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Protein

Proliferating cell nuclear antigen 2

Gene

PCNA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand (By similarity). May be involved in UV resistance.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi61 – 8020Sequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-ATH-110312. Translesion synthesis by REV1.
R-ATH-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-ATH-110320. Translesion Synthesis by POLH.
R-ATH-113510. E2F mediated regulation of DNA replication.
R-ATH-174411. Polymerase switching on the C-strand of the telomere.
R-ATH-174414. Processive synthesis on the C-strand of the telomere.
R-ATH-174417. Telomere C-strand (Lagging Strand) Synthesis.
R-ATH-174437. Removal of the Flap Intermediate from the C-strand.
R-ATH-4615885. SUMOylation of DNA replication proteins.
R-ATH-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
R-ATH-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-ATH-5655862. Translesion synthesis by POLK.
R-ATH-5656121. Translesion synthesis by POLI.
R-ATH-5656169. Termination of translesion DNA synthesis.
R-ATH-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-ATH-5696400. Dual Incision in GG-NER.
R-ATH-6782135. Dual incision in TC-NER.
R-ATH-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-ATH-69091. Polymerase switching.
R-ATH-69109. Leading Strand Synthesis.
R-ATH-69166. Removal of the Flap Intermediate.
R-ATH-69183. Processive synthesis on the lagging strand.
R-ATH-69205. G1/S-Specific Transcription.

Names & Taxonomyi

Protein namesi
Recommended name:
Proliferating cell nuclear antigen 2
Short name:
PCNA 2
Gene namesi
Name:PCNA2
Ordered Locus Names:At2g29570
ORF Names:F16P2.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G29570.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 264264Proliferating cell nuclear antigen 2PRO_0000149178Add
BLAST

Proteomic databases

PaxDbiQ9ZW35.
PRIDEiQ9ZW35.

Expressioni

Gene expression databases

GenevisibleiQ9ZW35. AT.

Interactioni

Subunit structurei

Homo- and heterotrimer. Interacts with POLH, ATXR5 and ATXR6.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
POLHQ8H2D52EBI-1810473,EBI-1810451

Protein-protein interaction databases

BioGridi2856. 7 interactions.
IntActiQ9ZW35. 3 interactions.
STRINGi3702.AT2G29570.1.

Structurei

Secondary structure

1
264
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 76Combined sources
Helixi9 – 2214Combined sources
Beta strandi24 – 307Combined sources
Beta strandi32 – 409Combined sources
Beta strandi44 – 5310Combined sources
Helixi54 – 563Combined sources
Beta strandi57 – 626Combined sources
Beta strandi66 – 716Combined sources
Helixi72 – 809Combined sources
Beta strandi87 – 926Combined sources
Beta strandi97 – 1048Combined sources
Beta strandi108 – 11710Combined sources
Beta strandi134 – 1407Combined sources
Helixi141 – 15111Combined sources
Turni152 – 1543Combined sources
Beta strandi156 – 1638Combined sources
Beta strandi166 – 1738Combined sources
Beta strandi176 – 1827Combined sources
Helixi191 – 1933Combined sources
Beta strandi196 – 2016Combined sources
Beta strandi203 – 2086Combined sources
Helixi209 – 2157Combined sources
Helixi216 – 2216Combined sources
Beta strandi223 – 2297Combined sources
Beta strandi235 – 2417Combined sources
Turni242 – 2443Combined sources
Beta strandi245 – 2517Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZVWX-ray2.50A/B/C/D/E/F/G/H1-255[»]
ProteinModelPortaliQ9ZW35.
SMRiQ9ZW35. Positions 1-255.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9ZW35.

Family & Domainsi

Sequence similaritiesi

Belongs to the PCNA family.Curated

Phylogenomic databases

eggNOGiKOG1636. Eukaryota.
COG0592. LUCA.
HOGENOMiHOG000211098.
InParanoidiQ9ZW35.
KOiK04802.
OMAiSDGFDKY.
PhylomeDBiQ9ZW35.

Family and domain databases

HAMAPiMF_00317. DNApol_clamp_arch.
InterProiIPR000730. Pr_cel_nuc_antig.
IPR022649. Pr_cel_nuc_antig_C.
IPR022659. Pr_cel_nuc_antig_CS.
IPR022648. Pr_cel_nuc_antig_N.
[Graphical view]
PANTHERiPTHR11352. PTHR11352. 1 hit.
PfamiPF02747. PCNA_C. 1 hit.
PF00705. PCNA_N. 1 hit.
[Graphical view]
PRINTSiPR00339. PCNACYCLIN.
TIGRFAMsiTIGR00590. pcna. 1 hit.
PROSITEiPS01251. PCNA_1. 1 hit.
PS00293. PCNA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZW35-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLELRLVQGS LLKKVLEAVK DLVNDANFDC STTGFSLQAM DSSHVALVSL
60 70 80 90 100
LLRSEGFEHY RCDRNLSMGM NLGNMSKMLK CAGNDDIITI KADDGSDTVT
110 120 130 140 150
FMFESPTQDK IADFEMKLMD IDSEHLGIPD AEYHSIVRMP SGEFSRICKD
160 170 180 190 200
LSSIGDTVVI SVTKEGVKFS TAGDIGTANI VLRQNTTVDK PEDAIVIEMN
210 220 230 240 250
EPVSLSFALR YMNSFTKATP LSETVTISLS SELPVVVEYK VAEMGYIRYY
260
LAPKIEEEED TKPE
Length:264
Mass (Da):29,222
Last modified:May 1, 1999 - v1
Checksum:i5A10FF87ABC86EE9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU072918 mRNA. Translation: ABU25234.1.
AC004561 Genomic DNA. Translation: AAC95182.1.
CP002685 Genomic DNA. Translation: AEC08274.1.
BT029466 mRNA. Translation: ABK59695.1.
PIRiH84697.
RefSeqiNP_180517.1. NM_128510.2.
UniGeneiAt.23904.
At.64384.

