Q9ZW29 (GSTU2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase U2
Short name=AtGSTU2
EC=2.5.1.18

Alternative name(s):
GST class-tau member 2
Glutathione S-transferase 20

Gene names

Name:	GSTU2
Synonyms:	GST20
Ordered Locus Names:	At2g29480
ORF Names:	F16P2.14

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subcellular location	Cytoplasm › cytosol Probable.
Sequence similarities	Belongs to the GST superfamily. Tau family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Biological process	Detoxification
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to toxin Inferred from electronic annotation. Source: UniProtKB-KW toxin catabolic process Traceable author statement Ref.1. Source: TAIR
Cellular_component	cytoplasm Non-traceable author statement Ref.1. Source: TAIR cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	Glutathione S-transferase U2		PRO_0000413549
Regions
	Domain	6 – 85	80	GST N-terminal
	Domain	90 – 217	128	GST C-terminal
	Region	16 – 17	2	Glutathione binding By similarity
	Region	42 – 43	2	Glutathione binding By similarity
	Region	56 – 57	2	Glutathione binding By similarity
	Region	69 – 70	2	Glutathione binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZW29 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: F7D2B7487AE877F4	FASTA	225	26,173
	10 20 30 40 50 60 MAKKEESVKL LGFWISPFSR RVEMALKLKG VPYEYLEEDL PKKSTLLLEL NPVHKKVPVL 70 80 90 100 110 120 VHNDKLLSES HVILEYIDQT WNNNPILPHD PYEKAMVRFW AKFVDEQILP VGFMPLVKAE 130 140 150 160 170 180 KGIDVAIEEI REMLMFLEKE VTGKDFFGGK TIGFLDMVAG SMIPFCLARA WECLGIDMTP 190 200 210 220 EDTFPELNRW IKNLNEVEIV RECIPPKEKH IERMKKIIER AKSTF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Probing the diversity of the Arabidopsis glutathione S-transferase gene family." Wagner U., Edwards R., Dixon D.P., Mauch F. Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF288184 mRNA. Translation: AAG30133.1. AC004561 Genomic DNA. Translation: AAC95190.1. CP002685 Genomic DNA. Translation: AEC08259.1. AY094455 mRNA. Translation: AAM19826.1. AY122905 mRNA. Translation: AAM67438.1.
IPI	IPI00529199.
PIR	H84696.
RefSeq	NP_180509.1. NM_128502.2.
UniGene	At.20874. At.66395.
3D structure databases
HSSP	HSSP built from PDB template 1OYJ based on UniProtKB O65032.
ProteinModelPortal	Q9ZW29.
SMR	Q9ZW29. Positions 4-209.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9ZW29. 4 interactions.
STRING	3702.AT2G29480.1-P.
Proteomic databases
PaxDb	Q9ZW29.
PRIDE	Q9ZW29.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G29480.1; AT2G29480.1; AT2G29480.
GeneID	817497.
KEGG	ath:AT2G29480.
Organism-specific databases
TAIR	At2g29480.
Phylogenomic databases
eggNOG	COG0625.
HOGENOM	HOG000125749.
InParanoid	Q9ZW29.
KO	K00799.
OMA	IREMLMF.
PhylomeDB	Q9ZW29.
ProtClustDB	CLSN2683712.
Gene expression databases
Genevestigator	Q9ZW29.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTU2_ARATH

Accession

Primary (citable) accession number: Q9ZW29

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2011
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program