Q9ZW30 (GSTU1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase U1
Short name=AtGSTU1
EC=2.5.1.18

Alternative name(s):
GST class-tau member 1
Glutathione S-transferase 19

Gene names

Name:	GSTU1
Synonyms:	GST19
Ordered Locus Names:	At2g29490
ORF Names:	F16P2.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subcellular location	Cytoplasm › cytosol Probable.
Induction	By acetochlor, metolachlor and 2,4,6-trinitrotoluene (TNT). Ref.5
Sequence similarities	Belongs to the GST superfamily. Tau family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Biological process	Detoxification Stress response
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to stress Inferred from electronic annotation. Source: UniProtKB-KW response to toxin Inferred from electronic annotation. Source: UniProtKB-KW toxin catabolic process Traceable author statement Ref.1. Source: TAIR
Cellular_component	cytoplasm Non-traceable author statement Ref.1. Source: TAIR cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	Glutathione S-transferase U1		PRO_0000413548
Regions
	Domain	6 – 85	80	GST N-terminal
	Domain	90 – 217	128	GST C-terminal
	Region	16 – 17	2	Glutathione binding By similarity
	Region	42 – 43	2	Glutathione binding By similarity
	Region	56 – 57	2	Glutathione binding By similarity
	Region	69 – 70	2	Glutathione binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZW30 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 5BD722371683FED8	FASTA	224	25,914
	10 20 30 40 50 60 MAEKEESVKL LGFWASPFSR RVEMALKLKG VPYEYLEEDL PNKTPLLLEL NPLHKKVPVL 70 80 90 100 110 120 VHNDKILLES HLILEYIDQT WKNSPILPQD PYEKAMARFW AKFIDDQILT LGFRSLVKAE 130 140 150 160 170 180 KGREVAIEET RELLMFLEKE VTGKDFFGGK TIGFLDMIAG SMIPFCLARL WKGIGIDMIP 190 200 210 220 EEKFPELNRW IKNLEEVEAV RGCIPPREKQ IERMTKIAET IKSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Probing the diversity of the Arabidopsis glutathione S-transferase gene family." Wagner U., Edwards R., Dixon D.P., Mauch F. Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Gene expression and microscopic analysis of Arabidopsis exposed to chloroacetanilide herbicides and explosive compounds. A phytoremediation approach." Mezzari M.P., Walters K., Jelinkova M., Shih M.C., Just C.L., Schnoor J.L. Plant Physiol. 138:858-869(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF288183 mRNA. Translation: AAG30132.1. AC004561 Genomic DNA. Translation: AAC95189.1. CP002685 Genomic DNA. Translation: AEC08260.1. AF428387 mRNA. Translation: AAL16155.1. BT010162 mRNA. Translation: AAQ22631.1.
IPI	IPI00521899.
PIR	A84697.
RefSeq	NP_180510.1. NM_128503.2.
UniGene	At.20874.
3D structure databases
HSSP	HSSP built from PDB template 1OYJ based on UniProtKB O65032.
ProteinModelPortal	Q9ZW30.
SMR	Q9ZW30. Positions 4-209.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9ZW30. 6 interactions.
STRING	3702.AT2G29490.1-P.
Proteomic databases
PaxDb	Q9ZW30.
PRIDE	Q9ZW30.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G29490.1; AT2G29490.1; AT2G29490.
GeneID	817498.
KEGG	ath:AT2G29490.
Organism-specific databases
TAIR	At2g29490.
Phylogenomic databases
eggNOG	COG0625.
HOGENOM	HOG000125749.
InParanoid	Q9ZW30.
KO	K00799.
OMA	HLILEYI.
PhylomeDB	Q9ZW30.
ProtClustDB	CLSN2683712.
Gene expression databases
Genevestigator	Q9ZW30.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTU1_ARATH

Accession

Primary (citable) accession number: Q9ZW30

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2011
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program