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Q9ZW27 (GSTU4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione S-transferase U4

Short name=AtGSTU4
EC=2.5.1.18
Alternative name(s):
GST class-tau member 4
Glutathione S-transferase 22
Gene names
Name:GSTU4
Synonyms:GST22
Ordered Locus Names:At2g29460
ORF Names:F16P2.16
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length224 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides By similarity.

Catalytic activity

RX + glutathione = HX + R-S-glutathione.

Subcellular location

Cytoplasmcytosol Probable.

Sequence similarities

Belongs to the GST superfamily. Tau family.

Contains 1 GST C-terminal domain.

Contains 1 GST N-terminal domain.

Ontologies

Keywords
   Biological processDetoxification
   Cellular componentCytoplasm
   Molecular functionTransferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processresponse to toxic substance

Inferred from electronic annotation. Source: UniProtKB-KW

toxin catabolic process

Traceable author statement Ref.1. Source: TAIR

   Cellular_componentcytoplasm

Non-traceable author statement Ref.1. Source: TAIR

cytosol

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionglutathione transferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 224224Glutathione S-transferase U4
PRO_0000413551

Regions

Domain6 – 8580GST N-terminal
Domain90 – 217128GST C-terminal
Region16 – 172Glutathione binding By similarity
Region42 – 432Glutathione binding By similarity
Region56 – 572Glutathione binding By similarity
Region69 – 702Glutathione binding By similarity

Amino acid modifications

Modified residue1511Phosphothreonine Ref.5

Experimental info

Sequence conflict1731G → D in AAK62449. Ref.4
Sequence conflict1731G → D in AAO30062. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9ZW27 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 3810B34656614BE0

FASTA22425,839
        10         20         30         40         50         60 
MAEKEEDVKL LGFWASPFTR RVEMAFKLKG VPYEYLEQDI VNKSPLLLQI NPVYKKVPVL 

        70         80         90        100        110        120 
VYKGKILSES HVILEYIDQI WKNNPILPQD PYEKAMALFW AKFVDEQVGP VAFMSVAKAE 

       130        140        150        160        170        180 
KGVEVAIKEA QELFMFLEKE VTGKDFFGGK TIGFLDLVAG SMIPFCLARG WEGMGIDMIP 

       190        200        210        220 
EEKFPELNRW IKNLKEIEIV RECIPPREEQ IEHMKKVVER IKSA 

« Hide

References

« Hide 'large scale' references
[1]"Probing the diversity of the Arabidopsis glutathione S-transferase gene family."
Wagner U., Edwards R., Dixon D.P., Mauch F.
Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-151, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF288186 mRNA. Translation: AAG30135.1.
AC004561 Genomic DNA. Translation: AAC95192.1.
CP002685 Genomic DNA. Translation: AEC08257.1.
AF387004 mRNA. Translation: AAK62449.1.
BT003399 mRNA. Translation: AAO30062.1.
PIRF84696.
RefSeqNP_180507.1. NM_128500.3.
UniGeneAt.12688.
At.67637.
At.68148.

3D structure databases

ProteinModelPortalQ9ZW27.
SMRQ9ZW27. Positions 6-221.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT2G29460.1-P.

Proteomic databases

PRIDEQ9ZW27.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G29460.1; AT2G29460.1; AT2G29460.
GeneID817495.
KEGGath:AT2G29460.

Organism-specific databases

TAIRAT2G29460.

Phylogenomic databases

eggNOGNOG288793.
HOGENOMHOG000125749.
InParanoidQ9ZW27.
KOK00799.
OMAEYLEQDI.
PhylomeDBQ9ZW27.

Enzyme and pathway databases

BioCycARA:AT2G29460-MONOMER.

Gene expression databases

GenevestigatorQ9ZW27.

Family and domain databases

Gene3D1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamPF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSTU4_ARATH
AccessionPrimary (citable) accession number: Q9ZW27
Secondary accession number(s): Q94EZ8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: May 1, 1999
Last modified: May 14, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names