Q9ZW28 (GSTU3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase U3
Short name=AtGSTU3
EC=2.5.1.18

Alternative name(s):
GST class-tau member 3
Glutathione S-transferase 21

Gene names

Name:	GSTU3
Synonyms:	GST21
Ordered Locus Names:	At2g29470
ORF Names:	F16P2.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subcellular location	Cytoplasm › cytosol Probable.
Sequence similarities	Belongs to the GST superfamily. Tau family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Biological process	Detoxification
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to toxin Inferred from electronic annotation. Source: UniProtKB-KW toxin catabolic process Traceable author statement Ref.1. Source: TAIR
Cellular_component	cytoplasm Non-traceable author statement Ref.1. Source: TAIR cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	Glutathione S-transferase U3		PRO_0000413550
Regions
	Domain	6 – 86	81	GST N-terminal
	Domain	91 – 218	128	GST C-terminal
	Region	16 – 17	2	Glutathione binding By similarity
	Region	43 – 44	2	Glutathione binding By similarity
	Region	57 – 58	2	Glutathione binding By similarity
	Region	70 – 71	2	Glutathione binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZW28 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 612AD3270285CD03	FASTA	225	25,941
	10 20 30 40 50 60 MAEKEEGVKL IGSWASPFSR RVEMALKLKG VPYDYLDEDY LVVKSPLLLQ LNPVYKKVPV 70 80 90 100 110 120 LVHNGKILPE SQLILEYIDQ TWTNNPILPQ SPYDKAMARF WAKFVDEQVT MIGLRSLVKS 130 140 150 160 170 180 EKRIDVAIEE VQELIMLLEN QITGKKLFGG ETIGFLDMVV GSMIPFCLAR AWEGMGIDMI 190 200 210 220 PEEKFPELNR WIKNLKEIEI VRECIPDREK HIEHMMKIVG RIKAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Probing the diversity of the Arabidopsis glutathione S-transferase gene family." Wagner U., Edwards R., Dixon D.P., Mauch F. Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF288185 mRNA. Translation: AAG30134.1. AC004561 Genomic DNA. Translation: AAC95191.1. CP002685 Genomic DNA. Translation: AEC08258.1. AK117612 mRNA. Translation: BAC42268.1.
IPI	IPI00547156.
PIR	G84696.
RefSeq	NP_180508.1. NM_128501.3.
UniGene	At.12689.
3D structure databases
HSSP	HSSP built from PDB template 1OYJ based on UniProtKB O65032.
ProteinModelPortal	Q9ZW28.
SMR	Q9ZW28. Positions 4-210.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G29470.1-P.
Proteomic databases
PaxDb	Q9ZW28.
PRIDE	Q9ZW28.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G29470.1; AT2G29470.1; AT2G29470.
GeneID	817496.
KEGG	ath:AT2G29470.
Organism-specific databases
TAIR	At2g29470.
Phylogenomic databases
eggNOG	NOG274516.
HOGENOM	HOG000125749.
InParanoid	Q9ZW28.
KO	K00799.
OMA	PDLCRWA.
PhylomeDB	Q9ZW28.
ProtClustDB	CLSN2683712.
Gene expression databases
Genevestigator	Q9ZW28.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTU3_ARATH

Accession

Primary (citable) accession number: Q9ZW28

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2011
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program