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Protein

Cytokinin hydroxylase

Gene

CYP735A2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytokinin hydroxylase that catalyzes the biosynthesis of trans-zeatin via the isopentenyladenine riboside 5'-monophosphate (iPRMP)-dependent pathway. Can use isopentenyladenosine-5'-monophosphate, isopentenyladenosine-5'-diphosphate and isopentenyladenosine-5'-triphosphate as substrate.1 Publication

Catalytic activityi

Isopentenyladenosine-5'-monophosphate + NADPH + O2 = trans-zeatin riboside monophosphate + NADP+ + H2O.
Isopentenyladenosine-5'-diphosphate + NADPH + O2 = trans-zeatin riboside diphosphate + NADP+ + H2O.
Isopentenyladenosine-5'-triphosphate + NADPH + O2 = trans-zeatin riboside triphosphate + NADP+ + H2O.

Cofactori

hemeBy similarity

Kineticsi

  1. KM=0.39 µM for isopentenyladenine riboside 5'-monophosphate
  2. KM=0.76 µM for isopentenyladenine riboside 5'-diphosphate
  3. KM=4.9 µM for isopentenyladenine riboside 5'-triphosphate

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi458Iron (heme axial ligand)By similarity1

    GO - Molecular functioni

    GO - Biological processi

    • trans-zeatin biosynthetic process Source: UniProtKB

    Keywordsi

    Molecular functionMonooxygenase, Oxidoreductase
    LigandHeme, Iron, Metal-binding, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytokinin hydroxylase (EC:1.14.13.-)
    Alternative name(s):
    Cytochrome P450 35A2
    Gene namesi
    Name:CYP735A2
    Ordered Locus Names:At1g67110
    ORF Names:F5A8.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    AraportiAT1G67110
    TAIRilocus:2033656 AT1G67110

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei2 – 22HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004112091 – 512Cytokinin hydroxylaseAdd BLAST512

    Proteomic databases

    PaxDbiQ9ZW95

    Expressioni

    Tissue specificityi

    Specifically expressed in roots.1 Publication

    Inductioni

    By trans-zeatin, cis-zeatin and isopentenyladenine in roots. Down-regulated by auxin and abscisic acid in roots.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9ZW95 baseline and differential
    GenevisibleiQ9ZW95 AT

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G67110.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ZW95
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG0157 Eukaryota
    COG2124 LUCA
    HOGENOMiHOG000039125
    InParanoidiQ9ZW95
    KOiK10717
    OMAiMAFEDIQ
    OrthoDBiEOG093607JN
    PhylomeDBiQ9ZW95

    Family and domain databases

    Gene3Di1.10.630.10, 1 hit
    InterProiView protein in InterPro
    IPR001128 Cyt_P450
    IPR002401 Cyt_P450_E_grp-I
    IPR036396 Cyt_P450_sf
    PfamiView protein in Pfam
    PF00067 p450, 1 hit
    PRINTSiPR00463 EP450I
    PR00385 P450
    SUPFAMiSSF48264 SSF48264, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9ZW95-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MMVTLVLKYV LVIVMTLILR VLYDSICCYF LTPRRIKKFM ERQGITGPKP
    60 70 80 90 100
    RLLTGNIIDI SKMLSHSASN DCSSIHHNIV PRLLPHYVSW SKQYGKRFIM
    110 120 130 140 150
    WNGTEPRLCL TETEMIKELL TKHNPVTGKS WLQQQGTKGF IGRGLLMANG
    160 170 180 190 200
    EAWHHQRHMA APAFTRDRLK GYAKHMVECT KMMAERLRKE VGEEVEIGEE
    210 220 230 240 250
    MRRLTADIIS RTEFGSSCDK GKELFSLLTV LQRLCAQATR HLCFPGSRFL
    260 270 280 290 300
    PSKYNREIKS LKTEVERLLM EIIDSRKDSV EIGRSSSYGD DLLGLLLNQM
    310 320 330 340 350
    DSNKNNLNVQ MIMDECKTFF FTGHETTSLL LTWTLMLLAH NPTWQDNVRD
    360 370 380 390 400
    EVRQVCGQDG VPSVEQLSSL TSLNKVINES LRLYPPATLL PRMAFEDIKL
    410 420 430 440 450
    GDLIIPKGLS IWIPVLAIHH SNELWGEDAN EFNPERFTTR SFASSRHFMP
    460 470 480 490 500
    FAAGPRNCIG QTFAMMEAKI ILAMLVSKFS FAISENYRHA PIVVLTIKPK
    510
    YGVQLVLKPL DL
    Length:512
    Mass (Da):58,463
    Last modified:May 1, 1999 - v1
    Checksum:i038844E878935BC7
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC004146 Genomic DNA Translation: AAD10659.1
    CP002684 Genomic DNA Translation: AEE34597.1
    BT011622 mRNA Translation: AAS47628.1
    BT012626 mRNA Translation: AAT06445.1
    PIRiA96695
    RefSeqiNP_176882.1, NM_105381.5
    UniGeneiAt.35690

    Genome annotation databases

    EnsemblPlantsiAT1G67110.1; AT1G67110.1; AT1G67110
    GeneIDi843031
    GrameneiAT1G67110.1; AT1G67110.1; AT1G67110
    KEGGiath:AT1G67110

    Similar proteinsi

    Entry informationi

    Entry nameiC7352_ARATH
    AccessioniPrimary (citable) accession number: Q9ZW95
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 27, 2011
    Last sequence update: May 1, 1999
    Last modified: April 25, 2018
    This is version 125 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health