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Protein

Cytosolic enolase 3

Gene

ENO3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase GAPC2, cytosolic (GAPC2), Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1)
  2. no protein annotated in this organism
  3. Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (At3g08590), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (PGM1)
  4. Enolase 1, chloroplastic (ENO1), Cytosolic enolase 3 (ENO3), Bifunctional enolase 2/transcriptional activator (ENO2)
  5. Pyruvate kinase (At5g08570), Pyruvate kinase (At5g56350), Pyruvate kinase (At3g25960), Pyruvate kinase (At5g63680), Pyruvate kinase (F1I16_60), Plastidial pyruvate kinase 3, chloroplastic (PKP3), Pyruvate kinase (At3g52990), Pyruvate kinase (At2g36580), Pyruvate kinase, Probable pyruvate kinase, cytosolic isozyme (At4g26390), Pyruvate kinase (F8J2_160), Pyruvate kinase (At3g52990), Plastidial pyruvate kinase 2 (PKP2), Pyruvate kinase (T11I18.16), Pyruvate kinase (F1I16_220), Plastidial pyruvate kinase 1, chloroplastic (PKP1), Pyruvate kinase (At5g63680), Pyruvate kinase, Plastidial pyruvate kinase 4, chloroplastic (PKP4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei200 – 2001SubstrateBy similarity
Binding sitei209 – 2091SubstrateBy similarity
Active sitei252 – 2521Proton donorBy similarity
Metal bindingi287 – 2871MagnesiumBy similarity
Metal bindingi336 – 3361MagnesiumBy similarity
Binding sitei336 – 3361SubstrateBy similarity
Metal bindingi361 – 3611MagnesiumBy similarity
Binding sitei361 – 3611SubstrateBy similarity
Active sitei386 – 3861Proton acceptorBy similarity
Binding sitei437 – 4371SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G29560-MONOMER.
UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic enolase 3 (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase 3
2-phosphoglycerate dehydratase 3
Gene namesi
Name:ENO3
Synonyms:ENOC
Ordered Locus Names:At2g29560
ORF Names:F16P2.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G29560.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • cytosol Source: TAIR
  • nucleus Source: TAIR
  • phosphopyruvate hydratase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 475474Cytosolic enolase 3PRO_0000399511Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9ZW34.
PRIDEiQ9ZW34.

PTM databases

iPTMnetiQ9ZW34.

Expressioni

Gene expression databases

GenevisibleiQ9ZW34. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT2G29560.1.

Structurei

3D structure databases

ProteinModelPortaliQ9ZW34.
SMRiQ9ZW34. Positions 47-472.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni413 – 4164Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

eggNOGiKOG2670. Eukaryota.
COG0148. LUCA.
HOGENOMiHOG000072174.
InParanoidiQ9ZW34.
KOiK01689.
OMAiVYAGEDW.
PhylomeDBiQ9ZW34.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SMARTiSM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiPS00164. ENOLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ZW34-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVQEYLDKH MLSRKIEDAV NAAVRAKTSD PVLFIANHLK KAVSSVITKV
60 70 80 90 100
KARQILDSRG IPTVEVDLHT NKGVFRASVP SGDSSGTYEA IELRDGDKGM
110 120 130 140 150
YLGNSVAKAV KNINEKISEA LIGMDPKLQG QIDQAMIDLD KTEKKSELGA
160 170 180 190 200
NAILAVSIAA CKAGAAEKEV PLCKHLSDLS GRANMVLPVP AFTVLSGGKH
210 220 230 240 250
ASNTFAIQEI MILPIGASRF EEALQWGSET YHHLKAVISE KNGGLGCNVG
260 270 280 290 300
EDGGLAPDIS SLKEGLELVK EAINRTGYND KIKIAIDIAA TNFCLGTKYD
310 320 330 340 350
LDIKSPNKSG QNFKSAEDMI DMYKEICNDY PIVSIEDPFD KEDWEHTKYF
360 370 380 390 400
SSLGICQVVG DDLLMSNSKR VERAIQESSC NALLLKVNQI GTVTEAIEVV
410 420 430 440 450
KMARDAQWGV VTSHRCGETE DSFISDLSVG LATGVIKAGA PCRGERTMKY
460 470
NQLLRIEEEL GDQAVYAGED WKLSL
Length:475
Mass (Da):51,600
Last modified:May 1, 1999 - v1
Checksum:iDD09B5DC58A46B57
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004561 Genomic DNA. Translation: AAC95183.1.
CP002685 Genomic DNA. Translation: AEC08273.1.
AY035128 mRNA. Translation: AAK59633.1.
AY113918 mRNA. Translation: AAM44966.1.
PIRiG84697.
RefSeqiNP_180516.1. NM_128509.3.
UniGeneiAt.23904.
At.64384.

Genome annotation databases

EnsemblPlantsiAT2G29560.1; AT2G29560.1; AT2G29560.
GeneIDi817505.
GrameneiAT2G29560.1; AT2G29560.1; AT2G29560.
KEGGiath:AT2G29560.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004561 Genomic DNA. Translation: AAC95183.1.
CP002685 Genomic DNA. Translation: AEC08273.1.
AY035128 mRNA. Translation: AAK59633.1.
AY113918 mRNA. Translation: AAM44966.1.
PIRiG84697.
RefSeqiNP_180516.1. NM_128509.3.
UniGeneiAt.23904.
At.64384.

3D structure databases

ProteinModelPortaliQ9ZW34.
SMRiQ9ZW34. Positions 47-472.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G29560.1.

PTM databases

iPTMnetiQ9ZW34.

Proteomic databases

PaxDbiQ9ZW34.
PRIDEiQ9ZW34.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G29560.1; AT2G29560.1; AT2G29560.
GeneIDi817505.
GrameneiAT2G29560.1; AT2G29560.1; AT2G29560.
KEGGiath:AT2G29560.

Organism-specific databases

TAIRiAT2G29560.

Phylogenomic databases

eggNOGiKOG2670. Eukaryota.
COG0148. LUCA.
HOGENOMiHOG000072174.
InParanoidiQ9ZW34.
KOiK01689.
OMAiVYAGEDW.
PhylomeDBiQ9ZW34.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.
BioCyciARA:AT2G29560-MONOMER.

Miscellaneous databases

PROiQ9ZW34.

Gene expression databases

GenevisibleiQ9ZW34. AT.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SMARTiSM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiPS00164. ENOLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Molecular and functional characterization of the plastid-localized Phosphoenolpyruvate enolase (ENO1) from Arabidopsis thaliana."
    Prabhakar V., Lottgert T., Gigolashvili T., Bell K., Flugge U.I., Hausler R.E.
    FEBS Lett. 583:983-991(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiENO3_ARATH
AccessioniPrimary (citable) accession number: Q9ZW34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 1, 1999
Last modified: May 11, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.