Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cytosolic enolase 3

Gene

ENO3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase GAPC2, cytosolic (GAPC2), Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1)
  2. Phosphoglycerate kinase 3, cytosolic (PGK3)
  3. Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (At3g08590), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (PGM1)
  4. Enolase 1, chloroplastic (ENO1), Cytosolic enolase 3 (ENO3), Bifunctional enolase 2/transcriptional activator (ENO2)
  5. Pyruvate kinase (AXX17_At5g51800), Pyruvate kinase (AXX17_At3g24740), Pyruvate kinase (AXX17_At5g63250), Pyruvate kinase (AXX17_At3g50280), Pyruvate kinase (AXX17_ATUG04870), Pyruvate kinase (MAH20.13), Pyruvate kinase (MCD7.8), Pyruvate kinase (MBK5.16), Pyruvate kinase (F1I16_60), Pyruvate kinase (At3g25960), Pyruvate kinase (AXX17_At3g50440), Plastidial pyruvate kinase 3, chloroplastic (PKP3), Pyruvate kinase (At3g52990), Pyruvate kinase (AXX17_At5g08490), Pyruvate kinase (AXX17_At5g55530), Pyruvate kinase (AXX17_At3g28070), Pyruvate kinase (At2g36580), Pyruvate kinase (AXX17_At3g47400), Pyruvate kinase, Probable pyruvate kinase, cytosolic isozyme (At4g26390), Pyruvate kinase (F8J2_160), Pyruvate kinase (At3g52990), Pyruvate kinase (AXX17_At3g03460), Plastidial pyruvate kinase 2 (PKP2), Pyruvate kinase (AXX17_At2g33300), Pyruvate kinase (At3g04050), Pyruvate kinase (AXX17_At4g30430), Pyruvate kinase (F1I16_220), Plastidial pyruvate kinase 1, chloroplastic (PKP1), Pyruvate kinase (AXX17_At1g33230), Pyruvate kinase (At5g63680), Pyruvate kinase, Plastidial pyruvate kinase 4, chloroplastic (PKP4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei200SubstrateBy similarity1
Binding sitei209SubstrateBy similarity1
Active sitei252Proton donorBy similarity1
Metal bindingi287MagnesiumBy similarity1
Metal bindingi336MagnesiumBy similarity1
Binding sitei336SubstrateBy similarity1
Metal bindingi361MagnesiumBy similarity1
Binding sitei361SubstrateBy similarity1
Active sitei386Proton acceptorBy similarity1
Binding sitei437SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G29560-MONOMER
ReactomeiR-ATH-70171 Glycolysis
R-ATH-70263 Gluconeogenesis
UniPathwayiUPA00109; UER00187

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic enolase 3 (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase 3
2-phosphoglycerate dehydratase 3
Gene namesi
Name:ENO3
Synonyms:ENOC
Ordered Locus Names:At2g29560
ORF Names:F16P2.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G29560
TAIRilocus:2043067 AT2G29560

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00003995112 – 475Cytosolic enolase 3Add BLAST474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9ZW34
PRIDEiQ9ZW34

PTM databases

iPTMnetiQ9ZW34

Expressioni

Gene expression databases

ExpressionAtlasiQ9ZW34 baseline and differential
GenevisibleiQ9ZW34 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT2G29560.1

Structurei

3D structure databases

ProteinModelPortaliQ9ZW34
SMRiQ9ZW34
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni413 – 416Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

eggNOGiKOG2670 Eukaryota
COG0148 LUCA
HOGENOMiHOG000072174
InParanoidiQ9ZW34
KOiK01689
OMAiKEDWEHI
OrthoDBiEOG0936082Y
PhylomeDBiQ9ZW34

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ZW34-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVQEYLDKH MLSRKIEDAV NAAVRAKTSD PVLFIANHLK KAVSSVITKV
60 70 80 90 100
KARQILDSRG IPTVEVDLHT NKGVFRASVP SGDSSGTYEA IELRDGDKGM
110 120 130 140 150
YLGNSVAKAV KNINEKISEA LIGMDPKLQG QIDQAMIDLD KTEKKSELGA
160 170 180 190 200
NAILAVSIAA CKAGAAEKEV PLCKHLSDLS GRANMVLPVP AFTVLSGGKH
210 220 230 240 250
ASNTFAIQEI MILPIGASRF EEALQWGSET YHHLKAVISE KNGGLGCNVG
260 270 280 290 300
EDGGLAPDIS SLKEGLELVK EAINRTGYND KIKIAIDIAA TNFCLGTKYD
310 320 330 340 350
LDIKSPNKSG QNFKSAEDMI DMYKEICNDY PIVSIEDPFD KEDWEHTKYF
360 370 380 390 400
SSLGICQVVG DDLLMSNSKR VERAIQESSC NALLLKVNQI GTVTEAIEVV
410 420 430 440 450
KMARDAQWGV VTSHRCGETE DSFISDLSVG LATGVIKAGA PCRGERTMKY
460 470
NQLLRIEEEL GDQAVYAGED WKLSL
Length:475
Mass (Da):51,600
Last modified:May 1, 1999 - v1
Checksum:iDD09B5DC58A46B57
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004561 Genomic DNA Translation: AAC95183.1
CP002685 Genomic DNA Translation: AEC08273.1
AY035128 mRNA Translation: AAK59633.1
AY113918 mRNA Translation: AAM44966.1
PIRiG84697
RefSeqiNP_180516.1, NM_128509.4
UniGeneiAt.23904
At.64384

Genome annotation databases

EnsemblPlantsiAT2G29560.1; AT2G29560.1; AT2G29560
GeneIDi817505
GrameneiAT2G29560.1; AT2G29560.1; AT2G29560
KEGGiath:AT2G29560

Similar proteinsi

Entry informationi

Entry nameiENO3_ARATH
AccessioniPrimary (citable) accession number: Q9ZW34
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 1, 1999
Last modified: May 23, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health