Genome annotation databases

EnsemblPlantsiAT2G29570.1; AT2G29570.1; AT2G29570.
GeneIDi817506.
GrameneiAT2G29570.1; AT2G29570.1; AT2G29570.
KEGGiath:AT2G29570.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU072918 mRNA. Translation: ABU25234.1.
AC004561 Genomic DNA. Translation: AAC95182.1.
CP002685 Genomic DNA. Translation: AEC08274.1.
BT029466 mRNA. Translation: ABK59695.1.
PIRiH84697.
RefSeqiNP_180517.1. NM_128510.2.
UniGeneiAt.23904.
At.64384.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZVWX-ray2.50A/B/C/D/E/F/G/H1-255[»]
ProteinModelPortaliQ9ZW35.
SMRiQ9ZW35. Positions 1-255.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2856. 7 interactions.
IntActiQ9ZW35. 3 interactions.
STRINGi3702.AT2G29570.1.

Proteomic databases

PaxDbiQ9ZW35.
PRIDEiQ9ZW35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G29570.1; AT2G29570.1; AT2G29570.
GeneIDi817506.
GrameneiAT2G29570.1; AT2G29570.1; AT2G29570.
KEGGiath:AT2G29570.

Organism-specific databases

TAIRiAT2G29570.

Phylogenomic databases

eggNOGiKOG1636. Eukaryota.
COG0592. LUCA.
HOGENOMiHOG000211098.
InParanoidiQ9ZW35.
KOiK04802.
OMAiSDGFDKY.
PhylomeDBiQ9ZW35.

Enzyme and pathway databases

ReactomeiR-ATH-110312. Translesion synthesis by REV1.
R-ATH-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-ATH-110320. Translesion Synthesis by POLH.
R-ATH-113510. E2F mediated regulation of DNA replication.
R-ATH-174411. Polymerase switching on the C-strand of the telomere.
R-ATH-174414. Processive synthesis on the C-strand of the telomere.
R-ATH-174417. Telomere C-strand (Lagging Strand) Synthesis.
R-ATH-174437. Removal of the Flap Intermediate from the C-strand.
R-ATH-4615885. SUMOylation of DNA replication proteins.
R-ATH-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
R-ATH-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-ATH-5655862. Translesion synthesis by POLK.
R-ATH-5656121. Translesion synthesis by POLI.
R-ATH-5656169. Termination of translesion DNA synthesis.
R-ATH-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-ATH-5696400. Dual Incision in GG-NER.
R-ATH-6782135. Dual incision in TC-NER.
R-ATH-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-ATH-69091. Polymerase switching.
R-ATH-69109. Leading Strand Synthesis.
R-ATH-69166. Removal of the Flap Intermediate.
R-ATH-69183. Processive synthesis on the lagging strand.
R-ATH-69205. G1/S-Specific Transcription.

Miscellaneous databases

EvolutionaryTraceiQ9ZW35.
PROiQ9ZW35.

Gene expression databases

GenevisibleiQ9ZW35. AT.

Family and domain databases

HAMAPiMF_00317. DNApol_clamp_arch.
InterProiIPR000730. Pr_cel_nuc_antig.
IPR022649. Pr_cel_nuc_antig_C.
IPR022659. Pr_cel_nuc_antig_CS.
IPR022648. Pr_cel_nuc_antig_N.
[Graphical view]
PANTHERiPTHR11352. PTHR11352. 1 hit.
PfamiPF02747. PCNA_C. 1 hit.
PF00705. PCNA_N. 1 hit.
[Graphical view]
PRINTSiPR00339. PCNACYCLIN.
TIGRFAMsiTIGR00590. pcna. 1 hit.
PROSITEiPS01251. PCNA_1. 1 hit.
PS00293. PCNA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Di Rubbo A., Vonarx E.J., Kunz B.A.
    Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.
    Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Two cell-cycle regulated SET-domain proteins interact with proliferating cell nuclear antigen (PCNA) in Arabidopsis."
    Raynaud C., Sozzani R., Glab N., Domenichini S., Perennes C., Cella R., Kondorosi E., Bergounioux C.
    Plant J. 47:395-407(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ATXR5 AND ATXR6.
  6. "Arabidopsis thaliana Y-family DNA polymerase eta catalyses translesion synthesis and interacts functionally with PCNA2."
    Anderson H.J., Vonarx E.J., Pastushok L., Nakagawa M., Katafuchi A., Gruz P., Di Rubbo A., Grice D.M., Osmond M.J., Sakamoto A.N., Nohmi T., Xiao W., Kunz B.A.
    Plant J. 55:895-908(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN UV RESISTANCE, INTERACTION WITH POLH.
  7. "Crystal structures of the Arabidopsis thaliana proliferating cell nuclear antigen 1 and 2 proteins complexed with the human p21 C-terminal segment."
    Strzalka W., Oyama T., Tori K., Morikawa K.
    Protein Sci. 18:1072-1080(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 1-256 IN COMPLEX WITH HUMAN P21 PEPTIDE, SUBUNIT.

Entry informationi

Entry nameiPCNA2_ARATH
AccessioniPrimary (citable) accession number: Q9ZW35
Secondary accession number(s): A0JQ96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: February 17, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.