. SSF57667. 2 hits.
PROSITEiView protein in PROSITE
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ZWA6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTEDQTISS SGGYVQSSST TDHVDHHHHD QHESLNPPLV KKKRNLPGNP
60 70 80 90 100
DPEAEVIALS PKTLMATNRF LCEICGKGFQ RDQNLQLHRR GHNLPWKLKQ
110 120 130 140 150
RTSKEVRKRV YVCPEKSCVH HHPTRALGDL TGIKKHFCRK HGEKKWKCEK
160 170 180 190 200
CAKRYAVQSD WKAHSKTCGT REYRCDCGTI FSRRDSFITH RAFCDALAEE
210 220 230 240 250
TARLNAASHL KSFAATAGSN LNYHYLMGTL IPSPSLPQPP SFPFGPPQPQ
260 270 280 290 300
HHHHHQFPIT TNNFDHQDVM KPASTLSLWS GGNINHHQQV TIEDRMAPQP
310 320 330 340 350
HSPQEDYNWV FGNANNHGEL ITTSDSLITH DNNINIVQSK ENANGATSLS
360 370 380 390 400
VPSLFSSVDQ ITQDANAASV AVANMSATAL LQKAAQMGAT SSTSPTTTIT
410 420 430 440 450
TDQSAYLQSF ASKSNQIVED GGSDRFFASF GSNSVELMSN NNNGLHEIGN
460 470 480 490 500
PRNGVTVVSG MGELQNYPWK RRRVDIGNAG GGGQTRDFLG VGVQTICHSS

SINGWI
Length:506
Mass (Da):55,826
Last modified:May 1, 1999 - v1
Checksum:iC27006B44C77D1C7
GO
Isoform 2 (identifier: Q9ZWA6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     50-51: Missing.

Note: May be due to a competing acceptor splice site.
Show »
Length:504
Mass (Da):55,614
Checksum:iE4757C11E865D056
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03633250 – 51Missing in isoform 2. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY568648 mRNA. Translation: AAS79538.1.
AJ630476 mRNA. Translation: CAG25849.1.
AC003027 Genomic DNA. Translation: AAD10684.1.
CP002684 Genomic DNA. Translation: AEE27620.1.
CP002684 Genomic DNA. Translation: AEE27621.1.
BT006209 mRNA. Translation: AAP12858.1.
AK227837 mRNA. Translation: BAE99815.1.
PIRiA86169.
RefSeqiNP_001030951.1. NM_001035874.1. [Q9ZWA6-2]
NP_171880.1. NM_100263.4. [Q9ZWA6-1]
UniGeneiAt.42516.

Genome annotation databases

EnsemblPlantsiAT1G03840.1; AT1G03840.1; AT1G03840. [Q9ZWA6-1]
AT1G03840.2; AT1G03840.2; AT1G03840. [Q9ZWA6-2]
GeneIDi839396.
GrameneiAT1G03840.1; AT1G03840.1; AT1G03840.
AT1G03840.2; AT1G03840.2; AT1G03840.
KEGGiath:AT1G03840.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiIDD3_ARATH
AccessioniPrimary (citable) accession number: Q9ZWA6
Secondary accession number(s): Q700E8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: May 1, 1999
Last modified: November 22, 2017
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names